Efficient and Reliable PCR Setup Using Eppendorf MasterMix
Christian Rohrer and Lars-Erik Peter
Christian Rohrer, Eppendorf AG, Germany
Lars-Erik Peters, Eppendorf 5Prime, USA
Introduction
The reliability of a PCR* experiment is dependent on its sensitivity,
...
Transfection Reagent Selector Kit Handbook
...
Label six 1.5-ml microfuge tubes AF for
mastermix preparation:
AC for DNA-Enhancer
mastermix preparation for Effectene Reagent
and DF for DNA
mastermix preparation for SuperFect Reagent.
...
TripleMaster PCR System
... Prepare two Mastermixes and keep them on ice.
mastermix 1 contains primers
and template.
mastermix 2 contains enzyme, dNTPs and buffer. Combine Maste...ed in the Appendix.
Set up
mastermix 1 in a sterile microcentrifuge tube, mix well, cen...
TripleMaster PCR System
... Prepare two Mastermixes and keep them on ice.
mastermix 1 contains primers
and template.
mastermix 2 contains enzyme, dNTPs and buffer. Combine Maste...ed in the Appendix.
Set up
mastermix 1 in a sterile microcentrifuge tube, mix well, cen...