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MacMelt in Biological Technology

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

...raphs for the 5' and 3' fragments generated after digestion of the apoB PCR product. 3 These graphs were produced using Bio-Rad macmelt software and were used to predict the percentage range of the denaturing gradient to be used in the gels. Multiple aliquots of t...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

...radients gels. The gel temperature conditions can be derived from the theoretical melt profile of a wild-type sequence using the macmelt or WinMelt software, therefore run conditions do not have to be determined empirically. Taken together, this makes TTGE a powerf...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

... an adaptation of Lermans MELT program created by Bio-Rad, called macmelt software. This study presents our efforts within a model system ...ns were calculated from the melting profile as determined by the macmelt software. The gradient gels were cast and samples were electrophoresed ...
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