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MMLV in Biological Technology

A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity

...Puro vector allow for transient or stable transfection of the shRNA as well as production of lentiviral particles. Unlike adenovirus or murine-based mmlv or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific shRNA construct into differentiate...

Recombinant HIV Reverse Transcriptase

... convenient 500 U size. A 10X Reaction Buffer is supplied with the enzyme. Figure 1. HIV RT Outperforms mmlv and AMV RT Enzymes in cDNA Synthesis at 50C. A synthetic RNA transcript for Xef-1 (1 g) was reverse transcribed with oligo(dT...

HIGH SENSITIVITY qRT-PCR

...e than SuperScript II and other RNase H mmlv reverse transcriptases Linear quanti... sensitivity using SuperScript II mmlv RNase H RT to amplify three... Ambion's MessageSensor RT Kit includes an mmlv RT that maintains full RNase H activity...

Optimized Dengue RT-PCR

... primer at 70C. Then 4 l of RT mix containing 2 l of 5X RT buffer, 0.5 l of 10mM dNTP, 1.0 l of sterile UHQ water and 100 U of mmlv reverse transcriptase (PROMEGA, USA) were added. The mixture was incubated at 37C for 1 hour for the RT reaction and then heated...

High-Titer Retroviral Vectors for Gene Delivery

...r the vector pLXSN, another commercially available mmlv vector. The vector pFB has been deleted for all ...e gag coding sequence relative to other mmlv vectors. 5 This extended packaging sig...into the pFB and pFB-Neo vectors. In addition, the mmlv splice acceptor (SA) sequence in pMFG h...

Generate High-Quality, Directional Plasmid cDNA Libraries

... system, directional cDNA synthesis 1 is optimized using cloned mmlv reverse transcriptase to synthesize a greater yield of high-mole...n the proper orientation for protein expression. In this method, mmlv reverse transcriptase is used to synthesize first-strand cDNA fr...

pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

...roviral 5 promoter is then inactive in infected cells. The rationale for this construction is the following: The CMV promoter is stronger than the mmlv LTR and is persistently active in a wider range of cell types, and inactivation of the 5 LTR obviates potential interference with the CMV promoter...

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

...r not only with respect to the RT and PCR enzymes employed, but also with the procedures whereby cDNA synthesis and PCR amplification are coupled. mmlv reverse transcriptase (MMLV-RT), AMV RT, and Tth DNA polymerase are most commonly used to perform cDNA synthesis. 1,2,3,4 Second-strand synthesis...

Safe Production of High-Titer Retrovirus

...gned with four aims in mind: High Titer: mmlv gag-pol fusion protein and each of the envelope ...erlap between the packaging vectors and most mmlv retroviral vectors, the probability of generating ... were constructed by preserving all or most of the mmlv genomic DNA and deleting the packaging site so t...
MMLV in Biological Products

GeneChoice RNA Safe Recombinant Ribonuclease Inhibitor from GeneChoice, Inc.

Description:... interfere with the performance of other enzymes in the reaction. • RNA Safe is most effective between pH 5.5 and 8.0. It does not inhibit AMV or mmlv reverse transcriptases; SP6, T3, and T7 RNA polymerases; Taq DNA polymerase; RNase H; RNase T1; or S1 Nuclease. • Efficient inhibition of ribonu...
Company:GeneChoice, Inc.
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(Date:4/23/2014)... Effects of Midlife Occupational and Leisure Time Physical Activity ... physical activity in midlife increases the risk of mobility ... the risk. This is found in a study which ... The study was conducted at the Gerontology Research Center ... , Heavy physical labor is often repetitive, wears the ...
(Date:4/23/2014)... disease has been a rapidly evolving pursuit with ... crook in the research road, scientists have found ... associated with the disease. The report, which appears ... could have important implications for developing novel treatments. ... explain that for years, research has suggested a ...
(Date:4/22/2014)... decades researchers have characterized a set of clock genes ... all types of species, from flies to humans. Over ... surmise there are more. A team from the Perelman ... if big-data approaches could find them. , To ... PhD, professor of Pharmacology and first author Ron Anafi, ...
(Date:4/22/2014)... The risk of pregnancy among women using a ... more than 10 times greater over a 10-year period ... study by researchers at Yale University and UC Davis ... medical journal Contraception , the study found the ... marketed under the brand name Essure. , "This ...
(Date:4/22/2014)... NY, April 22, 2014Hemoglobin A1c is the standard measurement ... diabetes. Blood levels of A1c are typically measured every ... developed a data-based model that accurately estimates A1c using ... Diabetes Technology & Therapeutics ( DTT ), a ... article is available free on the DTT ...
Breaking Medicine News(10 mins):Health News:Midlife occupational and leisure-time physical activity limits mobility in old age 2Health News:Bioinformatics profiling identifies a new mammalian clock gene 2Health News:Risk of pregnancy greater with newer method of female sterilization 2Health News:Risk of pregnancy greater with newer method of female sterilization 3
(Date:4/23/2014)... 23, 2014 A biomedical engineer at the University ... technology to make blood transfusions safer. His work is ... Institutes of Health (NIH). , Blood transfusions save ... modern medicine,s absolute necessities. Without them, for instance, routine ... are perfect, however. There,s strong evidence that transfusions of ...
(Date:4/23/2014)... is leading a 2 million Food Standards Agency (FSA) ... premises and industry workers. , Norovirus outbreaks can ... a batch of frozen strawberries infected 11,000 people in ... understanding of which strains cause infection and which foods ... , Researchers will produce data that will help ...
(Date:4/22/2014)... Fla. (April 21, 2014) -- There are all sorts ... feathers and spread their colorful tails; satin bowbirds build ... blue and shiny objects; and European bitterling males show ... has evolved a unique method to communicate with others. ... "Signaling can have profound fitness implications for ...
Breaking Biology News(10 mins):UH biomedical engineer works to make blood transfusions safer 2UH biomedical engineer works to make blood transfusions safer 3Best practices in communication for the animal world 2Best practices in communication for the animal world 3
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