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MMLV in Biological Technology

A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity

...Puro vector allow for transient or stable transfection of the shRNA as well as production of lentiviral particles. Unlike adenovirus or murine-based mmlv or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific shRNA construct into differentiate...

Recombinant HIV Reverse Transcriptase

... convenient 500 U size. A 10X Reaction Buffer is supplied with the enzyme. Figure 1. HIV RT Outperforms mmlv and AMV RT Enzymes in cDNA Synthesis at 50C. A synthetic RNA transcript for Xef-1 (1 g) was reverse transcribed with oligo(dT...

HIGH SENSITIVITY qRT-PCR

...e than SuperScript II and other RNase H mmlv reverse transcriptases Linear quanti... sensitivity using SuperScript II mmlv RNase H RT to amplify three... Ambion's MessageSensor RT Kit includes an mmlv RT that maintains full RNase H activity...

Optimized Dengue RT-PCR

... primer at 70C. Then 4 l of RT mix containing 2 l of 5X RT buffer, 0.5 l of 10mM dNTP, 1.0 l of sterile UHQ water and 100 U of mmlv reverse transcriptase (PROMEGA, USA) were added. The mixture was incubated at 37C for 1 hour for the RT reaction and then heated...

High-Titer Retroviral Vectors for Gene Delivery

...r the vector pLXSN, another commercially available mmlv vector. The vector pFB has been deleted for all ...e gag coding sequence relative to other mmlv vectors. 5 This extended packaging sig...into the pFB and pFB-Neo vectors. In addition, the mmlv splice acceptor (SA) sequence in pMFG h...

Generate High-Quality, Directional Plasmid cDNA Libraries

... system, directional cDNA synthesis 1 is optimized using cloned mmlv reverse transcriptase to synthesize a greater yield of high-mole...n the proper orientation for protein expression. In this method, mmlv reverse transcriptase is used to synthesize first-strand cDNA fr...

pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

...roviral 5 promoter is then inactive in infected cells. The rationale for this construction is the following: The CMV promoter is stronger than the mmlv LTR and is persistently active in a wider range of cell types, and inactivation of the 5 LTR obviates potential interference with the CMV promoter...

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

...r not only with respect to the RT and PCR enzymes employed, but also with the procedures whereby cDNA synthesis and PCR amplification are coupled. mmlv reverse transcriptase (MMLV-RT), AMV RT, and Tth DNA polymerase are most commonly used to perform cDNA synthesis. 1,2,3,4 Second-strand synthesis...

Safe Production of High-Titer Retrovirus

...gned with four aims in mind: High Titer: mmlv gag-pol fusion protein and each of the envelope ...erlap between the packaging vectors and most mmlv retroviral vectors, the probability of generating ... were constructed by preserving all or most of the mmlv genomic DNA and deleting the packaging site so t...
MMLV in Biological Products

GeneChoice RNA Safe Recombinant Ribonuclease Inhibitor from GeneChoice, Inc.

Description:... interfere with the performance of other enzymes in the reaction. • RNA Safe is most effective between pH 5.5 and 8.0. It does not inhibit AMV or mmlv reverse transcriptases; SP6, T3, and T7 RNA polymerases; Taq DNA polymerase; RNase H; RNase T1; or S1 Nuclease. • Efficient inhibition of ribonu...
Company:GeneChoice, Inc.
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