A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity
...Puro vector allow for transient or stable transfection of the shRNA as well as production of lentiviral particles. Unlike adenovirus or murine-based mmlv
or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific shRNA construct into differentiate...
Recombinant HIV Reverse Transcriptase
... convenient 500 U size. A 10X Reaction Buffer is supplied
with the enzyme.
Figure 1. HIV
RT Outperforms mmlv
and AMV RT Enzymes in cDNA Synthesis at 50C.
A synthetic RNA transcript for Xef-1
(1 g) was reverse transcribed with oligo(dT...
HIGH SENSITIVITY qRT-PCR
...e than SuperScript
II and other RNase H mmlv
Linear quanti... sensitivity using SuperScript II mmlv
RNase H RT
to amplify three...
Ambion's MessageSensor RT Kit includes an mmlv
that maintains full RNase H activity...
Optimized Dengue RT-PCR
... primer at 70C. Then 4 l of RT mix containing 2 l of
5X RT buffer, 0.5 l of 10mM dNTP, 1.0 l of sterile UHQ water
and 100 U of mmlv
reverse transcriptase (PROMEGA, USA) were added. The
mixture was incubated at 37C for 1 hour for the RT reaction and then
Retroviral Vectors for Gene Delivery
...r the vector pLXSN, another commercially available mmlv
vector. The vector pFB
has been deleted for all ...e gag
coding sequence relative to other mmlv
vectors. 5 This extended
packaging sig...into the pFB and pFB-Neo vectors. In addition, the mmlv
(SA) sequence in pMFG h...
Generate High-Quality, Directional Plasmid cDNA Libraries
... system, directional cDNA synthesis 1 is optimized
using cloned mmlv
reverse transcriptase to synthesize a greater yield
of high-mole...n the proper orientation for protein expression. In this
reverse transcriptase is used to synthesize first-strand
pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
promoter is then inactive in infected cells. The rationale for this construction
is the following: The CMV promoter is stronger than the mmlv
LTR and is
persistently active in a wider range of cell types, and inactivation of the 5
LTR obviates potential interference with the CMV promoter...
prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
...r not only with respect to the RT and PCR enzymes employed,
but also with the procedures whereby cDNA synthesis and PCR amplification are
reverse transcriptase (MMLV-RT), AMV RT, and Tth DNA
polymerase are most commonly used to perform cDNA synthesis. 1,2,3,4
Safe Production of High-Titer Retrovirus
...gned with four aims in mind:
High Titer: mmlv
gag-pol fusion protein and each of the envelope
...erlap between the packaging
vectors and most mmlv
retroviral vectors, the probability of generating
... were constructed by preserving all or most of the mmlv
and deleting the packaging site so t...