Production of Full-Length, Biologically Active MEK1WT and MEK1CA
Useful tools for signal transduction research and drug screening Quinn Lu Chao-Feng Zheng Stratagene Cloning Systems, Inc. Stratagene has successfully expressed and purified recombinant, wild-type human MEK1 WT and a constitutively active mutant (MEK1 CA ) using the E...MEK1 and MEK2 regulate distinct functions by sorting ERK2 to different ...
a–c) Control and 5 min of EGF-stimulated cells expressing MEK1wt. ... In control cells transfected with MEK1wt, ERK2wt cytoplasmic staining was found ...FASEB J. -- Skarpen et al. 22 (2): 466 Figure 5
... with ERK2-NES-GFP or ERK2-AAA-GFP combined with MEK1wt together with Racwt. ... either MEK1wt or MEK2wt with and without EGF with an antibody recognizing T-E ...Dual Control of Muscle Cell Survival by Distinct Growth Factor ...
... encoding either a constitutively active Mek1 (Mek1*) or wild-type enzyme (Mek1WT) ... transfected with recombinant expression plasmids for either Mek1* or Mek1WT. ...Novel MEK1 Mutation Identified by Mutational Analysis of Epidermal ...
MEK1WT and MEK1K57N cDNAs were cloned into pLP-CMVneo plasmid using the Creator ... Ba/F3 cells carrying either MEK1WT or MEK1K57N, respectively, were plated in ...Constitutive activation of MEK1 in chondrocytes causes Stat1 ...
Journal List > Genes Dev > v.18(3); Feb 1, 2004. Abstract. Full ... Production of full-length, biologically active MEK1WT and MEK1CA. Strategies 10: 46-47. ...Dynamics of the Ras/ERK MAPK Cascade as Monitored by Fluorescent Probes ...
Submit a Letter to Editor. Alert me when this article is cited. Alert me ... pCAGGS-FLAG-ERK2-m1YFP, pCAGGS-FLAG-MEK1WT-mRFP-pm