Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit

... the fluorescent label, Cy3, to long dsRNA prepared with the megascript RNAi Kit and then compared labeled dsRNA activity to that of... href="http://www.ambion.com/catalog/CatNum.php?1626">1626 megascript RNAi Kit 20 rxns

TURBO DNase: Hyperactive, Salt Tolerant DNase

...ion or Promega (0.05 U), or Ambion's TURBO DNase (0.05 U) were added to a 20 l reaction containing 1 g of lamda DNA and Ambion's megascript T7 In Vitro Transcription Buffer at a 1X concentration. The reaction was incubated for 13 min at 25C, quenched with EDTA, and im...

Synthesize High Yields of Biotinylated aRNA

... Size 1334 megascript T7 Kit 40 rxns 1751 ...

RNAi: Size Does Matter

... (Figure 1A), were prepared using the megascript RNAi Kit and tested on Drosophil... yields of dsRNA synthesized and purified with the megascript RNAi Kit are reproducibly higher for sh... Yields of dsRNA synthesized and purified with the megascript RNAi kit using 0.4 g of PCR DNA templat...

RNAi: A How To for New Users

... target transcript is used to induce RNAi. The dsRNA can be readily generated by in vitro transcription. The megascript RNAi Kit was developed specifically for this purpose. dsRNA design for inducing RNAi in many non-mammalian s...

RNAi in Non-Mammalian Cultured Cells

...an now be easily prepared using the megascript RNAi Kit. The kit is based on Amb... of RNAi experiments in these organisms. The megascript RNAi Kit, based on Ambion's patented megascript large scale transcription technolog...

RNAi for High Throughput Analysis

...rge number of samples. Although the megascript RNAi Kit includes reagents for both dsRNA producti...ns, we recommend combining Ambion's megascript High Yield Transcription Kit with M...RNAs prepared and purified with the megascript RNAi Kit was observed. ...

RNA Amplification Just Got Better: MessageAmp II

...mproved Amplification Kit The RT and second-strand cDNA synthesis improvements, combined with the use of megascript technology in the in vitro transcription reaction (IVT) result in the ability to use shortened IVT incubatio...

High Throughput aRNA Amplification

...cript, an engineered M-MLV RT, to maximize yields of full-length cDNA aRNA synthesis powered by megascript High Yield Transcription Kit, the best technology for in vitro transcription High labeling efficiency ...

High Yield Transcription for Every Application

...gh Yield Transcription The megascript Kit is the most frequently ci... using 1, 4, or 9 kb templates with Ambion's megascript Kit according to protocol. 20 l reactio...yield. Figure 2. megascript vs. Conventional In Vitro Transcription...

MEGAscript in Biological Products

MEGAscript RNAi Kit from Ambion

Description:... The megascript RNAi Kit is optimized for the synthesis of large mass amounts of ready-to-use dsRNA for RNAi experiments in non-mammalian systems. The megascript RNAi Kit is based on Ambions patented high-yield transcription technol-ogy ...
Company:Ambion