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MEGAscript in Biological Technology

Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit

... the fluorescent label, Cy3, to long dsRNA prepared with the megascript RNAi Kit and then compared labeled dsRNA activity to that of... href="http://www.ambion.com/catalog/CatNum.php?1626">1626 megascript RNAi Kit 20 rxns

TURBO DNase: Hyperactive, Salt Tolerant DNase

...ion or Promega (0.05 U), or Ambion's TURBO DNase (0.05 U) were added to a 20 l reaction containing 1 g of lamda DNA and Ambion's megascript T7 In Vitro Transcription Buffer at a 1X concentration. The reaction was incubated for 13 min at 25C, quenched with EDTA, and im...

Synthesize High Yields of Biotinylated aRNA

... Size 1334 megascript T7 Kit 40 rxns 1751 ...

RNAi: Size Does Matter

... (Figure 1A), were prepared using the megascript RNAi Kit and tested on Drosophil... yields of dsRNA synthesized and purified with the megascript RNAi Kit are reproducibly higher for sh... Yields of dsRNA synthesized and purified with the megascript RNAi kit using 0.4 g of PCR DNA templat...

RNAi: A How To for New Users

... target transcript is used to induce RNAi. The dsRNA can be readily generated by in vitro transcription. The megascript RNAi Kit was developed specifically for this purpose. dsRNA design for inducing RNAi in many non-mammalian s...

RNAi in Non-Mammalian Cultured Cells

...an now be easily prepared using the megascript RNAi Kit. The kit is based on Amb... of RNAi experiments in these organisms. The megascript RNAi Kit, based on Ambion's patented megascript large scale transcription technolog...

RNAi for High Throughput Analysis

...rge number of samples. Although the megascript RNAi Kit includes reagents for both dsRNA producti...ns, we recommend combining Ambion's megascript High Yield Transcription Kit with M...RNAs prepared and purified with the megascript RNAi Kit was observed. ...

RNA Amplification Just Got Better: MessageAmp II

...mproved Amplification Kit The RT and second-strand cDNA synthesis improvements, combined with the use of megascript technology in the in vitro transcription reaction (IVT) result in the ability to use shortened IVT incubatio...

High Throughput aRNA Amplification

...cript, an engineered M-MLV RT, to maximize yields of full-length cDNA aRNA synthesis powered by megascript High Yield Transcription Kit, the best technology for in vitro transcription High labeling efficiency ...

High Yield Transcription for Every Application

...gh Yield Transcription The megascript Kit is the most frequently ci... using 1, 4, or 9 kb templates with Ambion's megascript Kit according to protocol. 20 l reactio...yield. Figure 2. megascript vs. Conventional In Vitro Transcription...
MEGAscript in Biological Products

MEGAscript RNAi Kit from Ambion

Description:... The megascript RNAi Kit is optimized for the synthesis of large mass amounts of ready-to-use dsRNA for RNAi experiments in non-mammalian systems. The megascript RNAi Kit is based on Ambions patented high-yield transcription technol-ogy ...
Company:Ambion
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(Date:4/18/2014)... of your worst memories? How did it make you ... a negative personal experience, such as how sad you ... emotional distress, especially when you can,t stop thinking about ... thinking about the context of the memories, rather than ... way to alleviate the negative effects of these memories, ...
(Date:4/18/2014)... available in French . ... practice and regenerative medicine. A team led by Ludwik ... Paris Tech) and Didier Letourneur from the Laboratoire Recherche ... just demonstrated that the principle of adhesion by aqueous ... repair soft-tissue organs and tissues. This easy-to-use gluing method ...
(Date:4/17/2014)... first genetic variant specifically associated with the risk ... 10-15 per cent of all breast cancer cases. ... cancer sub-type, called invasive lobular carcinoma, gives researchers ... particular kind of breast cancer, which can be ... (Thursday) in the journal PloS Genetics , ...
(Date:4/17/2014)... the April issue of Experimental Biology and ... Drs. Rex Gaskins and Paul Kenis in the ... of the University of Illinois Urbana-Champaign describe their ... reactions underlie a variety of cell functions including ... these crucial roles in regulating normal cellular behavior, ...
(Date:4/17/2014)... Children,s Hospital Pediatrician-in-Chief Jon McCullers, MD, was recently ... issue of Nature Reviews Microbiology , one ... McCullers, a world-renowned infectious disease specialist, and chair ... of Tennessee Health Science Center, analyzed the epidemiology ... and 1968 pandemics, as well as more recent ...
Breaking Medicine News(10 mins):Health News:New study suggests a better way to deal with bad memories 2Health News:New study suggests a better way to deal with bad memories 3Health News:Innovative strategy to facilitate organ repair 2Health News:Innovative strategy to facilitate organ repair 3Health News:First genetic link discovered to difficult-to-diagnose breast cancer sub-type 2Health News:First genetic link discovered to difficult-to-diagnose breast cancer sub-type 3Health News:Live cell imaging reveals distinct alterations of subcellular glutathione potentials 2
(Date:4/17/2014)... painted a stark warning on the possible effects of ... effect 32 times that of carbon dioxide. Now a ... act as fully regenerable electron acceptors which helps explain ... instead of being released to the atmosphere. However, there ... may enter into a vicious cycle to release large ...
(Date:4/17/2014)... full of wild animals and plant life, but there,s ... churning away in the soil, decomposing organic matter and ... role these fungi play in ecological systems, their identities ... scientists has generated a genetic map of more than ... was published this week in the Proceedings of ...
(Date:4/16/2014)... legal, artisanal green sea turtle fishery has uncovered a ... in what may have become an unsustainable take, according ... University of Florida. , During the research period, conservation ... killed between 1991 and 2011, with catch rates peaking ...
Breaking Biology News(10 mins):Methane climate change risk suggested by proof of redox cycling of humic substances 2Stanford biologists help solve fungal mysteries 2Stanford biologists help solve fungal mysteries 3Declining catch rates in Caribbean green turtle fishery may be result of overfishing 2Declining catch rates in Caribbean green turtle fishery may be result of overfishing 3
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