Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
J. Samskog, H. Wadensten, and J. Flensburg GE Healthcare, Uppsala, Sweden A 2D–LC-MS method was developed to analyse phosphopeptides in mouse brain tissue. The trypsin-digested tissue was separated by strong cation exchange chromatography (SCX),...2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
Key words: phosphopeptide • two-dimensional liquid chromatography (2D-LC) • Ettan MDLC • LC-MS/MS • reversed-phase chromatography (RPC) • strong cation exchange (SCX) chromatography • linear ion trap mass spectrometer A 2D...MIDAS TRAQ System with 4000 Q TRAP LC/MS/MS System from Applied Biosystems
Description:...RAQ systems integrate the unique quantitative and qualitative capabilities of the 4000 Q TRAP and 3200 Q TRAP LC/MS/MS Systems with the new Tempo nano mdlc System plus automation software, to provide a rapid solution for high sensitivity validation of protein biomarkers. Eliminate extensive sample pr...