Rapid PCR* Using Active Tube Temperature Control On Thermo's PCR Machines

...e PCR programme was successfully run on Thermo's mbs and Px2 thermal cyclers. Yield of PCR product wa...y caused an increase in the run time on both the mbs and the Px2 (table 1). In these equivalent proto...a comprise on yield quality for both the Px2 and mbs instruments. Conclu...

High-Titer Retroviral Vectors for Gene Delivery

...as used for virus production. Producer cells were transiently transfected with both of the GFP constructs using the Transfection mbs mammalian transfection kit (Stratagene) with some modifications. 4 Two days following transfection, viral supernatants were collected, and...

hNT BlueNeurons Express b-Galactosidase at High Levels

... 3 NT2 cells were transfected with the pSNRSV expression vector as prescribed by the manual for Stratagene's Transfection mbs Mammalian Transfection Kit. Forty-eight hours posttransfection, cells were split 1:40 and after an additional 24 hours, G418 was...

Versatile Reporter Vectors for Monitoring Viral Transduction

...nd VSV-G env expression vectors pVPack-GP and pVPack-VSV-G. 3 (Figure 1B) Transfections were carried out using the Transfection mbs mammalian transfection kit (Stratagene), modified according to Pear et. al. 4 Figure 2 shows that HeLa cells transduced with the...

Safe Production of High-Titer Retrovirus

...ves work especially well when used with Stratagenes Transfection mbs transfection kit, modified according to Pear and colleagues 6 (...k-VSV-G, pVPack-Ampho, pVPack-Eco or pVPack-10A1), using the Transfection mbs transfection protocol (Stratagene) modified according to Pear et al. 6 F...

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

...ant adenoviral plasmid clones were identified, they were digested with Pac I and transfected into HEK293 cells using Stratagenes mbs Mammalian Transfection Kit, with modifications described by Pear and colleagues. 4 HEK293 cells are human embryonic kidney cell...

Human Primary Preadipocytes and Differentiated Adipocytes

...ove the transfectability of Human Differentiated Adipocytes or Human Predifferentiated Adipocytes using Stratagenes Transfection mbs Mammalian Transfection Kit or LipoTAXI Transfection Reagent, respectively, we showed that including the Primary Transfection E...