Rapid PCR* Using Active Tube Temperature Control On Thermo's PCR Machines
...e PCR programme
was successfully run on Thermo's mbs
Px2 thermal cyclers. Yield of PCR product wa...y caused an increase in the run
time on both the mbs
and the Px2 (table 1).
In these equivalent proto...a comprise on
yield quality for both the Px2 and mbs
Retroviral Vectors for Gene Delivery
...as used for virus production. Producer cells
were transiently transfected with both of the GFP constructs using the
mammalian transfection kit (Stratagene) with some modifications. 4
Two days following transfection, viral supernatants were collected, and...
hNT BlueNeurons Express b-Galactosidase at High
NT2 cells were transfected with the pSNRSV expression vector as prescribed
by the manual for Stratagene's Transfection mbs
Kit. Forty-eight hours posttransfection, cells were split 1:40 and after
an additional 24 hours, G418 was...
Versatile Reporter Vectors for Monitoring Viral Transduction
...nd VSV-G env expression vectors
pVPack-GP and pVPack-VSV-G. 3 (Figure
1B) Transfections were carried out using the Transfection mbs
transfection kit (Stratagene), modified according to Pear et. al. 4
Figure 2 shows that HeLa cells transduced with the...
Safe Production of High-Titer Retrovirus
especially well when used with Stratagenes Transfection mbs
kit, modified according to Pear and colleagues 6 (...k-VSV-G, pVPack-Ampho, pVPack-Eco or pVPack-10A1), using the
transfection protocol (Stratagene) modified according to Pear
et al. 6 F...
Generate Adenovirus Vectors in E. coli by Homologous Recombination
with the AdEasy Adenoviral Vector System
...ant adenoviral plasmid clones were identified, they were
digested with Pac I and transfected into HEK293 cells using Stratagenes
Mammalian Transfection Kit, with modifications described by Pear and
colleagues. 4 HEK293 cells are human embryonic kidney
Human Primary Preadipocytes and Differentiated Adipocytes
...ove the transfectability
of Human Differentiated Adipocytes or Human Predifferentiated Adipocytes
using Stratagenes Transfection mbs
Mammalian Transfection Kit or
LipoTAXI Transfection Reagent, respectively, we showed that
including the Primary Transfection E...