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Lysis in Medical News

New Treatment For Patients With Paralysis

Investigators designed a combination therapy and tested it on rats with spinal cord contusions -- the most common cause of paralysis in humans.// The combination therapy consisted of transplanted Schwann cells, which is a type of cell that insulates nerve cells, a cell messenger molecule called cyc...

Journal of Clinical Oncology Publishes TREANDA Study Demonstrating Significant Improvement in Overall Response and Progression-Free Survival in Chronic Lymphocytic Leukemia

...erious adverse reactions have been associated with TREANDA in clinical trials: myelosuppression, infections, infusion reactions and anaphylaxis, tumor lysis syndrome, skin reactions, and other malignancies. TREANDA has a unique chemical structure that is synthesized to combine an alkylating group an...

MO BIO Laboratories, Inc. Launches PowerFood(TM) Microbial DNA Isolation Kit

...athogens. Further, it is the only complete kit on the market, eliminating the need to purchase additional reagents for post-purification clean up and lysis enzymes. The PowerFood Microbial DNA Isolation Kit incorporates patented Inhibitor Removal Technology(R) (IRT) to remove PCR-inhibiting compounds ass...

Pharmacomechanical Thrombectomy Procedure Adoption to Accelerate Due to More Aggressive Treatment of Deep Vein Thrombosis, According to Millennium Research Group

...which is slated to begin recruitment in July 2009. "Firms are hoping that results of this trial will clarify the association between rapid clot lysis and the prevention of post-thrombotic syndrome in patients with proximal DVT, as well as demonstrate how PMT may have lower bleeding risks compared wi...

NeuroTherm Completes Acquisition of the Micron Epidural Catheter

...g for us to provide an epidural catheter as part of our product mix," continues Davis. The Micron Catheter is used in a procedure called Epidural lysis of Adhesions; a common interventional pain procedure used to remove scar tissue. The scar tissue which typically result from back surgery, inflame su...

Early treatment of systemic onset JIA with anakinra restores the IL-18 response

...During the six month study, numbers of NK cells slightly increased initially but remained stable throughout treatment with anakinra, although specific lysis (the death of a cell by breaking of the cellular membrane) was restored to normal within just three days. Interestingly, the NK cell response after IL...

JCI online early table of contents: April 6, 2009

... melanoma cells and suggest that NK cells could be harnessed to prevent melanoma metastasis. TITLE: NCRs and DNAM-1 mediate NK cell recognition and lysis of human and mouse melanoma cell lines in vitro and in vivo AUTHOR CONTACT: Francesco Colucci The Babraham Institute, Cambridge, United Kingdom. ...

New NCCN Guidelines for Primary Cutaneous B-Cell Lymphoma and Updates to NCCN Guidelines for Non-Hodgkin's Lymphomas

... on supportive care for patients with CLL. Lastly, Dr. Zelenetz touched upon a new section detailing hallmarks, symptoms, and treatment for Tumor lysis Syndrome -- a group of metabolic complications that can occur after the treatment of lymphoma. About the National Comprehensive Cancer Network ...

Data on VELCADE(R) (Bortezomib) for Injection Based Therapies to be Presented at the International Myeloma Workshop

...diac and pulmonary disorders, reversible posterior leukoencephalopathy syndrome, gastrointestinal adverse events, thrombocytopenia, neutropenia, tumor lysis syndrome and hepatic events. Women of childbearing potential should avoid becoming pregnant while being treated with VELCADE. Nursing mothers are advi...

GlaxoSmithKline and Genmab Submit Arzerra(TM) (Ofatumumab) Application to FDA for the Treatment of Advanced Stage Blood Cancer

...ase of progressive multifocal leukoencephalopathy (PML), a rare brain infection resulting in death or causing severe disability, and one case of tumor lysis syndrome were reported.(5,6) These data were presented at the 50th Annual Meeting of the American Society of Hematology (ASH) in December 2008. ...

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Lysis in Medical Technology

Phase III Trial Results Show Elitek(R) (rasburicase) Significantly Reduced Plasma Uric Acid Levels versus Allopurinol in Adults with Hematologic Cancers at Risk for Tumor Lysis Syndrome

- Results Presented at ASH Annual Meeting- SAN FRANCISCO, Dec. 6 /PRNewswire-FirstCall/ -- Sanofi-aventis today announced results of a randomized phase III study presented at the 50th Annual Meeting of the American Society of Hematology. The study in adult patients with hematological mali...

Micromet Presents Preclinical Data Showing Activity of Erbitux and Vectibix Based BiTE Antibodies against KRAS- and BRAF-mutated Human Colorectal Cancer Cells

...antibody for the treatment of patients with KRAS- and BRAF-mutated colorectal cancer is now possible." (1)Lutterbuese, R. et al. Highly efficient lysis of KRAS- and BRAF-mutated human colon cancer cells by T cell-engaging BiTE antibodies derived from anti-EGFR antibodies cetuximab and panitumumab AAC...

Jennerex Treats First Patients in Phase 2 JX-594 Trial for Liver Cancer

... high levels in cancer cells. To enhance product efficacy, JX-594 is also engineered to express the GM-CSF protein. GM-CSF complements the cancer cell lysis work of the product candidate, leading to a cascade of events resulting in tumor necrosis, tumor vasculature shutdown and an anti-tumoral immune attac...

Continued Overall Survival Advantage Reported From Pivotal Trial of VELCADE(R) (Bortezomib) for Injection Based Therapy in Patients With Previously Untreated Multiple Myeloma

...diac and pulmonary disorders, reversible posterior leukoencephalopathy syndrome, gastrointestinal adverse events, thrombocytopenia, neutropenia, tumor lysis syndrome and hepatic events. Women of childbearing potential should avoid becoming pregnant while being treated with VELCADE. Nursing mothers are advi...

New Study Shows PCR and FCR Have Significant Activity in B-cell Chronic Lymphocytic Leukemia

...wn: Superiority of Rasuburicase vs. Allopurinol on serum uric acid control in adult patients with hematologic malignancies at risk of developing Tumor lysis Syndrome Multiple Myeloma Jeffrey Matous, M.D., Rocky Mountain Cancer Centers-Midtown: Phase II study of...

Prolonged Progression-Free Survival Demonstrated by VELCADE(R) (Bortezomib) for Injection Based Induction Regimens in Transplant-Eligible Previously Untreated Multiple Myeloma Patients

...diac and pulmonary disorders, reversible posterior leukoencephalopathy syndrome, gastrointestinal adverse events, thrombocytopenia, neutropenia, tumor lysis syndrome and hepatic events. Women of childbearing potential should avoid becoming pregnant while being treated with VELCADE. Nursing mothers are advi...

Arzerra(TM) (ofatumumab) Demonstrates High Response Rates in Patients With Fludarabine Refractory Chronic Lymphocytic Leukemia (CLL)

...within eight weeks from start of treatment) occurred in four patients (7 percent) in the DR group and two patients (3 percent) in the BFR group. Tumor lysis syndrome was also reported following treatment with ofatumumab. Common adverse reactions (greater than or equal to 10%) were pyrexia, cough, diarrhea,...

Novel VELCADE(R) (Bortezomib) for Injection Based Four-Drug Combination Was Well Tolerated In Previously Untreated Multiple Myeloma Patients

...diac and pulmonary disorders, reversible posterior leukoencephalopathy syndrome, gastrointestinal adverse events, thrombocytopenia, neutropenia, tumor lysis syndrome and hepatic events. Women of childbearing potential should avoid becoming pregnant while being treated with VELCADE. Nursing mothers are advi...

Micromet Presents Data at the 23rd Annual Meeting of the International Society for Biological Therapy of Cancer on the Activity of Human Antibody Adecatumumab against KRAS-mutated Colon Cancer Cells

...te a phase 2 study investigating the activity of adecatumumab in colon cancer patients with completely resected liver metastases." (1) ADCC-mediated lysis of KRAS-mutated Colon Cancer Cells by Anti-EpCAM Antibody Adecatumumab. D. Ruttinger et al. 23rd annual meeting of iSBTc, San Diego Nov. 1st (2008; ab...

Jennerex Completes the Treatment of First Patient Cohort in Phase 1 Trial With Intravenous Delivery of JX-594 Oncolytic Virus

... high levels in cancer cells. To enhance product efficacy, JX-594 is also engineered to express the GM-CSF protein. GM-CSF complements the cancer cell lysis work of the product candidate, leading to a cascade of events resulting in tumor necrosis, tumor vasculature shutdown and an anti-tumoral immune attac...

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Lysis in Biological Technology

Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

Abstract Applied Biosystems has developed new techniques that produce highpurity total RNA from biological sources such as cultured cells. These techniques eliminate the need for post-isolation DNase treatment or other manual handling, and produce a highly pure product, free ...

Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A

Heping Han*, Biren Zhao*, Robert Hsueh # , and Susanne Mumby*, *The Antibody Laboratory and the # Cell Preparation and Analysis Laboratory, The Alliance for Cellular Signaling, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390 ...

Micromet to Present Six Posters on Progress of BiTE Antibodies at the Annual Meeting of American Association for Cancer Research

... Lutterbuese, R. et al. (2009). Highly efficient lysis of KRAS- and BRAF-mutated human colon cancer cells...ake a major contribution to redirected target cell lysis by T cell-engaging BiTE antibody MT110. AACR Annua... Aigner, M. et al. (2009). Effective in vitro lysis of human melanoma cell lines by T lymphocytes redi...

InVitria Among Leading Innovators in Cell Culture Media According to Top Biotechnology Publication

...nes. Also mentioned in the article is InVitria's Lysobac™, a cell lysis reagent used in microbial fermentation to improve recombinant protein yield...ble, well-defined and regulatory compliant cell culture media and bacterial lysis components. They can also contribute to operational efficiency and reduced ...

Proteolix Announces Positive Data From Two Ongoing Phase 2 Clinical Trials of Carfilzomib in Multiple Myeloma at the 50th Annual Meeting of the American Society of Hematology

...ated among relapsed patients and toxicities were manageable. The most common adverse events were fatigue, anemia and neutropenia. Two cases of tumor lysis syndrome were observed among bortezomib-naive patients, and with additional monitoring and management guidelines no further events have been reported....

InVitria Touts Product Advantages at Cambridge's Cell Culture Conference

...actor that also delivers iron similar to transferrin in cell culture media. (see http://www.Lacromin.com ) -- Lysobac - Lysobac is a bacterial cell lysis agent that is more efficient and more consistent compared to chicken lysozyme and provides higher recombinant protein yield compared with mechanical c...

Microchip Biotechnologies, Inc. Awarded Contract Under US Army Commercialization Pilot Program

...will integrate multiple sample processing steps in a small, universal sample preparation module that integrates immunomagnetic separation capture with lysis and nucleic acid purification. "Easy-to-use sample preparation, under field conditions, has always been a major obstacle to deploying back end patho...

Jennerex Completes $8.3 Million Series B Financing and Appoints Dr. Cal Stiller as Director

... high levels in cancer cells. To enhance product efficacy, JX-594 is also engineered to express the GM-CSF protein. GM-CSF complements the cancer cell lysis work of the product candidate, leading to a cascade of events resulting in tumor necrosis, tumor vasculature shutdown and an anti- tumoral immune atta...

InVitria Sponsors Cambridge Healthtech's Optimizing Cell Culture Development Conference

...h promotant as a cell culture ingredient in mammalian cell culture media. (see http://www.Lacromin.com ) -- Lysobac -- Lysobac is a bacterial cell lysis agent that is more efficient and more consistent compared to chicken lysozyme and provides higher protein yield compared with mechanical cell lysis. L...

InVitria Further Strengthens Sales Team with Promotion of Brandy Sargent to Account Manager - Western United States

...dition, Lacromin is a strong growth promotant as a cell culture ingredient in mammalian cell culture media. -- Lysobac - Lysobac is a bacterial cell lysis agent that is more efficient and more consistent compared to chicken lysozyme and provides higher protein yield compared with mechanical cell lysis. L...

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Lysis in Biological Products

Insect Cell Lysis Buffer from BD Biosciences Pharmingen

Description: Insect Cell Lysis Buffer 50 ml...
Company:BD Biosciences Pharmingen

Cell Lysis Buffer (10X) from Cell Signaling Technology

Description: Cell Lysis Buffer is used to lyse cells under nondenaturing conditions....
Company:Cell Signaling Technology

96 well plate shaker, 220 V from Mo Bio Laboratories, Inc.

Description:... Disrupts biological cells for DNA isolation. Designed to hold two 96 well plates (includes 96 well plate adapters from Mo Bio). Provides consistent lysis across the entire plate length (max capacity is 192 individual wells). Can be used for soil, microorganisms, animal tissue, plants, seeds, bones, and...
Company:Mo Bio Laboratories, Inc.

LIGHT DIAGNOSTICS™ CMV pp65 Dextran Leukocyte Separation Kit, included in kit #3247 from CHEMICON

Description:...ended for the qualitative identification of lower matrix protein pp65 of CMV in isolated peripheral blood leukocytes. Kit Components: Erythrocyte lysis Buffer - (Catalog No. 5122) One bottle containing 220 mL of buffered ammonium chloride. (NH4CL) Fixation Solution (5X) - (Catalog No. 5113) One bottl...
Company:CHEMICON

LIGHT DIAGNOSTICS™ CMV pp65 NH4Cl Leukocyte Separation Kit, alternate to #3246 from CHEMICON

Description:... all the materials necessary for the isolation and preparation of Leukocytes for use in CMV antigen studies analysis. Kit Components: Erythrocyte lysis Buffer - (Catalog No. 5122) One bottle containing 220 mL of buffered ammonium chloride. (NH 4 Cl) Fixation Solution (5X) - (Catalog No. 5113) One bot...
Company:CHEMICON

cAMP[125I] Direct SPA Screening Biotrak Assay from GE Healthcare, formerly Amersham Biosciences

Description:... cAMP - [125I] Direct SPA Screening Assay, 5 x 96 wells. SPA.Includes lysis reagents that eliminate lengthy sample extraction procedures and allow direct cAMP measurement (optional use).Range: 1.32-84.28 ng/ml (4-256 pmol/ml)....
Company:GE Healthcare, formerly Amersham Biosciences

PCR Diagnosis-Bacteria Identification Kit from BioChain

Description:... This kit consists of universal lysis buffer, PCR mix, DNA polymerase and universal control primers for 100 25 l ...ccus Aureus, Streptococcus Agalactiae, and etc. With the powerful Universal lysis buffer, the sensitivity of this system is so high that bacteria DNA can be ...
Company:BioChain

One•STrEP™ Protein Interaction Kit from Novagen

Description:...R-IBA 103 Mammalian Expression Vector OneSTrEP Control Vector StrepTag II Antibody HRP Conjugate PP2Ac Goat Polyclonal Antibody 1X StrepTactin lysis Buffer 1X StrepTactin Wash Buffer 1X StrepTactin Elution Buffer with Biotin ...
Company:Novagen

GST Expression and Purification Kit from BD Biosciences Pharmingen

Description:...culovirus Transfer Vector Set pAcGHLT-XylE Baculovirus Control Vector Thrombin Powder Thrombin Dilution Buffer Protease Inhibitor Cocktail Insect Cell lysis Buffer Glutathione Powder GST Elution Buffer PBS Wash Buffer Transfection Buffer...
Company:BD Biosciences Pharmingen

M-PER from Pierce Biotechnology, Inc.

Description:... M-PER Mammalian Protein Extraction Reagent is a liquid, ready-to-use cell lysis solution that means no scraping, no freeze-thaw cycles and no sonication! With M-PER Reagent, rapid lysis occurs in just five minutes at room temperature, yielding 25% more protein ...
Company:Pierce Biotechnology, Inc.

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Lysis in Biological Definition

Lysis

Lysis ( Greek lusis from luein = to separate) is the reduction of symptoms of a disease the dissolving of cells known more specifically as cytolysis osmotic lysis chemical lysis viral lysis a dialogue of Plato about friendship ( philia ), see Lysis (...

Alexander Fleming

... Fleming inspected the Petri dish further and found that the bacterial colonies around the fungus were transparent because their cells were lysing . lysis is the breakdown of cells, and in this case, potentially harmful bacteria. The importance was immediately recognized, however the discovery was still...

Antibody

...ds to various cell receptors and complement proteins. In this way it mediates different physiological effects of antibodies ( opsonization , cell lysis , mast cell, basophil and eosinophil degranulation and other processes). The variable regions of the heavy and light chains can be fused together t...

Antigen

...ex with class I histocompatibility molecules . If cytotoxic CD8 + T cells recognize them, they begin to secrete different toxins that cause the lysis or apoptosis of the infected cell. In order to keep the cytotoxic cells from killing cells just for presenting normal proteins, they run through a ...

Glycolysis

...result in the formation of ATP. These two reactions pull the glycolytic pathway to completion. Etymology From Greek glyk meaning sweet and lysis meaning dissolving. See also Gluconeogenesis Krebs cycle Anaerobic respiration External links The Glycolytic enzymes in G...

Lytic cycle

... 1 Description 1.1 Infection 1.2 Growth 1.3 Replication and lysis Description The lytic cycle is a three-stage process. I...A into DNA, which is then transcribed again into mRNA. Replication and lysis After many virus copies are made, they are assembled into complete viruse...

Peptide

...for a wide array of functions, many of which are not yet understood. Antimicrobial peptides generally disrupt the membranes of a target cell, causing lysis of the cell. How this occurs, and what determines the activity and selectivity of these peptides, is currently only known approximately. It has als...

Phage

...igns of stress (meaning it might be about to die soon), the endogenous phages become active again and start their reproductive cycle, resulting in the lysis of the host cell. An example is phage λ of E. coli . Sometimes, prophages even provide benefit to the host bacterium while they are dormant,...

Peptide

...for a wide array of functions, many of which are not yet understood. Antimicrobial peptides generally disrupt the membranes of a target cell, causing lysis of the cell. How this occurs, and what determines the activity and selectivity of these peptides, is currently only known approximately. It has als...

Virus

...haperones , or proteins made by the host that help the capsid parts come together. The new viruses then leave the cell either by exocytosis or by lysis . Envelope-bound animal viruses instruct the host's endoplasmic reticulum to make certain proteins, called glycoproteins , which then collect in cl...

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Lysis in Biological Dictionary

Lysis

Lysis refers to the death of a cell by breaking of the cellular membrane, causing the contents to spill out, often by viral, enzymic or osmotic mechanisms that compromise its integrity. A fluid containing the contents of... Cytolysis, or osmotic lysis , occurs when a cell bursts due to an...

Minicircles

...rcles ... to probe for the existence of kinks in 94-bp-long minicircles . ... Preparation of mitochondrial telomeric minicircles by alkaline lysis ... To relax supertwisted minicircles , 4 µl of DNA was incubated for 60 min at 15°C ... Minicircles are released from the network into the kin...

Lytic cycle

...y can have either lytic or lysogenic cycle , depending on the environment. In the lytic cycle , l phages replicate rapidly and eventually cause lysis of the ... Definition of lytic at Dictionary.com with free online dictionary, pronunciation, ... lysogenic life cycle . list of lytic viruses...

Gel shift assay

...a gel - shift assay . ... RNA-protein gel mobility shift assay . Munro's binding buffer. 10mM Hepes, pH 7.6. 3 mM MgCl2 ... RNase free H20. lysis buffer. Munro's buffer + 0.2% NP-40 in ... A gel shift assay , also referred to as an EMSA is an electrophoretic mobility assay . ... Gel ...

DNase

...rature fluctuations will ... Thermo Scientific Deoxyribonuclease I ( DNase I) is a single, glycosylated ... DNase I is commonly added to cell lysis reagents to remove the viscosity caused ... ( RQ1 RNase-Free DNase may be used in applications where ... This DNase is suited for applications...

CAT assay

...ific ... A direct assay for chloramphenicol acetyl transferase ( CAT ) has been presented ... Promega, " CAT Enzyme Assay System with Reporter lysis Buffer", on line ... CAT assay . CAT assay . Chloramphenicol Acetyl Transferase ( CAT ) is a bacterial enzyme which inactivates ... GFP Luc...

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