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Lyse in Medical Technology

NanoBio Corporation Presents Preclinical and Interim Clinical Data on Topical Treatments for Herpes Labialis and Onychomycosis at AAD 2008

...without disrupting skin or mucous membranes. Upon contact with the pathogen, the nanodroplets directly interact with the pathogen's outer membranes to lyse the virus, fungus or bacteria. Poster Titles -- Link to posters at http://www.nanobio.com -- Safety, Tolerance, Pharmacokinetics, and Efficacy...

Anadys Reports Preclinical Data Showing ANA773 Promotes Anti-Tumor Activity of Natural Killer Cells

...at includes the secretion of cytokines and the stimulation of NK cells. NK cells mediate anti-tumor activity by multiple mechanisms. NK cells directly lyse tumor cells through the secretion of cytotoxic granules; they promote apoptosis through the production of interferon-gamma (IFN-gamma), and they enhan...

MediGene Announces Cancer-Fighting Virus Shows Promise in Early Clinical Trial

...atic to the liver who fail first-line chemotherapy are sparse. Genetically-engineered oncolytic viruses can selectively replicate, spread in situ, and lyse malignant cells while sparing normal tissue, thus maybe advantageous over chemotherapy. Previous clinical studies have shown that single doses of the ...

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Lyse in Biological Technology

MicroFluidic Systems (MFSI) Receives Patent for Method to Selectively Process Different Biological Cell Types for Analysis.

...re/ -- Microfluidic Systems, a privately-held company, announced today that they received patent number 7,541,166, entitled "Sonication to Selectively lyse Different Cell Types". This method, originally applied to the process of differential extraction of male/female DNA from a mixed forensic sample, has ...

XANTHOCHROMIA IN CEREBROSPINAL FLUID

...aemoglobin in the supernatant fluid, only in vivo haemorrhage results in bilirubin formation. Following a true haemorrhage, red blood cells lyse and release oxyhaemoglobin, which is metabolised to bilirubin. The CSF supernatant after centrifugation is visibly pink or pink-orange due to oxyhae...

QuickLyse Miniprep Kit

... Unlike most protocols that use a 3-step lysis procedure, the QuickLyse Miniprep Kit combines enzymatic and osmotic processes to lyse bacterial cells in a single, 3-minute step. In addition, QuickLyse technology uses fewer buffers, simplifying handling, and savi...

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

... tips, and a combination of trituration and shaking is performed to resuspend the bacterial pellet. Next, wide bore tips are used to add Solution 2 to lyse the bacteria. Again the microplate shaker is activated to assure a homogenous solution. Wide bore tips are then used to transfer the neutralization so...

Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

...her nucleic acid-based assays. Chemistry Applied Biosystems lysis chemistry uses a unique formulation to effectively lyse cells or tissues. The lysis solution is supplied as a 2X formulation and should always be used at a final concentration of 1X. Lysis occurs almost imm...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

...ximately 5.0 x 10 7 viable bacteria/ml (0.4 absorbance) and washed twice in ice-cold PBS (pH 8.0) taking care not to disrupt or lyse cells. The bacteria were resuspended to an absorbance of 0.2 at 525 nm and 1.7 mg of sulfo-NHS-LC-biotin was added per ml of bac...

Genomic DNA Isolation Protocol for 40 l Avian Whole Blood

...lts. 2. Add 40 l whole blood to a 15 ml microfuge tube containing 6 ml cell lysis solution. Quickly pipet up and down 35 times to lyse the cells. Usually no incubation is required; however, if cell clumps are visible after mixing, incubate at 37C until the soluti...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

...ycling was performed on an MJ Research Tetrad. Cycling conditions were as follows: an initial five-minute denaturation at 95C to lyse the cells and release the plasmid DNA, 35 cycles (94C for 1 minute, 58C for 1 minute, 7 2C for 4 minutes), followed by 10 minute...

Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

... and homogenize. Cells grown in a Monolayer: lyse cells by adding 1 ml TRIzol Reagent per 10 cm2 area of cul... 1 x 107 bacterial cells. Resuspend pellet by pipetting to lyse cells. For 102104 cells, add 0.8 ml TRIzol Reagent and 0.2...

Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

... and homogenize. Cells grown in a Monolayer: lyse cells by adding 1 ml TRIzol Reagent per 10 cm2 area of cul... 1 x 107 bacterial cells. Resuspend pellet by pipetting to lyse cells. For 102104 cells, add 0.8 ml TRIzol Reagent and 0.2...
Lyse in Biological Products

Chaps Cell Extract Buffer (10X) from Cell Signaling Technology

Description:... Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommendedfor the preparation of cell lysates to be used with our caspase signaling pathway antibodies...
Company:Cell Signaling Technology

Cell Lysis Buffer (10X) from Cell Signaling Technology

Description:... Cell Lysis Buffer is used to lyse cells under nondenaturing conditions....
Company:Cell Signaling Technology

UltraClean Plant DNA (S) Kit Sample from Mo Bio Laboratories, Inc.

Description:...dy for immediate use. Plant tissue samples are added to a bead beating tube containing beads, bead solution and lysis solution. The prinicipal is to lyse the tissue by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action...
Company:Mo Bio Laboratories, Inc.
Lyse in Biological Definition

Cytotoxic T cell

... which forms pores in the target cell's plasma membrane ; this causes ions and water to flow into the target cell, making it expand and eventually lyse . T C also release granzyme , a serine protease , that can enter target cells via the perforin-formed pore and induce apoptosis (cell death). ...

Phage

...t only specific bacteria. Some phages are virulent , meaning that upon infecting a cell they immediately begin reproducing, and within a short time lyse (destroy) the cell, releasing new phages. (A famous quote from the microbiologist Mark Mller says: Bacteria don't die, they just phage away. ) Some...

Virus

...ane. The virus is then discharged from the cell at these exit sites, referred to as exocytosis. On the other hand, bacteriophages must break open, or lyse , the cell to exit. To do this, the phages have a gene that codes for an enzyme called lysozyme . This enzyme breaks down the cell wall, causing the ...
Lyse in Biological Dictionary

Fusion Protein

...CIENCES-GST fusion protein Handbook (pdf) ... If fusion protein is poorly eluted, try: ... co-purifies with the GST- fusion protein . ... lyse E. coli with B-PER Reagent and rapidly purify the soluble GST- fusion protein . Column kit (10 mg capacity) and spin cup kit (1 mg capacity) A f...
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