Lysates in Medical Technology
Lysates in Medical Products
Biotin-Trapper Streptavidin Magnetic Beads
Description:...r Streptavidin Magnetic Beads (1 mm - 2 mm) are designed for single-step capture of biotinylated molecules such as DNA, RNA or protein from cell lysates
or hybridization reactions....
BcMag Streptavidin Magnetic Beads
Description:...cMag Streptavidin magnetic beads are designed for rapid, single-step capture of biotinylated molecules such as DNA, RNA, antibody or protein from cell lysates
or hybridization reactions. Due to its highly dispersed, it can also be used for cell sorting....
QuantiGene 2.0 RNA Quantification System
Quantitate RNA directly from crude cell lysates
to eliminate amplification bias
Branched DNA technology enhances assay sensitivity to detect low expressing genes
Validate RNAi assays by accura...
Lysates in Medical Dictionary
...Why would your neutrophil level be 19.7? ... In contrast, neutrophil spreading was accelerated when cGMP levels were elevated ... Neutrophil lysates
were immunoprecipitated with mouse anti-FAK (lane 1) or mouse ... Multiple Sclerosis Encyclopaedia - neutrophil ... Once there, the neutrophil ...
... to them are usually... Comparison of the levels in the intracellular lysates
with the virion lysates
on ... About 0.1-0.2% of the intracellular levels of endophilin were reco...
Lysates in Biological News
All eukaryotic kinases share 1 common set of substrates
...e substrates described in the PhosphoBase phosphorylation database. They incubated them with radio actively labelled ATP as a source of phosphate, and lysates
(cell content) of two species of yeast (Candida albicans and Pichia pastoris), a fungus (Fusarium solani), a plant (Triticum aeastivum, wheat), a frui...
Lysates in Biological Technology
Monitoring fluorescence resonance energy transfer (FRET) between GFP
fusions in lysates of the yeast Saccharomyces cerevisiae using the
Monitoring fluorescence resonance energy transfer (FRET) between GFP
fusions in lysates
of the yeast Saccharomyces cerevisiae using the
Paul Gavin # and Mark Prescott # , Ph.D
Daren J. Fyfe, Ph.D*
# Department o...
MaxCyte and Medinet to Present at the 10th International Symposium on Dendritic Cells in Kobe, Japan
...ng process at Medinet's
cell processing centers.
The presentation will discuss how the electroloading of previously
matured DC with whole cell tumor lysates
generates a DC vaccine that has up
to 20-fold enhanced antigen-specific potency compared to traditional DC
vaccines obtained via lysate co-incubation ...
Measuring siRNA-mediated knockdown of IL-8 mRNA
Bypassing many of these variability issues, the QuantiGene assay allows quantification of RNA levels directly from fresh or fixed cell lysates
or tissue homogenates. This technology, based on branched DNA, allows for amplification of the signal, rather than the target, following the cooperati...
cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization
...eparation steps or wash steps. The reagents have been optimized to provide a sensitive, robust and rapid homogeneous assay. Individual assays of cell lysates
or of purified adenylyl cyclase will require optimization and validation based upon the requirements of the individual test systems.
Research Tools for Analysis of ZAP-70
... assay was tested using cell lines as well as purified B and T cells. Table 1 shows that Jurkat (human T cell line) and EL4 (mouse T cell line) cell lysates
gave very good responses while there is no signal with A431 (human fibroblast cell line), Daudi (human B cell line), EB1 (human B cell line) or Ramos ...
Minimizing Photobleaching of Blue Fluorescent Protein (BFP) using
the Varian Cary Eclipse Fluorescence Spectrophotometer
Yeast cells were washed twice in 1 mL MilliQ water before being lysed
using Y-PER (Progen) as per manufacturers instructions. Y-PER lysates
(5 l) were diluted with 1 mL 50 mM Tris/HCl pH 8 and placed in disposable
fluorescence cuvettes (Sarstedt) in the multicell hold...
Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
...ate may be routinely assessed using the Cell Lysate Control Kit, containing lysates
at two concentrations: 104 and 106 cells/well. Total RNA is purified from these two lysates
and compared to RNA standards included in the kit in an ABI PRISM 7700 Sequ...
Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
...tiation, and apoptosis. In this application, human T293 kidney epithelial cells were activated with TGF-β and harvested at different time points. lysates
of these cells were prepared, run on gels, transferred to Hybond membranes, and then blotted according to the protocol previously described. The targe...
Aurum Plasmid 96 Kit Tech Note, Rev A
... time. The protracted sample prep time
for Montage was primarily due to an extremely slow plasmid binding step;
wells containing lysates
from 4 OD culture samples took 16 min to pass
through the plasmid binding plate, as opposed to the 7 min indicated in
Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
Untreated and UV-treated HEK 293 cell lysates
were prepared according
to methods deta... Both untreated HEK 293 and UVtreated HEK 293 cell lysates
at three different protein ...d between UV-treated and untreated cell
varied, the total ERK2 signal level at the same pr...
Lysates in Biological Products
Mouse Anti-Human Clusterin alpha chain Monoclonal Antibody, Unconjugated, Clone 41D from Upstate
Description:...rin purified from human serum Formulation: 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide Quality Assurance: routinely evaluated on RIPA lysates
from HeLa cells or MCF-7 cell lysates. Recognizes clusterin α chain...
Chaps Cell Extract Buffer (10X) from Cell Signaling Technology
Description:... Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommendedfor the preparation of cell lysates
to be used with our caspase signaling pathway antibodies...
Company:Cell Signaling Technology
Calf Liver tRNA from Novagen
Description:... Novagen's Calf Liver tRNA is qualified for use in reticulocyte lysates
for in vitro translation, and is the same supplement used in the Red Nova Lysate ....
Rabbit Reticulocyte Lysate, Untreated from Promega
Description:...ponents and as an abundant source of endogenous globin mRNA. Untreated Lysate is prepared from New Zealand white rabbits in the same manner as treated lysates
with the exception that it is not treated with micrococcal nuclease (1)....
Mouse Anti-PLC delta-1 Monoclonal Antibody, Unconjugated, Clone d1-174 from Upstate
Description:...is-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% Quality Assurance: routinely evaluated by immunoblot on RIPA lysates
from PC-12 cells or bovine brain cytosol preparation Recognizes PLCδ1...
Mouse Anti-NY-ESO-1 Monoclonal Antibody, Unconjugated, Clone E978 from Upstate
Description:...-ESO-1 full-length recombinant protein Formulation: mouse ascites with 0.05% sodium azide Quality Assurance: routinely evaluated by immunoblot on RIPA lysates
from SKMEL-19 cells Recognizes NY-ESO-1...
Rabbit Anti-Human Connexin 36 Monoclonal Antibody,, Clone ZMD.257 from Invitrogen
Description:... antibody Cat. No. 51-6300. Specificity: Reacts with mouse Connexin 36. Reactivity: Mouse (positive control: mouse Connexin 36-transfected HeLa cell lysates
mouse brain retina and pancreas homogenates). Based on sequence homology reactivity with Human rat and zebrafish. Applications: Western blotting ELIS...
Rabbit Anti-PRK2 Polyclonal Antibody, Unconjugated from Upstate
Description:...acids 926-941 (DKKVKPPFIPTIRGRE) of human PRK2. Purification immunoaffinity purified Quality Assurance Routinely evaluated by immunoblot in RIPA lysates
from HeLa cells. Stability 1 year at -20°C from date of shipment...
Rabbit Anti-Human WNT2B / WNT13 Monoclonal Antibody,, Clone ZMD.368 from Invitrogen
Description:...ty: Mouse Human Rat (positive controls: mouse WNT2B/WNT13-pCMV-tag2-transfected 293T Human WNT13A- and WNT13B-pCR3-transfected HEK293 and SK-OV-3 cell lysates
rat ovary homogenates). Applications: Western blotting...
Rabbit Anti-Human PDGF-D Monoclonal Antibody,, Clone ZMD.440 from Invitrogen
Description:...ecificity: Reacts with the ~50 kDa human mouse and rat PDGF-D proteins. Reactivity: Human (positive controls: NTERA-2 (NT2/D1) SK-OV-3 U251 A172 cell lysates
rat ovary mouse testis and mouse fetal brain homogenates). Applications: Western blotting...
Lysates in Biological Dictionary
...expression using RNA interference, a method in which ... siRNA Products ... Chromosome DBase siRNA shRNA Plasmids shRNA Lentiviral Transfected lysates
Flow ... Biotechnology now offers siRNA gene silencers targeted ... Small interfering RNA Mediating RNA interference in cultured mammalian ce...
...eagents. Chemical Precursors ... and amino acids, CAT assay reagents, lipids, and ... Assay for chloramphenicol acetyl transferase in amoebal lysates
... Spot solution onto a 20x20cm 250um silica-gel TLC plate (Baker Si250, Cat #7000-04) ... chloramphenicol acetyltransferase ( CAT Radiometric ...
... Antibodies ... to which the antibody is conjugated; More ... Custom Antibody Production Services. Ig ELISA Quantitation Kits. Immobilized E.coli lysates
... YSK Antibody (C-term) Cell Function, YSK Antibody (C-term) Nuclear Signaling, ... This antibody is generated from rabbits immunized with ...