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Lysates in Medical News

A new light on tumor immunotherapy for gastric cancer

...utic anti-tumor immunity. A research team led by Dr. Liang Wang from China investigated whether bone marrow-derived DCs (BM-DCs) pulsed with tumor lysates induce immunity against gastric cancer. Their results will be published on December 14, 2008 in the World Journal of Gastroenterology . In this st...

IDM Pharma Announces Termination of UVIDEM Development Collaboration and Expected Restructuring in Connection With Evaluation of Strategic Alternatives

...tic specific immunostimulant developed to date by IDM Pharma in partnership with sanofi-aventis. UVIDEM consists of mature dendritic cells loaded with lysates from melanoma tumor cell lines. UVIDEM has been administered to 143 patients in clinical development. About IDM Pharma IDM Pharma is focused on th...

A Mushroom Extract May Impact the Body’s Immune Syste

...to stimulate activation of immune cells; and a final set treated with both the mitogen and mushroom extract, in combination. Separate portions of cell lysates and supernatants (the liquid medium in which the cells were cultured) were collected at 0, 1 and 24 hours, flash-frozen, and stored at –80°C. Samples ...
Lysates in Medical Technology

Monogram Announces Posters to be Presented at the 30th San Antonio Breast Cancer Symposium

... Abstract # 2011: Development of novel proximity-based immunoassays for the detection of HER heterodimerization in breast cancer cell line lysates and formalin-fixed, paraffin-embedded tissue. (Presenter: L. Eli) -- Friday, December 14th, 2007, 7.00 am - 9.00 am CST Abstract # 2012: Ch...

IDM Pharma Announces UVIDEM(R) and EP-2101 Data Presentations at 2007 ASCO Annual Meeting

...ership with sanofi-aventis. Sanofi-aventis has worldwide marketing rights to UVIDEM in melanoma. UVIDEM consists of mature dendritic cells loaded with lysates from melanoma tumor cell lines. UVIDEM is produced in IDM Pharma GMP manufacturing facilities in Irvine, California and in Paris, France. UVIDEM has b...

IDM Pharma Presents Preliminary Results from Phase II UVIDEM Melanoma Vaccine Clinical Trial

...ership with sanofi-aventis. Sanofi-aventis has worldwide marketing rights to UVIDEM in melanoma. UVIDEM consists of mature dendritic cells loaded with lysates from melanoma tumor cell lines. UVIDEM is produced in IDM Pharma GMP manufacturing facilities in Irvine, California and in Paris, France. UVIDEM has b...
Lysates in Medical Products

Biotin-Trapper™ Streptavidin Magnetic Beads

Description:...r™ Streptavidin Magnetic Beads (1 mm - 2 mm) are designed for single-step capture of biotinylated molecules such as DNA, RNA or protein from cell lysates or hybridization reactions....
Company:BioVintage

BcMag Streptavidin Magnetic Beads

Description:...cMag Streptavidin magnetic beads are designed for rapid, single-step capture of biotinylated molecules such as DNA, RNA, antibody or protein from cell lysates or hybridization reactions. Due to its highly dispersed, it can also be used for cell sorting....
Company:Bioclone Inc.

QuantiGene 2.0 RNA Quantification System

Features:... Quantitate RNA directly from crude cell lysates to eliminate amplification bias Branched DNA technology enhances assay sensitivity to detect low expressing genes Validate RNAi assays by accura...
Company:Sigma-Aldrich
Lysates in Medical Dictionary

Neutrophil

...Why would your neutrophil level be 19.7? ... In contrast, neutrophil spreading was accelerated when cGMP levels were elevated ... Neutrophil lysates were immunoprecipitated with mouse anti-FAK (lane 1) or mouse ... Multiple Sclerosis Encyclopaedia - neutrophil ... Once there, the neutrophil ...

Intracellular

... to them are usually... Comparison of the levels in the intracellular lysates with the virion lysates on ... About 0.1-0.2% of the intracellular levels of endophilin were reco...
Lysates in Biological News

All eukaryotic kinases share 1 common set of substrates

...e substrates described in the PhosphoBase phosphorylation database. They incubated them with radio actively labelled ATP as a source of phosphate, and lysates (cell content) of two species of yeast (Candida albicans and Pichia pastoris), a fungus (Fusarium solani), a plant (Triticum aeastivum, wheat), a frui...
Lysates in Biological Technology

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse Paul Gavin # and Mark Prescott # , Ph.D Daren J. Fyfe, Ph.D* # Department o...

MaxCyte and Medinet to Present at the 10th International Symposium on Dendritic Cells in Kobe, Japan

...ng process at Medinet's cell processing centers. The presentation will discuss how the electroloading of previously matured DC with whole cell tumor lysates generates a DC vaccine that has up to 20-fold enhanced antigen-specific potency compared to traditional DC vaccines obtained via lysate co-incubation ...

Measuring siRNA-mediated knockdown of IL-8 mRNA

...principle Bypassing many of these variability issues, the QuantiGene assay allows quantification of RNA levels directly from fresh or fixed cell lysates or tissue homogenates. This technology, based on branched DNA, allows for amplification of the signal, rather than the target, following the cooperati...

cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization

...eparation steps or wash steps. The reagents have been optimized to provide a sensitive, robust and rapid homogeneous assay. Individual assays of cell lysates or of purified adenylyl cyclase will require optimization and validation based upon the requirements of the individual test systems. K...

Research Tools for Analysis of ZAP-70

... assay was tested using cell lines as well as purified B and T cells. Table 1 shows that Jurkat (human T cell line) and EL4 (mouse T cell line) cell lysates gave very good responses while there is no signal with A431 (human fibroblast cell line), Daudi (human B cell line), EB1 (human B cell line) or Ramos ...

Minimizing Photobleaching of Blue Fluorescent Protein (BFP) using the Varian Cary Eclipse Fluorescence Spectrophotometer

... Yeast cells were washed twice in 1 mL MilliQ water before being lysed using Y-PER (Progen) as per manufacturers instructions. Y-PER lysates (5 l) were diluted with 1 mL 50 mM Tris/HCl pH 8 and placed in disposable fluorescence cuvettes (Sarstedt) in the multicell hold...

Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

...ate may be routinely assessed using the Cell Lysate Control Kit, containing lysates at two concentrations: 104 and 106 cells/well. Total RNA is purified from these two lysates and compared to RNA standards included in the kit in an ABI PRISM 7700 Sequ...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

...tiation, and apoptosis. In this application, human T293 kidney epithelial cells were activated with TGF-β and harvested at different time points. lysates of these cells were prepared, run on gels, transferred to Hybond membranes, and then blotted according to the protocol previously described. The targe...

Aurum Plasmid 96 Kit Tech Note, Rev A

... time. The protracted sample prep time for Montage was primarily due to an extremely slow plasmid binding step; wells containing lysates from 4 OD culture samples took 16 min to pass through the plasmid binding plate, as opposed to the 7 min indicated in the manual...

Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays

... ) Untreated and UV-treated HEK 293 cell lysates were prepared according to methods deta... Both untreated HEK 293 and UVtreated HEK 293 cell lysates were tested at three different protein ...d between UV-treated and untreated cell lysates varied, the total ERK2 signal level at the same pr...
Lysates in Biological Products

Mouse Anti-Human Clusterin alpha chain Monoclonal Antibody, Unconjugated, Clone 41D from Upstate

Description:...rin purified from human serum Formulation: 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide Quality Assurance: routinely evaluated on RIPA lysates from HeLa cells or MCF-7 cell lysates. Recognizes clusterin α chain...
Company:Upstate

Chaps Cell Extract Buffer (10X) from Cell Signaling Technology

Description:... Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommendedfor the preparation of cell lysates to be used with our caspase signaling pathway antibodies...
Company:Cell Signaling Technology

Calf Liver tRNA from Novagen

Description:... Novagen's Calf Liver tRNA is qualified for use in reticulocyte lysates for in vitro translation, and is the same supplement used in the Red Nova Lysate ....
Company:Novagen

Rabbit Reticulocyte Lysate, Untreated from Promega

Description:...ponents and as an abundant source of endogenous globin mRNA. Untreated Lysate is prepared from New Zealand white rabbits in the same manner as treated lysates with the exception that it is not treated with micrococcal nuclease (1)....
Company:Promega

Mouse Anti-PLC delta-1 Monoclonal Antibody, Unconjugated, Clone d1-174 from Upstate

Description:...is-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% Quality Assurance: routinely evaluated by immunoblot on RIPA lysates from PC-12 cells or bovine brain cytosol preparation Recognizes PLCδ1...
Company:Upstate

Mouse Anti-NY-ESO-1 Monoclonal Antibody, Unconjugated, Clone E978 from Upstate

Description:...-ESO-1 full-length recombinant protein Formulation: mouse ascites with 0.05% sodium azide Quality Assurance: routinely evaluated by immunoblot on RIPA lysates from SKMEL-19 cells Recognizes NY-ESO-1...
Company:Upstate

Rabbit Anti-Human Connexin 36 Monoclonal Antibody,, Clone ZMD.257 from Invitrogen

Description:... antibody Cat. No. 51-6300. Specificity: Reacts with mouse Connexin 36. Reactivity: Mouse (positive control: mouse Connexin 36-transfected HeLa cell lysates mouse brain retina and pancreas homogenates). Based on sequence homology reactivity with Human rat and zebrafish. Applications: Western blotting ELIS...
Company:Invitrogen

Rabbit Anti-PRK2 Polyclonal Antibody, Unconjugated from Upstate

Description:...acids 926-941 (DKKVKPPFIPTIRGRE) of human PRK2. Purification immunoaffinity purified Quality Assurance Routinely evaluated by immunoblot in RIPA lysates from HeLa cells. Stability 1 year at -20°C from date of shipment...
Company:Upstate

Rabbit Anti-Human WNT2B / WNT13 Monoclonal Antibody,, Clone ZMD.368 from Invitrogen

Description:...ty: Mouse Human Rat (positive controls: mouse WNT2B/WNT13-pCMV-tag2-transfected 293T Human WNT13A- and WNT13B-pCR3-transfected HEK293 and SK-OV-3 cell lysates rat ovary homogenates). Applications: Western blotting...
Company:Invitrogen

Rabbit Anti-Human PDGF-D Monoclonal Antibody,, Clone ZMD.440 from Invitrogen

Description:...ecificity: Reacts with the ~50 kDa human mouse and rat PDGF-D proteins. Reactivity: Human (positive controls: NTERA-2 (NT2/D1) SK-OV-3 U251 A172 cell lysates rat ovary mouse testis and mouse fetal brain homogenates). Applications: Western blotting...
Company:Invitrogen
Lysates in Biological Dictionary

SiRNA

...expression using RNA interference, a method in which ... siRNA Products ... Chromosome DBase siRNA shRNA Plasmids shRNA Lentiviral Transfected lysates Flow ... Biotechnology now offers siRNA gene silencers targeted ... Small interfering RNA Mediating RNA interference in cultured mammalian ce...

CAT assay

...eagents. Chemical Precursors ... and amino acids, CAT assay reagents, lipids, and ... Assay for chloramphenicol acetyl transferase in amoebal lysates ... Spot solution onto a 20x20cm 250um silica-gel TLC plate (Baker Si250, Cat #7000-04) ... chloramphenicol acetyltransferase ( CAT Radiometric ...

Antibody

... Antibodies ... to which the antibody is conjugated; More ... Custom Antibody Production Services. Ig ELISA Quantitation Kits. Immobilized E.coli lysates ... YSK Antibody (C-term) Cell Function, YSK Antibody (C-term) Nuclear Signaling, ... This antibody is generated from rabbits immunized with ...
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(Date:9/22/2014)... Steven Reinberg HealthDay Reporter ... that bacterial pneumonia can raise your risk of heart problems, ... and kill heart cells, increasing the chances of heart failure, ... report. In mice, monkeys and human heart tissue, researchers ... and they tried a vaccine that might one day prevent ...
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(Date:9/22/2014)... MAINE AND BOSTON -- In a collaboration that ... advance cancer research and patient care and accelerate ... Beth Israel Deaconess Medical Center (BIDMC) have established ... institutions, presidents announced today. , A recently signed ... Center to establish a comprehensive relationship in the ...
(Date:9/22/2014)... NCPDP, the not-for-profit pharmacy standards development ... 2014 Educational Summit , “Improving the Infrastructure for ... 11, 2014 at the Hyatt Regency Indianapolis. Steven ... Technology, Office of the National Coordinator for Health ... takes place right before NCPDP’s Work Group Meetings, ...
Breaking Medicine News(10 mins):Health News:Scientists Spot How Bacterial Pneumonia Damages the Heart 2Health News:Scientists Spot How Bacterial Pneumonia Damages the Heart 3Health News:At 2014 Clinton Global Initiative Annual Meeting, Seafood Nutrition Partnership Announces Pilot Program to Reduce Women’s Heart Disease Risks 2Health News:At 2014 Clinton Global Initiative Annual Meeting, Seafood Nutrition Partnership Announces Pilot Program to Reduce Women’s Heart Disease Risks 3Health News:At 2014 Clinton Global Initiative Annual Meeting, Seafood Nutrition Partnership Announces Pilot Program to Reduce Women’s Heart Disease Risks 4Health News:TweetMyJobs and HealthcareSource Partner To Provide Social Recruiting Solutions to Healthcare Organizations 2Health News:TweetMyJobs and HealthcareSource Partner To Provide Social Recruiting Solutions to Healthcare Organizations 3Health News:TweetMyJobs and HealthcareSource Partner To Provide Social Recruiting Solutions to Healthcare Organizations 4Health News:The Jackson Laboratory and BIDMC announce multifaceted affiliation 2Health News:The Jackson Laboratory and BIDMC announce multifaceted affiliation 3Health News:NCPDP Educational Summit, Improving the Infrastructure for Continuity of Care, Features Keynote, Steven Posnack 2Health News:NCPDP Educational Summit, Improving the Infrastructure for Continuity of Care, Features Keynote, Steven Posnack 3
(Date:9/19/2014)... Conn. , Sept. 19, 2014 Nxt-ID, Inc. (Nasdaq: ... biometric authentication company focused on the growing m-commerce market, updates the ... world champion in the lightweight and light middleweight weight classes, ... Yesterday it was announced that Game of Thrones actor ... Married with Children star Katey Sagal have ...
(Date:9/18/2014)... circuit located deep in the primitive brainstem has revealed ... at Harvard School of Medicine and the University at ... only the second "sleep node" identified in the mammalian ... sufficient to produce deep sleep. , Published online in ... that fully half of all of the brain,s sleep-promoting ...
(Date:9/18/2014)... A team of researchers from McGill University and ... form of polyethylene ,microbeads, Canadian Journal of ... likely originate from cosmetics, household cleansers, or industrial ... abrasives. Owing to their small size and buoyancy, ... Microplastics are a global contaminant in the ...
Breaking Biology News(10 mins):NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 2NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 3No sedative necessary: Scientists discover new 'sleep node' in the brain 2Microplastic pollution discovered in St. Lawrence River sediments 2
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