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Luminometer in Biological News

Altered gene can increase risk of schizophrenia

...hus, the amount of expression of each SNP would be reflected (via the regulatory DNA) in the intensity of the light produced. An instrument known as a luminometer measured the glow produced and showed a dramatic increase in gene expression in one variant over the others. These results echo the increased expressi...
Luminometer in Biological Technology

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

Evelyn McGown, Ph.D. , Michael Su, M.S. and Jin Liao, M.D., Ph.D. Molecular Devices Corporation, 8/00 INTRODUCTION Luciferase from the North American firefly (Photinus pyralis) is very popular foruse in Reporter Gene Assays. The enzyme catalyzes the AT...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 INTRODUCTION Reporter gene assays are used to study eukaryotic gene expression. Dual geneticreporters are commonly used in transient transfections of cultured cells tominimiz...

High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 INTRODUCTION Immunoassays have traditionally employed colorimetric indicators. As such, multiple dilutions of samples were necessary to ensure that they fell within the dynam...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 INTRODUCTION DNA measurements are important for many aspects of molecular biology. Of thespectrophotometric methods available, UV absorbance measurement at 260 nm isthe simpl...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 INTRODUCTION Adenosine triphosphate (ATP) is present in all living cells. Because the level isstrictly-controlled, assay of ATP can be used as a indicator of viable cell numb...

Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)

Jinfang Liao, M.D., Ph.D. and Evelyn McGown, Ph.D. Molecular Devices Corporation, 8/00 INTRODUCTION The photoprotein aequorin, a bioluminescent protein from the coelentratejellyfish Aequorea victorea, has proven to be highly sensitive to the trace amou...

Measuring siRNA-mediated knockdown of IL-8 mRNA

...pression knockdown studies is the validation of expression inhibition. The QuantiGene Reagent System and the Molecular Devices LMax II 384 microplate luminometer were used to measure IL-8 mRNA in PMA-induced HeLa cells, following siRNA treatment. Quantitative assessment of the knockdown discerned differences of...

Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening

... be measured at low throughput using a single-tube luminometer but until recently could not be considered for HTS because luminometer dispenser designs make it impossible to inject a d...ry to fill the dead volume of the injectors of the luminometer is usually between 1.5 mL (single injector devices...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

... to the manufacturers specifications (Galacto-Light Plus, Tropix, Inc.). Enzyme activity was measured using a LumiCount microplate luminometer (Packard Instrument Company). QUANTITATIVE PCR Quantitative PCR* (Heid et al. 1996) was used as an alternative ...

Transfection Reagent Selector Kit Handbook

... these components are mixed with cell lysates containing luciferase, a flash of light is emitted. Light signals are detected using a luminometer or a liquid scintillation counter. -Galactosidase The prokaryotic enzyme b-galactosidase can be assayed colo...

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Luminometer in Biological Products

Thermal Printer Paper for Monolight Luminometer 1 each from BD Biosciences Pharmingen

Description: Thermal Printer Paper for Monolight(tm) Luminometer 1 each...
Company:BD Biosciences Pharmingen

BD Monolight 3096 Microplate Luminometer from BD Biosciences Pharmingen

Description: The MONOLIGHT 3096 Microplate Luminometer has been designed for the detection of chemi- and bioluminescence and for all measurements of glow and flash luminescence on microplates, i.e. for reactions where the light remain nearly constant over a longer period of time, as well as for very fas...
Company:BD Biosciences Pharmingen

Lumat Singel Tube Luminometer LB 9507 from BERTHOLD TECHNOLOGIES GmbH & Co. KG

Description: The Lumat tube luminometer provides the sensitivity and versatility you expect from a general purpose luminometer. Advanced digital photon counting with selected photomultipliers ensure superior sensitivity and best match of spectral demands. Equipped with up to 2 reagent injectors the Lumat is ide...
Company:BERTHOLD TECHNOLOGIES GmbH & Co. KG

Luciferase Luminescent Assay Kit, LucLite from PerkinElmer

Description:... system produces a long lived glow type signal with a half-life of several hours, which makes it ideal for use on the PerkinElmer LumiCount Microplate luminometer or the PerkinElmer TopCount Microplate Scintillation and Luminescence Counter. When performing high-throughput assays using luclite and the TopCount, ...
Company:PerkinElmer
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