Luciferase

Farnesoid X receptor regulates cystathionase

.... Barbara Renga and her colleagues in the University of Perugia, Italy used luciferase transfection assay, Electrophoretic Mobility Shift Assay (EMSA), Chromatin ...ontaining a potential IR1 binding site. Co-transfection of HepG2 cells with luciferase reporter vector containing four copies of this putative IR1 resulted in tra...

Master molecular switch may prevent the spread of cancer cells to distant sites in the body

...each well of the plate expresses only one individual gene product. The Carstens lab developed a splicing "reporter" that makes cells express a firefly luciferase gene and "glow" when it is spliced in the epithelial cell pattern. Cells with this reporter were individually placed over wells containing each cDNA a...

What are protective effects of anti-ricin A-chain aptamer?

... RTA, against ricin-induced toxicity in cell-based luciferase translation and cell cytotoxicity assays were eval...n protect against ricin ribotoxicity in cell-based luciferase and cell cytotoxicity assays. Hence, RNA aptamer ... this report, authors utilized a stable cell-based luciferase assay and showed that 31RA aptamer also neutralize...

Monogram Biosciences Announces Immediate Availability of Enhanced Sensitivity Trofile(TM) HIV Tropism Assay

...on. Virus replication results in the production of luciferase from a luciferase gene that is carried into the cell by the virus. The production of luciferase in either CCR5 cells, CXCR4 cells or both cell typ...

Firefly genes allow testing of new therapy against lymphoma

...netically inserted it into these tumor cells. That gene produces the enzyme luciferase in the insects which, when combined with another compound, luciferin, cause...esearchers injected luciferin into the animals. Cancer cells containing the luciferase would combine with the luciferin and glow in the dark, giving the team a cl...

PET and bioluminescent imaging aid evaluation of stem cells' potential for new ways to treat disease

...nce there are many things that we dont know about stem cell biology, noted Lee. For this study, researchers used a fusion protein combining firefly luciferase (a light-emitting substance) for optical imaging, a red fluorescent protein for cell separation and a virus enzyme thymidine kinase for PET imaging in...

Monogram Announces First Medicare Payment for Trofile(TM)

...on. Virus replication results in the production of luciferase from a luciferase gene that is carried into the cell by the virus The production of luciferase in either CCR5 cells, CXCR4 cells or both cell typ...

A glowing energy measure for heart cells

... In order to study the working of a heart cell mitochondria, the researchers used the protein; luciferase, usually present in the tails of fireflies. luciferase glows in the presence of ATP. Thus, the intensity of light serves as a meter to measure the amount of ATPs generated by the mitochondria. "Exact...

Luciferase in Biological News

Fox Chase researchers uncover one force behind the MYC oncogene in many cancers

...e, Testa and Jinfei Xu, PhD, a research associate in the laboratory, used a luciferase assay developed from the luciferase enzyme fireflies use to make light to see exactly where DLX5 protein binds...

Single adult stem cell can self renew, repair tissue damage in live mammal

...f the transplanted MuSC "took." The scientists, Alessandra Sacco and Helen Blau, had genetically engineered the transplanted MusSC to express Pax7 and luciferase proteins. As a result, every transplanted cell glowed under ultraviolet light and was easy to trace. "To be able to detect the presence of the c...

Disrupting common parasites' ability to 'talk' to each other reduces infection

...screened for low toxicity to animals. Scientists tested one of those herbicides against toxoplasmosis, labeling the test parasites with the firefly luciferase protein. Whole animal imaging showed that treatment with the herbicide reduced the number of parasites in infected mice during the initial infection a...

Firefly protein lets researchers monitor molecule linked to cancer

...atory has previously developed techniques that use luciferase to monitor protein-protein interactions. Researche...amera to take real-time measurements of light from luciferase in cell cultures and in cells within live animals...., Gross altered cell lines to genetically fuse the luciferase protein to IKB (IKappaB), the protein that comes i...

Newly discovered genetic disease sheds light on body's water balance

...ethod to determine cAMP levels in cells by using a luciferase plasmid that responds to cAMP. luciferase is the substance that lights up in fireflies. Feld...t wild-type cells contain low levels of light from luciferase -- and thus low levels of cAMP. In Feldman's ex...

Glow-in-the-dark zebrafish at UH hold keys to biological clocks

...ly occurring clock-regulated gene. The protein that it encodes is produced at highest levels near dawn, and when the luc gene is inserted into it, the luciferase protein is produced in a similar way. The result is that these fish glow rhythmically, emitting more light during the day than during the night. The a...

UCLA scientists transform HIV into cancer-seeking missile

...nchecked." In order to track the carrier's journey, the scientists added luciferase ?the protein that makes fireflies glow ?to the AIDS virus. They injected th...d," said Chen. When the researchers held the mouse under the camera, the luciferase illuminated the cancer cells, which glowed through the animal's bones, musc...

Luciferase in Biological Technology

Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar

Tracy Worzella and Brad Larson Promega Corporation, Madison, WI, USA Application Note 139 Rev. 7/2006 Multiplexing homogeneous cell-based assays for analysis of different ...

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

Evelyn McGown, Ph.D. , Michael Su, M.S. and Jin Liao, M.D., Ph.D. Molecular Devices Corporation, 8/00 INTRODUCTION Luciferase from the North American firefly (Photinus pyralis) is very popular foruse in Reporter Gene Assays. The enzyme catalyzes the AT...

Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators

Stable reporter cell lines for signal transduction pathway readout Lisa Hexdall Li Xu Alan Greener Mary Buchanan John Bauer Chao-Feng Zheng Stratagene Stratagene now offers the PathDetect HLR-CREB, HLR-Elk1, and HLR-cJun, cell lines F ...

Monogram Biosciences Launches First Resistance Assay for HIV Integrase Inhibitors

...tein. Completion of a single replication cycle results in the production of luciferase activity in infected cells. Infection in the presence of drug is performed ...s is sensitive or resistant to integrase inhibitors. Based on the amount of luciferase activity produced in the absence of drug, PhenoSense Integrase also provide...

Sensitivity Enhancements to Monogram's Trofile(TM) Assay Demonstrate Improved Power to Select Patients for CCR5 Antagonist Therapy

...plication. Virus replication results in the production of luciferase from a luciferase gene that is carried into the cell by the virus. The production of luciferase in either CCR5 cells, CXCR4 cells or both cell types defines the co-recepto...

Increased Light Leads to Early Detection: Caliper Life Sciences Introduces Bioware(R) Ultra Cancer Cell Lines

...ses sooner. Caliper's Bioware Ultra light producing cell lines incorporate the enhanced luc2 gene from Promega. The luc2 gene, an optimized version of luciferase for expression in mammalian cells, creates intensified visible light allowing more sensitive non-invasive detection of cells in vivo. Bioware Ultra ce...

Data Presented on Monogram HIV Tropism Tests at Retrovirus Conference sets New Standards of Assay Sensitivity

...on. Virus replication results in the production of luciferase from a luciferase gene that is carried into the cell by the virus. The production of luciferase in either CCR5 cells, CXCR4 cells or both cell typ...

Study Reaffirms Superiority of Trofile(TM) Assay

...on. Virus replication results in the production of luciferase from a luciferase gene that is carried into the cell by the virus. The production of luciferase in either CCR5 cells, CXCR4 cells or both cell typ...

Near Infrared Technology and Optical Agents For Molecular Imaging

...ect technique based on the expression of firefly luciferase from recombinant plasmids inserted into hybrid c...light in the target cells. In a second case, the luciferase gene can be activated by chemical induction of t... and analysis is limited to cells expressing the luciferase gene. Thus it is not translatable to clinical pr...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

...measured in a single reaction mixture. The firefly luciferase is typicallyused as the experimental reporter, and the renilla luciferase serves as the control. Figure 1 illustrates the two enzymatic reactions. Firefly luciferase enzymecatalyzes the oxidation of luciferin with th...

Luciferase in Biological Products

pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation

Description: The potential applications for the use of small interfering RNA (siRNA) molecules to silence gene expression via the RNA interference (RNAi) pathway have generated tremendous interest in the cellular and molecular biology fields. siRNA for experimental use can be synthesized in vitro, either chemic...
Company:Mirus Bio Corporation

pMIR-mU6-Luc, Mouse U6 Luciferase Control Vector from Mirus Bio Corporation

Description: The potential applications for the use of small interfering RNA (siRNA) molecules to silence gene expression via the RNA interference (RNAi) pathway have generated tremendous interest in the cellular and molecular biology fields. siRNA for experimental use can be synthesized in vitro, either chemic...
Company:Mirus Bio Corporation

luciferase Assay Kit, SuperLight from BioAssay Systems

Description:... 2-min, single-step add-mix-measure assay for luciferase expression and HTS in mammalian cells. Linear detection range: 2 fg to 46 ng luciferase....
Company:BioAssay Systems

Luciferase Reporter Assay Kit from MBL International

Description:... one of the several main MAP kinase groups Firefly luciferase has been used as a sensitive reporter for studying gene regulation and function. luciferase provides a 1000-fold increase in sensitivity in co...ramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin...
Company:MBL International

ENGENISTM siRNA Test System from Yorkshire Bioscience Ltd

Description:...creening a large number of potential siRNA target sequences in simple transient transfection experiments, measuring the reduction in reporter (firefly luciferase from Photinus pyralis) gene expression....
Company:Yorkshire Bioscience Ltd

Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

Description:...the PPi liberated by the activities of acetyl-CoA synthetase (Figure 3) and luciferase (Figure 4). In these experiments, we have detected as few as 5 10-5 U of acetyl-CoA synthetase activity and 10-2 U of luciferase activity....
Company:Molecular Probes (Invitrogen)

Luciferase Luminescent Assay Kit, LucLite from PerkinElmer

Description:... valuable technique in the development of reporter gene assays in which the luciferase is used as an indicator for the transcription of other genes. The choice of the luciferase enzyme is due to advantages such as high sensitivity and relatively simple ...
Company:PerkinElmer

ATP contamination testing from Mo Bio Laboratories, Inc.

Description:... Certify products ATP-Free using Mo Bio luciferase assay. Mo Bio Laboratories offers the most sensitive ATP-Free certification service that will allow you to certify your products ATP-Free with a high...
Company:Mo Bio Laboratories, Inc.

More>>

Luciferase in Biological Dictionary

Polysome

...o acid supply. ... Polysome preparation from worm extracts containing luciferase construct (2/22 ... Notes: This polysome prep separated the background luciferase in the extract ... The free polysome pellets were dissolved in ... Po...

Monocistronic

...ed to the first, how could ... 5 -UTR containing monocistronic reporter mRNAs continue ... monocistronic analysis of RNAs in vitro, the firefly luciferase ... or uncapped monocistronic ... GATA-4 5'-UTR sequences confer efficient translation of uncapped monocistronic reporter mRNAs. A, SP6-based...

Fusion Protein

...ecific CTL responses and protect mice ... Immunohistochemical detection of His•Tag fusion proteins in transfected COS-1 cells ... Tag firefly luciferase (Fluc) fusion protein was transiently transfected into ... Affinity purification of GST fusion proteins ... PopCulture extraction and GST•T...

CAT assay

...sis Buffer", on line ... CAT assay . CAT assay . Chloramphenicol Acetyl Transferase ( CAT ) is a bacterial enzyme which inactivates ... GFP luciferase assay . Other Resources. PubMed Google ... Radiochemicals - NEN Brand Radiochemicals from ... CAT Assay Reagents. Chemical Precursors ... and ...