Rapid DNA Ligation Kit from Roche Applied Science
Description:The Rapid DNA Ligation Kit enables ligation of sticky-end or blunt-end DNA fragments in just 5 min at 15-25 C. Depending on the DNA concentration, either circular (low DNA concentration) or concatemeric (high DNA concentration) ligation products are formed....Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
Choose Shrimp Alkaline Phosphatasefor Easier Heat Inactivation Simplify dephosphorylation of 5′ ends on DNA vectors byusing our high-quality Shrimp Alkaline Phosphatase.Avoid phenol:chloroform extractions; after dephosphorylat...Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
The efficiency of the Rapid DNA Ligation Kit fromRoche Molecular Biochemicals was compared withstandard and optimized conventional ligation procedures.The reactions generated circular recombinantbacteriophage DN...High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures
Paul Zoller, Research and Development Department, Genetic Systems Division, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 It has become clear that electroporation of E. coli results in significantly higher transformation efficiencies than chemical transformation procedur...