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Ligase in Medical News

Penn scientists find a protein that inhibits Ebola from reaching out to infect neighboring cells

...f Ebola virus VP40 VLPs. Addressing the molecular mechanism of this inhibition, the researchers demonstrated that ISG15 interacts with Nedd4 ubiquitin ligase and inhibits ubiquitination of VP40, thus blocking budding of VP40 VLPs. These data provide evidence of antiviral activity of ISG15 against Ebola viru...

What is the more suitable for early detection of low abundant lamivudine-resistant mutants?

... and time required, exist in these methods. Real-time PCR is able to quantitatively detect a small portion of resistant mutants in HBV populations and ligase detection reaction (LDR) is a newly developed method for detection of low abundant mutants in the background of wild-type HBV. However, there are no s...

JCI table of contents: Nov. 8, 2007

...scle damage and that inhibiting atrogin-1 function might protect against this unwanted side effect of statins. TITLE: The muscle-specific ubiquitin ligase atrogin-1/MAFbx mediates statin-induced muscle toxicity AUTHOR CONTACT: Stewart H. Lecker, Beth Israel Deaconess Medical Center, Boston, Massachu...

Advances in drug screening: building a better haystack for the needles of tomorrow

...icine, The James Birrell and Lilah Funds for Neuroblastoma Research, and the Sick Kids Foundation. Identification of inhibitors for MDM2 ubiquitin ligase activity from natural products by a novel high throughput electrochemiluminescent screen: Abstract C 55. Scientists laboring intensively to develo...

Understanding HIV Interference With Infected Cell Division

...r induce their degradation. The researchers demonstrated that association of Vpr with this ubiquitinating complex, also called an E3 ubiquitin ligase complex, is essential for the defect in cell division induced by Vpr. Further characterization of this protein complex as well as the elucidation of t...

Protein Targets for Carcinogenesis

...nzymes is needed to attach Ub to another protein: E1, E2 and E3. E1 activates Ub and transfers it to E2, which in turn cooperates with an so-called Ub ligase (E3) that couples Ub to a specific target protein. However, Dikic and colleagues discovered that certain proteins can be ubiquitinated independently o...

Role Of Estrogen In Breast-Cancer

...ins fell to 20 percent of normal, the level of N-CoR's messenger RNA went untouched. The reduction of N-CoR followed an up regulation of the ubiquitin ligase Siah2, an enzyme that targets certain proteins for degradation. This "secondary effect" may have broad implications for other important cellular a...
Ligase in Medical Products

Agarose NA

Description:...h low electroendosmosis (EEO) is recommended for most DNA/RNA applications. If separated nucleic acids are to be used as substrates for enzymes, e.g., ligase and restriction endonucleases, a special highly-purified agarose such as Agarose NA should be used....
Company:GE Healthcare, formerly Amersham Biosciences
Ligase in Biological News

Muscle atrophy through thick but not thin

...ntaining thin filaments. During atrophy, this structure is disassembled, but exactly how was not known. MuRF1, an atrophy-related gene, is a ubiquitin ligase that "ubiquitylates," or tags a protein, by attaching a ubiquitin molecule, marking it for degradation by the cell. It was unclear when and how ubiqui...

Lipid droplets lead a Spartin existence

...knocking-down the protein caused an increase in both the organelle's size and number. In turn, Spartin is regulated by ubiquitination by the ubiquitin ligase WWP1. Overexpressed WWP1 removed Spartin from LDs and promoted its degradation. A truncated form of Spartin found in a rare neuronal disease called...

New research helps explain genetics of Parkinson's disease

...articles will be published online Monday, November 24 ( www.jcb.org ). Loss-of-function mutations in the gene Park2, which encodes an E3 ubiquitin ligase (Parkin), are implicated in half the cases of recessive familial early-onset Parkinson's disease. Several lines of evidence suggest that Parkin loss i...

Death, division or cancer? Newly discovered checkpoint process holds the line in cell division

... Yen, along with visiting researcher Avram Hershko, Ph.D., of Technion, discovered the ability of MCF2 to block mitosis by shutting down an ubiquitin ligase enzyme known as the anaphase-promoting complex/cyclosome (APC/C). Hershko was awarded the 2004 Nobel Prize for Chemistry along with former Fox Chase r...

MIT probe may help untangle cells' signaling pathways

...oteins interact, the enzyme and substrate also interact, which can be easily detected. To create the probes, the researchers used the enzyme biotin ligase and its target, a 12-amino-acid peptide. Their work is conceptually related to an approach that uses GFPs (green fluorescent proteins), which glow ...

Purple extremist thrives under inhospitable conditions

... hot or cold, , require mild environmental conditions. "The Ferroplasma DNA ligase is unique," states Olga Golyshina: "It actually requires extremely acidic c...n, but require magnesium or potassium to function. Extraordinarily, the DNA ligase of Ferroplasma contains iron and does not need either magnesium or potassiu...

RING finger protein 5 may guide treatment for muscle disease in older adults

... identify and remove those proteins that are not properly folded. Ubiquitin ligase RNF5 (or RING Finger Protein 5) acts much like one of these quality-control...ains, still need to be addressed. We know the substrates for this ubiquitin ligase in C. elegans, but not yet in human muscle. ...

RNA enzyme structure offers a glimpse into the origins of life

...een two RNA subunits. Robertson obtained the ligase ribozyme through a kind of test-tube evolution whe...ratch. In the Ellington lab, Robertson evolved the ligase ribozyme (called the L1 ligase) and determined whi...ly occuring enzymes, Robertson said. "The L1 ligase appears to use strategies of transition-state stab...

Plant protection from cold decoded

...ine version of the Proceedings of the National Academy of Sciences in a paper titled The Negative Regulator of Plant Cold Responses, HOS1 is a RING E3 ligase That Mediates the Ubiquitation and Degredation of ICE1. Zhu co-authored the paper with UCR colleagues Chun Hai Dong and Manu Agarwal; and Yiyue Zhang ...

Telling axons where to go - and grow

...quitin/proteosome protein degradation pathway in the regulation of local actin dynamics in neurons. The authors are able to show that the ubiquitin ligase Rnf6 polyubiquitinates the kinase LIMK1, targeting it for proteosomal degradation in the growth cones of hippocampal neurons. LIMK1 regulates the dyn...
Ligase in Biological Technology

World Gene Amplification Technologies Market to Reach $2.2 Billion by 2015, According to New Report by Global Industry Analysts, Inc.

...h in the US, Europe, and Japan. Some of the alternative technologies existing in the market include OmniPlex, Strand Displacement Amplification (SDA), ligase Chain Reaction (LCR), and Hybrid Capture technology. Gene amplification technology plays a vital role in the diagnostics field. With increasing ag...

High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures

...ligation reactions consisted of DNA, 1x ligase buffer (50 mM Tris, pH 7.6, 10 mM MgCl 2 , 10 mM D...ATP, and 25 g/ml BSA), and 0.1 Weiss unit/l T4 DNA ligase (Bio-Rad). All ligations reactions were incubated at 16 C overnight. The ligase was heattreated by placing the mixtures...

Get the 5'-End

... Liu, X. and Gorovsky, M.A.1993. Mapping the 5' and 3' ends of Tetrahymena thermophila mRNAs using RNA ligase mediated amplification of cDNA ends (RLM-RACE). Nucl. Acid Res. 21 :4954-4960 Who Needs...

Cloning Based on Efficient Three-Fragment Assembly DNA Ligation

...ses. Therefore, we started the reaction at 26C, which was reported to be the optimal temperature for the joining of bluntended termini by T4 DNA ligase [7]. We followed this with cohesive-end ligation and circularization of our DNA at 14C, the temperature generally regarded as the best compromis...

Eppendorf Perfectprep Gel Cleanup Kit

...p Alkaline Phosphatase (Promega ) T4 DNA ligase (New England BioLabs) TOP 10 Chemically C... end units (about 6 Weiss units) of T4 DNA ligase and 5% PEG 8000 were incubated at 14C overnight (15 hours). Following this, the ligase was inactivated by incubating the sampl...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

...ting insert and vector in the presence of T4 DNA ligase and Srf I enzyme reduces the background of self-...ng reactivity of the Srf I enzyme and the T4 DNA ligase on nonrecombinant vector DNA. In many instan...r polishing, 3 Srf I restriction enzyme, T4 DNA ligase and Epicurian Coli XL10-Gold ultracompetent ...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

...od can be used for high-throughput 3 EST sequencing and for functional screening. Additionally, this method does not depend on the use of T4 DNA ligase to covalently link the vector and insert; consequently, the background of self-ligated vector is reduced. Since PCR products are cloned without en...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

...cess of enzyme, and the enzyme is removed using StrataClean resin. The digestion products are ligated using Stratagenes T4 DNA ligase and then recut with the same restriction enzyme. Samples of the cut, ligated, and recut DNA are subsequently analyzed by agarose gel electr...

Enhanced PCR Cloning System

...zed vector and increased ligation efficiency for blunt-ended DNA fragments by the simultaneous, opposing reactivity of the Srf I enzyme and T4 DNA ligase on nonrecombinant vector DNA. Ligated DNA is then ready to be transformed into competent cells. The PCR-Script kits, although ideal to use with ...
Ligase in Biological Products

Ready-To-Go T4 DNA Ligase from GE Healthcare, formerly Amersham Biosciences

Description: ...
Company:GE Healthcare, formerly Amersham Biosciences

T4 DNA Ligase from Invitrogen

Description: T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates f...
Company:Invitrogen

T4 DNA Ligase from Promega

Description: T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration (1). The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in...
Company:Promega

T4 RNA Ligase from Roche Applied Science

Description: Request Info...
Company:Roche Applied Science

T4 DNA Ligase (HC) from Invitrogen

Description: Request Info...
Company:Invitrogen

Zero Blunt TOPO PCR Cloning Kit with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli from Invitrogen

Description:...rmostable polymerases. The kit includes the linearized and topoisomerase I-activated pCR-Blunt II-TOPO vector for 5-minute bench-top ligations without ligase (1). The pCR-Blunt II-TOPO vector also features:...
Company:Invitrogen

EcoR I from USB Corp.

Description:...un/l High Conc.[PN 70658]: 30 - 60 un/l Ligation-Redigestion: After 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA ligase and re-digested with this enzyme. Includes (1 ml each): 10X High Buffer and 10X Loading Buffer. A USB Floatboat will be sent free with every USB Restr...
Company:USB Corp.

Bgl I from USB Corp.

Description:...: Standard Conc.[PN 70543]: 8 - 20 un/l Ligation-Redigestion: After 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA ligase and re-digested with this enzyme. Includes (1 ml each): 10X Bgl I Buffer and 10X Loading Buffer. A USB Floatboat will be sent free with every USB Rest...
Company:USB Corp.

Ligase, T4 DNA from USB Corp.

Description:...ducing clone of T4 DNA Ligase. Description: T4 DNA ligase catalyzes the formation of a phosphodiester bond b...n dry ice. Store at -20C. Assay Conditions: T4 DNA ligase is assayed according to the pyrophosphate exchange... for the reaction. This contrasts with E. coli DNA ligase which requires NAD. Unit Definition: One Weiss uni...
Company:USB Corp.

Rabbit Anti-Human COP9 signalosome complex subunit 2 Polyclonal Antibody, Unconjugated from Aviva Systems Biology

Description:... CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP. COPS2 is also innvolved ...
Company:Aviva Systems Biology

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Ligase in Biological Definition

Sticky end/blunt end

...TpGpApCpT pGpApTpGpCpGpTpApTpGpCpT -3' 3'- TpApGpApCpTpGpApCpTpApCpGp CpApTpApCpGpA -5' The two fragments may then be covalently bonded by DNA ligase . Blunt ends may be ligated, but the reaction is significantly slower. These terms are most commonly used to describe the product of a restriction ...

Lagging strand

...the fragments, removes the RNA, and adds its own nucleotides (this is necessary because RNA and DNA use slightly different kinds of nucleotides). DNA ligase joins the fragments together. ...

Okazaki fragment

...otide is removed by and synthesized by Pol I . The nick, or a broken phosphodiester bond remaining between the fragments is linked together by DNA ligase . ...
Ligase in Biological Dictionary

Ligase

In biochemistry, a ligase is an enzyme that can catalyse the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small chemical group pendant to one of the larger molecules. Generally ligase catalyses the following... In molecular biol...

DNA ligase

In molecular biology, DNA ligase is a special type of ligase (EC 6.5.1.1) that can link together two DNA strands that have double-strand break . The alternative, a single-strand break, is fixed by a different type of DNA ... DNA ligase This article does not cite any references or so...

Ubiquitin

...m and nucleus of all eukaryotes Ubiquitin ligase A ubiquitin ligase (also called an E3 ubiquitin ligase) is a prot...ss ... negative regulation of ubiquitin -protein ligase activity ... The diverse roles of ubiquitin ...

S phase

...c cycle during which DNA ... S phase . ( 2009) ... Cell cycle articles relating to ' S phase inhibitors' ... Role of the SCFSkp2 ubiquitin ligase in the degradation of p21Cip1 in S phase . ... ... activity is restricted to the G1- S phase of the cell cycle. ... During S phase , cy...

Saccharomyces cerevisiae

...such as Saccharomyces cerevisiae are single-celled fungi which that ... Saccharomyces cerevisiae cdc9, a structural gene for yeast DNA ligase which ... Endonuclease alpha from Saccharomyces cerevisiae shows increased activity on ... synteny blocks of current Saccharomyces cerevisi...

Ribozyme

... the hydrolysis of bonds in other RNAs, but they have also... 6 The L1 ligase Ribozyme Crystal Structure. 6.1 A Three-Dimensional Glimpse of The First Enzyme ... More details on the ligase Ribozyme . ... ... correspond to substrate and ribozyme as in ref...

Recombinant DNA molecule

...r Research Involving Recombinant DNA Molecules ... ... the NIH guidelines, recombinant DNA are molecules constructed outside ... DNA ligase is used to covalently link the two strands into a molecule of recombinant ... ... Involving Recombinant DNA Molecules ) for information n...

Primer extension

.... Polymerase-driven primer extension across this span of DNA sequence is ... 2.4 Primer extension . 2.5 5'- nuclease. 2.6 Oligonucleotide ligase assay ... Because, primer extension is based on the highly accurate DNA polymerase enzyme, ... Predicting the success of primer extension...

Primase

...ucture of the Helicase-Interaction Domain of the Primase DnaG ... 1.3 Primase . 1.4 DNA polymerase III holoenzyme. 1.5 DNA polymerase I. 1.6 ligase ... continue, but not begin, a strand, Primase must begin the leading strand as well. ... ... has both primase and helicase ... this study,...

Okazaki fragments

...nition and other additional information on Okazaki fragments from Biology-Online.org dictionary. ... Re: Okazaki fragments . Would they be RNA ligase or DNA ... Nature is the international weekly journal of science: a magazine style journal that publishes full-length research ... short ' Okaza...
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