LacZ

Weill Cornell team identifies new cancer stem cell driving metastatic tumors

...ion the researchers generated a transgenic mouse in which the CD133 gene is replaced with a reporter gene called lacZ. "We relied on the expression of lacz to detect the spatial and temporal location of CD133+ cells in vivo," explains co-researcher Andrea T. Hooper, a graduate student in Dr. Rafii's lab. ...

LacZ in Biological News

Molecule by molecule, new assay shows real-time gene activity

...fuse fluorescent signals tend to be overwhelmed by background noise. The gene coding for this combined protein was substituted for the well-studied lacz gene in the Escherichia coli chromosome. When lacZ's regulatory machinery allows the modified gene to be converted into a handful of protein molecules...

LacZ in Biological Technology

Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses

...c Acids Res., 20 , 245-250 (1992). 4. Hall, C. V., Jacob, P. E., Ringold, G. M. and Lee, F. Expression and regulation of E. coli lacz gene fusions in mammalian cells. J. Mol. Appli. Genet., 2 , 101-109 (1983). 5. DeWet, J. R., Wood, K. V., DeLuca, M., Helinski, ...

Transformation of Nematodes With the Helios Gene Gun

...), or of the C. elegans actin-1 core promoter after Helios gene gun mediated delivery of Au/DNA particles into the nematode. The lacz expression of constructs containing the actin-1 core promoter of C. elegans (Files et al., 1983; Krause et al., 1989; Stone and Shaw, 1993)...

Electrotransformation of E. coli With M13 DNA

... DNA is a superior starting material for sequencing and in vitro mutagenesis. In addition, the blue/white selection process afforded by the lacz fusion cloning scheme allows easy detection of recombinant phage over background. During the process of evaluating and optimizin...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

...tive technique to measure the concentration of pHSVlac for each plasmid preparation. We used a primer/probe set specific for the lacz gene and an ABI PRISM 7700 sequence detector (PE Corporation). Reaction mixtures (50 l) contained 1 ng of DNA (by absorbance),...

Eppendorf Perfectprep Gel Cleanup Kit

...om Integrated DNA Technologies (Coralville, IA, USA): lacz F1 (5'-GGAAAGCTGGCTGGAGTGCGATCTT-3') lacz R2 (5'-TCCCCAGCGACCAGATGATCACACT-3'). A final concentration of 4...

Eppendorf Perfectprep Gel Cleanup Kit

...ning capability of gel-purified DNA fragments. pUC19 contains the lacz gene allowing for blue/white colony screening as an initial measure of cloning success. White colonies indicate that the lacz gene was disrupted and the cloning was successful. Based on the ...

hNT BlueNeurons Express b-Galactosidase at High Levels

...ession vector used for transfecting NT2 precursor cells with the lacz gene had to fulfill several crucial criteria: integration, not i...mycinresistance gene controlled by the SV40 early promoter and a lacz gene controlled by the Rous sarcoma virus (RSV) promoter. Ge...

Randomize Gene Sequences with New PCR Mutagenesis Kit

...eneMorph kit was demonstrated in studies employing lacz as the mutational target gene. A 650-bp...NA fragment containing a portion of the lacz gene was amplified with Mutazyme DNA polymerase fr... The frequency of mutations in the lacz gene was determined for numerous PCR reactions emp...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

...escript II SK phagemid vector ( Figure 3 ), which contains the lacz reporter gene, is cut at a single position within the multiple c...ion reaction is compromised (i.e., a nucleotide is deleted), the lacz gene is interrupted, and white colonies result. Enzymes tested i...

Highest Possible Transformation Efficiencies for High-Throughput Applications

...DNA, we transformed 96Pack Gold cells and MultiShot cells with 2.3 ng of a ligated construct, the pCMV-Script plasmid with the lacz gene insert (approximately 4.9 kb). For each experiment, we followed the manufacturers protocols. As can be seen, 96Pack Gold co...

LacZ in Biological Products

pORF-LacZ from InvivoGen

Description:... Plasmid expressing the lacz reporter gene....
Company:InvivoGen

LacZ in Biological Dictionary

Transversion

...Y-2 gene only a fivefold increase was ... Conversely, GC TA transversion increased in mutL or mutS mutants in stationary phase. ... The rate of lacz GC TA transversion was determined by the method of Lea and ... 2. Which of the substitutions in question 1 are transversion mutations? ANSWER: ...

Reporter Gene

... ... GFP expression from reconstituted reporter genes . 3. References ... ... be cited as: Boulin, T. et al. Reporter gene ... of GFP over lacz is the ability to visualize reporter gene expression in ... reporter gene ... Encyclopedia information on Reporter Gene ... A commonl...