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LIC in Biological Technology

New Competent Cells for Highest Transformation Efficiencies

...fficiency of transforming XL10-Gold cells with a ligated DNA molecule. The pCAL-n-EK vector (8.0 kb) from Stratagenes Affinity lic cloning kit was digested and prepared for the ligation-independent cloning (LIC) reaction using standard conditions. 2 The kana...

Randomize Gene Sequences with New PCR Mutagenesis Kit

... PCR primers were designed to introduce lic (ligation independent cloning) sites for cloning into the Affinity lic vector. Mutazyme produced high yields of ...struct. The PCR primers were designed to introduce lic (ligation independent cloning) sequence...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

... cDNA synthesized from small amounts of RNA with lic for high-efficiency, directional cloning. Th... by PCR using primers with specifically designed lic ends. 1-3 The PCR products are ready for purifi...irectional cloning into a vector with compatible lic overhangs. The kit offers numerous advantages over...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

...ired protein sequence into the pCAL-n-EK vector. lic creates seamless cloning junctions between the p...equire ligation or restriction enzyme digestion. lic creates a seamless junction 4,5,6 between the EK ... flexibility, Stratagene has refined the lic method for cloning inserts into the pCA...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

...tagene Stratagene has improved the Affinity lic cloning kit with the new pCAL-n-FLAG vector. Thi... protein coding sequence of interest, cloning by lic is highly efficient. Cloning efficiencies of 2... (MCS), and the T7 transcriptional terminator. lic Cloning of JNK ...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

...er Stratagene Cloning Systems, Inc. The ESP lic cloning and expression system features rapid and h... figure 1 Stratagenes ESP lic cloning and expression system offers rapid ...tor offers is efficient and directional lic 2-4 cloning of PCR products immediately ...

A New C-Terminal GST Vector for Protein Production in S. pombe

...roducts through ligation-independent cloning (LIC). 12 When the lic method is used, polypeptides without extraneously added amino ac...available separately and as components of the ESP system and ESP lic systems, respectively. The ESP system has been extensively teste...

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LIC in Biological Products

NovaTaq DNA Polymerase from Novagen

Description:... exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagens Perfectly Blunt, AccepTor, and lic Vector Kits. Each order also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10 No...
Company:Novagen

NovaTaq DNA Polymerase from Novagen

Description:... exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagens Perfectly Blunt, AccepTor, and lic Vector Kits. Each order also includes optimized 10X NovaTaq Buffer with 15 mM MgCl 2 for routine amplification conditions, plus separate vials of 10 ...
Company:Novagen

LIC Duet™ Trx•Tag™ Ek Adaptor from Novagen

Description:... The lic Duet™ Trx•Tag™ Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an...
Company:Novagen

LIC Duet™ GST•Tag™ Ek Adaptor from Novagen

Description:... The lic Duet™ GST•Tag™ Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon and an ...
Company:Novagen

LIC Duet™ T7•Tag® Ek Adaptor from Novagen

Description:... The lic Duet™ T7•Tag ® Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an ...
Company:Novagen

LIC Duet™ Minimal Adaptor from Novagen

Description:... The lic Duet™ Mini Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), and an ATG translation initation codon. This adaptor is d...
Company:Novagen

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(Date:4/17/2014)... April 17, 2014 Meaningful long-term survival ... cancer of the abdomen when treated with cytoreductive ... to a first-of-its-size analysis by physicians at Wake ... has the largest reported, single-center experience with cytoreductive ... Levine, M.D., and analysis of 20 years, worth ...
(Date:4/17/2014)... cent of Canadians surveyed said they would participate in ... number of genetic conditions, only 80 per cent said ... would sequence their newborns, genomes.,Most newborns in North America ... or two of life in which a tiny amount ... for about five to 54 conditions, depending on the ...
(Date:4/17/2014)... more research to be carried out into HIV ... In a paper in The Lancet Infectious ... colleagues in the department of Parasitology and researchers ... University of Cambridge and the Royal Veterinary College ... of HIV/AIDS and schistosomiasis of children, and found ...
(Date:4/16/2014)... SAN ANTONIO, Texas (April 16, 2014) A team that ... of Texas Health Science Center at San Antonio, Johns Hopkins ... protein that helps a common fungus to infect the body. ... infections, diaper rashes and oral thrush, and is the most ...
(Date:4/15/2014)... Handling frozen fish caused nearly half of all injuries ... injuries on freezer-longliner vessels operating off the coast of ... Many of those injuries and others aboard the two ... interventions, and the research methods used in the study ...
Breaking Medicine News(10 mins):Health News:20 years of data shows treatment technique improvement for advanced abdominal cancer 2Health News:Researcher looks at public perceptions around newborn testing 2Health News:Researcher looks at public perceptions around newborn testing 3Health News:More research called for into HIV and schistosomiasis coinfection in African children 2Health News:Changes in processing, handling could reduce commercial fishing injuries 2
(Date:4/17/2014)... new early-warning signs of the potential loss of sight ... for the diagnosis and treatment of diabetic retinopathy, potentially ... "We had not expected to see such striking changes ... Elsner, professor and associate dean in the IU School ... set out to study the early signs, in volunteer ...
(Date:4/17/2014)... University of Texas Medical Branch at Galveston are ... with radiation for cervical cancer should begin colorectal ... The UTMB researchers, finding a high incidence of ... with radiation, offer new recommendations that the younger ... about eight years after their initial cervical cancer ...
(Date:4/17/2014)... way the immune system may fight cancers and viral ... cells to treat illness. , The research, in mice, ... "neighborhood police" specialized squads of defenders that patrol ... entire city, the body. , Scientists at Washington University ... the liver, skin and uterus each has dedicated immune ...
Breaking Biology News(10 mins):New technique detects microscopic diabetes-related eye damage 2New technique detects microscopic diabetes-related eye damage 3Radiation therapy for cervical cancer increases risk for colorectal cancer 2Some immune cells defend only 1 organ 2
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