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LIC in Biological Technology

New Competent Cells for Highest Transformation Efficiencies

...fficiency of transforming XL10-Gold cells with a ligated DNA molecule. The pCAL-n-EK vector (8.0 kb) from Stratagenes Affinity lic cloning kit was digested and prepared for the ligation-independent cloning (LIC) reaction using standard conditions. 2 The kana...

Randomize Gene Sequences with New PCR Mutagenesis Kit

... PCR primers were designed to introduce lic (ligation independent cloning) sites for cloning into the Affinity lic vector. Mutazyme produced high yields of ...struct. The PCR primers were designed to introduce lic (ligation independent cloning) sequence...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

... cDNA synthesized from small amounts of RNA with lic for high-efficiency, directional cloning. Th... by PCR using primers with specifically designed lic ends. 1-3 The PCR products are ready for purifi...irectional cloning into a vector with compatible lic overhangs. The kit offers numerous advantages over...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

...ired protein sequence into the pCAL-n-EK vector. lic creates seamless cloning junctions between the p...equire ligation or restriction enzyme digestion. lic creates a seamless junction 4,5,6 between the EK ... flexibility, Stratagene has refined the lic method for cloning inserts into the pCA...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

...tagene Stratagene has improved the Affinity lic cloning kit with the new pCAL-n-FLAG vector. Thi... protein coding sequence of interest, cloning by lic is highly efficient. Cloning efficiencies of 2... (MCS), and the T7 transcriptional terminator. lic Cloning of JNK ...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

...er Stratagene Cloning Systems, Inc. The ESP lic cloning and expression system features rapid and h... figure 1 Stratagenes ESP lic cloning and expression system offers rapid ...tor offers is efficient and directional lic 2-4 cloning of PCR products immediately ...

A New C-Terminal GST Vector for Protein Production in S. pombe

...roducts through ligation-independent cloning (LIC). 12 When the lic method is used, polypeptides without extraneously added amino ac...available separately and as components of the ESP system and ESP lic systems, respectively. The ESP system has been extensively teste...

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LIC in Biological Products

NovaTaq DNA Polymerase from Novagen

Description:... exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagens Perfectly Blunt, AccepTor, and lic Vector Kits. Each order also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10 No...
Company:Novagen

NovaTaq DNA Polymerase from Novagen

Description:... exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagens Perfectly Blunt, AccepTor, and lic Vector Kits. Each order also includes optimized 10X NovaTaq Buffer with 15 mM MgCl 2 for routine amplification conditions, plus separate vials of 10 ...
Company:Novagen

LIC Duet™ Trx•Tag™ Ek Adaptor from Novagen

Description:... The lic Duet™ Trx•Tag™ Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an...
Company:Novagen

LIC Duet™ GST•Tag™ Ek Adaptor from Novagen

Description:... The lic Duet™ GST•Tag™ Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon and an ...
Company:Novagen

LIC Duet™ T7•Tag® Ek Adaptor from Novagen

Description:... The lic Duet™ T7•Tag ® Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an ...
Company:Novagen

LIC Duet™ Minimal Adaptor from Novagen

Description:... The lic Duet™ Mini Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), and an ATG translation initation codon. This adaptor is d...
Company:Novagen

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