Navigation Links
LIC in Biological Technology

New Competent Cells for Highest Transformation Efficiencies

...fficiency of transforming XL10-Gold cells with a ligated DNA molecule. The pCAL-n-EK vector (8.0 kb) from Stratagenes Affinity lic cloning kit was digested and prepared for the ligation-independent cloning (LIC) reaction using standard conditions. 2 The kana...

Randomize Gene Sequences with New PCR Mutagenesis Kit

... PCR primers were designed to introduce lic (ligation independent cloning) sites for cloning into the Affinity lic vector. Mutazyme produced high yields of ...struct. The PCR primers were designed to introduce lic (ligation independent cloning) sequence...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

... cDNA synthesized from small amounts of RNA with lic for high-efficiency, directional cloning. Th... by PCR using primers with specifically designed lic ends. 1-3 The PCR products are ready for purifi...irectional cloning into a vector with compatible lic overhangs. The kit offers numerous advantages over...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

...ired protein sequence into the pCAL-n-EK vector. lic creates seamless cloning junctions between the p...equire ligation or restriction enzyme digestion. lic creates a seamless junction 4,5,6 between the EK ... flexibility, Stratagene has refined the lic method for cloning inserts into the pCA...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

...tagene Stratagene has improved the Affinity lic cloning kit with the new pCAL-n-FLAG vector. Thi... protein coding sequence of interest, cloning by lic is highly efficient. Cloning efficiencies of 2... (MCS), and the T7 transcriptional terminator. lic Cloning of JNK ...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

...er Stratagene Cloning Systems, Inc. The ESP lic cloning and expression system features rapid and h... figure 1 Stratagenes ESP lic cloning and expression system offers rapid ...tor offers is efficient and directional lic 2-4 cloning of PCR products immediately ...

A New C-Terminal GST Vector for Protein Production in S. pombe

...roducts through ligation-independent cloning (LIC). 12 When the lic method is used, polypeptides without extraneously added amino ac...available separately and as components of the ESP system and ESP lic systems, respectively. The ESP system has been extensively teste...

More>>

LIC in Biological Products

NovaTaq DNA Polymerase from Novagen

Description:... exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagens Perfectly Blunt, AccepTor, and lic Vector Kits. Each order also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10 No...
Company:Novagen

NovaTaq DNA Polymerase from Novagen

Description:... exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagens Perfectly Blunt, AccepTor, and lic Vector Kits. Each order also includes optimized 10X NovaTaq Buffer with 15 mM MgCl 2 for routine amplification conditions, plus separate vials of 10 ...
Company:Novagen

LIC Duet™ Trx•Tag™ Ek Adaptor from Novagen

Description:... The lic Duet™ Trx•Tag™ Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an...
Company:Novagen

LIC Duet™ GST•Tag™ Ek Adaptor from Novagen

Description:... The lic Duet™ GST•Tag™ Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon and an ...
Company:Novagen

LIC Duet™ T7•Tag® Ek Adaptor from Novagen

Description:... The lic Duet™ T7•Tag ® Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an ...
Company:Novagen

LIC Duet™ Minimal Adaptor from Novagen

Description:... The lic Duet™ Mini Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), and an ATG translation initation codon. This adaptor is d...
Company:Novagen

More>>

Other Tags
(Date:10/18/2014)... The famous review site Top10BestSEOHosting.com has recently compared many ... suppliers carefully, today it announces that HostMonster, GreenGeeks and ... first half of 2014. , HostMonster is an ... package helps business companies and individuals get high-powered service ... rated hosting provider, it insists on offering outstanding customer ...
(Date:10/18/2014)... (PRWEB) October 19, 2014 ... announced that Hostgator ( http://secure.hostgator.com/~affiliat/cgi-bin/affiliates/clickthru.cgi?id=seohosts ), JustHost ... suppliers in 2014. , “There are ... world, but good suppliers are not too ... and FatCow are the most outstanding suppliers ...
(Date:10/18/2014)... Operators in the Carpet ... carpet cleaning services, such as steam cleaning, dry-cleaning ... every one to two years. According to IBISWorld ... plummeted during the peak of the global financial ... carpet cleaning.” The upward trend in household discretionary ...
(Date:10/18/2014)... TX (PRWEB) October 18, 2014 ... Chinese Chondroitin sulfate Industry, 2009-2019 is a ... Global and Chinese Chondroitin sulfate industry. The ... Chondroitin sulfate including its classification, application and ... and China’s top manufacturers of Chondroitin sulfate ...
(Date:10/18/2014)... determined dieters can fail if they don,t have a ... Their new study included 240 obese people. All of ... factors that increase the risk of heart disease, stroke ... to make lifestyle changes, including improved eating, the study ... clinical trial comparing two dietary interventions: the American ...
Breaking Medicine News(10 mins):Health News:Top10BestSEOHosting.com: HostMonster, GreenGeeks And FatCow Are The Best PHP Hosting Suppliers In The First Half Of 2014 2Health News:Top10BestSEOHosting.com: Best Reseller Hosting Companies Announced 2Health News:Carpet Cleaning Services in Australia Industry Market Research Report Now Available from IBISWorld 2Health News:Carpet Cleaning Services in Australia Industry Market Research Report Now Available from IBISWorld 3Health News:Chondroitin Sulfate Industry Global & Chinese Forecast to 2019 Now Available at RnRMarketResearch.com 2Health News:Chondroitin Sulfate Industry Global & Chinese Forecast to 2019 Now Available at RnRMarketResearch.com 3Health News:Chondroitin Sulfate Industry Global & Chinese Forecast to 2019 Now Available at RnRMarketResearch.com 4Health News:Dieters May Be Thwarted by Absence of Healthy Foods 2
(Date:10/17/2014)... To date, antidotes ... doctors are often limited to supportive therapy such as induced ... of drugs involved. So what can be done if a ... professor Jean-Christophe Leroux from the Institute of Pharmaceutical Sciences at ... "The task was to develop an agent that could eliminate ...
(Date:10/16/2014)... Mary,s Virginia Institute of Marine Science have received ... Agency to identify the streams and wetlands most ... to help local governments and citizens conserve these ... a dataset of tidal-marsh observations first gathered by ... historical baseline can today,s researchers accurately map the ...
(Date:10/16/2014)... the bowel lining develops and, in the process, suggested ... The researchers produced evidence that stem cells are responsible ... feature of the bowel lining, and believe these stem ... finding as scientists are still divided on the stem ... Wee Tan and Professor Tony Burgess from the Walter ...
Breaking Biology News(10 mins):Emergency aid for overdoses 2Emergency aid for overdoses 3EPA grant will help localities conserve headwater wetlands 2EPA grant will help localities conserve headwater wetlands 3Cryptic clues drive new theory of bowel cancer development 2
Other Contents