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The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System

CXCR2 • GPCR • receptor binding assay • LEADseeker • SPA Imaging Beads Chemo-attractant cytokines (chemokines) are low molecular weight, pro-inflammatory cytokines with potent leukocyte activation and/or chemotactic activity (1). Chemokines are classified into four families (CXC, CC, C, and CX...

Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System

AMARA • kinase assay • LEADseeker • chemiluminescence It is predicted that at least 500 kinase and phosphatase enzymes exist, their activities having wide-ranging regulatory responses. Many kinases are part of a signaling cascade in disease pathways such as inflammation and cancer, so identifying modulators of kinase...

The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System

Melanocortin • receptor binding assay • LEADseeker • SPA Imaging Beads Pro-melanocortins are present in high levels in the pituitary and are processed into three major peptide hormone fragments: adrenocorticotrophin (ACTH), the melanocyte stimulating hormones, and β-endorphin. The various melanocyte stimulat...

The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System

Melanocortin • receptor binding assay • LEADseeker • SPA Imaging Beads Pro-melanocortins are present in high levels in the pituitary and are processed into three major peptide hormone fragments: adrenocorticotrophin (ACTH), the melanocyte stimulating hormones, and β-endorphin. The various melanocyte stimulat...

The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System

CGRP • receptor binding assay •LEADseeker • SPA Imaging Beads Calcitonin-gene related peptide (CGRP) is believed to have an important physiological role in the central nervous and cardiovascular systems. Indeed, using CGRP receptors isolated from coronary and basilar arteries, studies have i...

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