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Knockdown in Medical News

Exiqon Announces 2009 North American Grant Award Winners

...for microRNA investigation, including products for RNA isolation, Microarray analysis, qRT-PCR analysis, Northern Blotting, In Situ Hybridization, and knockdown studies. Exiqon also offers microRNA profiling services for both microarray and qRT-PCR analysis, complete with full sample QC and customized data ana...

Peter MacCallum Cancer Centre Expands Cancer Research Efforts with Automation Tool from Caliper Life Sciences

...ng of how to treat and prevent cancer through gene knockdown therapies. "We would be unable to perform th...s with access to large scale, high throughput gene knockdown technology that aids cancer research. By eliminating a gene's ability to express, gene knockdown techniques may uncover ways to silence specific ge...

Exiqon Announces New Grant Program for MicroRNA Research

...for microRNA investigation, including products for RNA isolation, Microarray analysis, qRT-PCR analysis, Northern Blotting, In Situ Hybridization, and knockdown studies. Exiqon also offers microRNA profiling services for both microarray and qRT-PCR analysis, complete with full sample QC and customized data ana...

Castrate resistant prostate cancer: New therapeutic approaches

...l, molecular chaperone. A novel, phase I pre-prostatectomy trial defined the optimal biologically dose and toxicity parameters of OGX-011 with maximal knockdown of clusterin in prostate and lymph node tissues at the 640 mg dose level. A phase II study includes 81 men with CRPC to receive either docetaxel and O...

CSHL scientists correlate enzyme expression levels with chemotherapy drug response

...orubicin treatment, Top2A levels were dramatically reduced -- without manipulating gene expression with shRNAs. Along with the finding that Top1 knockdown hypersensitized cells for doxorubicin treatment, this result suggested to the CSHL researchers that "alterations in topoisomerase expression levels re...

Open Biosystems Launches Decode RNAi Viral Screening Pools for Low-Cost Genome-Scale RNAi Screens

...Decode RNAi viral screening set has been created using Open Biosystems' GIPZ lentiviral shRNAmir library validated in its ability to produce effective knockdown even when expressed from a single integration event in target cells. This pooled screening set targets the entire human genome in a ready-to-use lenti...

Safe, 'Green' Insecticides Can Reduce Chemical Exposure in Homes and Gardens

...l oils block specific neural pathways in insects that regulate insect movement, behavior and metabolism. This results in instant immobilization and/or knockdown followed by the insect's death. Because these neural pathways do not exist in mammals, the formulations are completely safe to use around children, an...

Researchers seek to make cavity-causing bacteria self-destruct

... answer these questions, Quiveys team has genetically engineered the first and only mutant form of S. mutans with the FabM gene removed. This FabM knockdown mutant is a living model that shows the exact impact of the enzyme in live bacteria. Without FabM, the mutant fills its outer membrane with other, sma...

Sumitomo Corporation Appoints New Chairman to U.S. Animal Healthcare Company Summit VetPharm, LLC

...onducted both in the laboratory and in the field, Vectra 3D has shown no clinically significant treatment-related adverse events, and exhibits a rapid knockdown effect. "Vectra 3D has demonstrated remarkable speed of kill and reinfestation protection in all weight ranges of dogs," according to Dr. Ahn. Vectr...

JCI table of contents: Nov. 15, 2007

... FOXP3 were found to have increased amounts of SKP2. Further, in human breast cancer cell lines overexpression of FOXP3 decreased SKP2 levels, whereas knockdown of FOXP3 expression increased SKP2 levels. Additional analysis indicated that downregulation of SKP2 expression was essential for FOXP3 to inhibit the...
Knockdown in Medical Technology

Isis Highlights New Data on Antisense Drugs to Treat Type 2 Diabetes and Obesity at ADA Scientific Sessions

... Glucose Uptake in Diet-Induced Obese Mice Authors: P. Manchem, X.X. Yu, S.L. Booten, L.M. Watts, S.F. Murray, B.P. Monia, S. Bhanot. knockdown of Carbohydrate Response Element Binding Protein (ChREBP) in Liver Protects Against Fructose Induced Hyperlipidemia and Insulin Resistance ...

Genes Identified That Enhance Tumor Cell Sensitivity to CTI's Cancer Drug Brostallicin

...lines. RNA interference is a cellular process that results in the targeted knockdown of specific genes. The current screen assayed the effect of over 7,000 ind...ere further extended and confirmed in breast cancer cell lines, wherein the knockdown of specific genes involved in these concepts, mentioned above, resulted in ...

Seahorse Bioscience Announces the XF96 Extracellular Flux Analyzer for Cellular Bioenergetics

...y expanding our ability to investigate cellular bioenergetics." "Seahorse provides a physiologically relevant assay for detecting the effect of RNAi knockdown of mitochondrial genes on cellular bioenergetics," added Vamsi Mootha, from the Broad Institute, MIT and Harvard's Massachusetts General Hospital. "Th...

Intradigm Study Shows Ability of siRNAs Systemically Delivered With TargeTran(TM) System to Suppress Tumor-Induced Angiogenesis in Mice

...trol siRNA treatment or a positive control treatment using VEGF antibody Bevacizumab. At eight days, tumor-induced angiogenesis as well as target gene knockdown were analyzed. The results showed highly significant anti-angiogenic effect that is RNAi-mediated in all active siRNA treatment groups targeting diffe...
Knockdown in Biological News

Prostate Cancer Translational Research in Europe meeting: Search for biomarkers continues

... exposure; disulfiram was not able to block tumour growth indicating the need for combined approaches. Disulfiram induced metallothionein expression, knockdown of MTI increased efficiency by five-fold. ...

Nanotechnology holds promise for STD drug delivery

...e mucosa and distributing throughout the vaginal, cervical, and uterine regions. Furthermore, the siRNAs stayed in the tissues for at least a week and knockdown of gene activity lasted up to 14 days. While past work has focused on delivery of siRNAs with liposomes, bubble-like carriers made of phospholipid...

Imiquimod, an immune response modifier, is dependent on the OGF-OGFr signaling pathway

... modulating cyclin dependent kinase inhibitors, resulting in a retardation of cells at the G1-S interface of the cell cycle. Neutralization of OGF or knockdown of OGFr by siRNA technology eliminated the inhibitory effects of imidazoquinolines on cell replication. "Thus our data," Dr. Zagon said, "brings a pa...

Centromeres cross over, a lot

...meric recombination is inhibited by protein complexes called shelterins and by DNA methylation. The centromere has no shelterin, but it is methylated. knockdown of DNA methyltransferases increased recombination at the centromere by about 50%, and decreased centromere length, possibly because of misalignment be...

Skin care: new research into scar-free healing

...l be published by the Journal of Experimental Medicine on 26 January in a paper entitled Molecular mechanisms linking wound inflammation and fibrosis: knockdown of osteopontin leads to rapid repair and reduced scarring. The paper is available online now. Speaking of the discovery, Professor Martin said: Wh...

Researchers discover important tool in understanding differentiation in human embryonic stem cells

...lopmental biologists at the University of Michigan. The researchers used knockdown technology to reduce the expression of oct4, a gene known to be necessary f...se and human embryonic stem cells. As seen in work done with mouse cells by knockdown and other genetic means, they showed that reducing the amount of oct4 in hu...

Study shows how the zebrafish gets his stripe

.... Even in the choker mutant, this bead attracted the melanophores to where it was implanted. The researchers also used a technique known as protein knockdown to reduce the amount of Sdf produced in the embryo, and were able to partially remove the third stripe in wild types and to reduce colouring in the ne...

Intrinsic eye protein halts angiogenesis

... "The standard paradigm has been the cornea is avascular because it has lots of anti-angiogenic molecules and it does," Balamurali Ambati said. "But knockdown of any of the other molecules does not cause blood vessels to enter the cornea. Knocking down SFLT-1 allows angiogenesis to take hold." The next s...

Gold nanoparticles could improve antisense cancer drugs

...mpared to antisense DNA complexed with commercial agents such as Lipofectamine and Cytofectin, the antisense nanoparticles were more effective in gene knockdown (decreasing gene expression and protein production), were less susceptible to degradation resulting in longer lifetimes, exhibited lower toxicity and ...

Attacking cancer's sweet tooth is effective strategy against tumors

...rough the knock down of LDHA), they could lower the mitochondrial membrane potential of tumor cells. What is more, oxygen consumption increased in the knockdown cells, suggesting they were reverting to the mitochondrial OXPHOS pathway--a kind of Warburg effect in reverse. "The findings provide us with an insig...
Knockdown in Biological Technology

Measuring siRNA-mediated knockdown of IL-8 mRNA

By Genospectra, Inc., 6519 Dumbarton Circle, Fremont, CA 94555. A critical step in siRNA-mediated gene expression knockdown studies is the validation of expression inhibition. The QuantiGene Reagent System and the Molecular Devices LMax II 384 microplate luminomete...

Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments

RNA interference is a powerful technique for gene knockdown experiments in academic research and an important tool for target identification and target validation in therapeutic research. ...

Quark Pharmaceuticals Presented At ARVO Data Showing That PF-04523655 Enters Retinal Cells And Elicits Its Pharmacologic Effect Via Target Gene Knock-Down Without Activating TLR3

...entific Officer, presented a study titled, "PF-04523655 (REDD14NP), an siRNA Compound Targeting RTP801, Penetrates Retinal Cells Producing Target Gene knockdown and Avoiding TLR3 Activation," at the Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting, taking place from May 3-7, 2009, in ...

Sigma-Aldrich Introduces MISSION(R) esiRNA for Low Cost, High Specificity Pooled RNAi Screening

...iminate the trial and error approach of identifying a single siRNA for gene knockdown and ensures minimal off-target effects. "MISSION esiRNA provides a pow...ng our assay for finding HIV dependency factors demonstrated very effective knockdown over a wide range of genes," said Dr. Abraham Brass, a clinical research fe...

Sigma-Aldrich Welcomes Memorial Sloan-Kettering Cancer Center to the RNAi Partnership Program

... Aldrich," said Dr. Hakim Djaballah, Director of the High Throughput Screening Core Facility. "Many of our researchers are interested in targeted gene knockdown on an individual or gene-focused basis, as well as a genome-wide scale for which we are fully equipped. Access to Sigma-Aldrich's MISSION shRNA librar...

Sigma-Aldrich Joins Phase 2 of The RNAi Consortium to Validate shRNA Libraries

...vard. A key goal of the Consortium is to provide at least two clones with a knockdown level of 70% or greater for every human and mouse gene over the next four y...with the possible creation of sub-libraries. Functional validation of these knockdown clones will provide significant time and cost savings to researchers by red...

Open Biosystems' Open Access RNAi Program Adopted by Harvard Medical School and University of North Carolina

...researchers at the University of North Carolina with the ultimate promise of RNAi by offering unrestricted access to genome scale RNAi resources. From knockdown of single genes to pathway and whole genome RNAi screening with lentiviral shRNA, this program will definitely accelerate the pace of discovery at UNC...

A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity

...ages of shRNA-based experiments include long-term knockdown and viral delivery to non-transfectable cell type...sing these cell lines. pLKO.1 allows for long-term knockdown and phenotypic observation and transduction of di... Figure 2. Screening Tumor Suppressor knockdown Cells for Paclitaxel Sensitivity. The MISSION...

FlexiPlate siRNA

...ate and save plates Cutting-edge siRNA design HP OnGuard siRNA Design minimizes the risk of off-target effects and maximizes knockdown potency Rapid delivery enabling screening without delay Maximum flexibility and economical options ...

siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

...

As would be expected following knockdown of a range of cell cycle control genes, analysis o...le distribution around the G1-S boundary following knockdown of cyclin E were not resolvable in G2/M CCPM cells...conditions (Fig 7) allowed the effects of cyclin E knockdown on G1 to S phase transition to be quantitated. Con...

Knockdown in Biological Products

21-mer duplex from B-Bridge International

Description:... siSolutions siRNA Custom Synthesis Service provides you with high-quality, effective duplex RNA to knockdown any gene. We offer standard 21-mers (19 RNA base pairs with terminal dT nucleotides), the recently-published 27-mers, or other lengths of your choice....
Company:B-Bridge International

27-mer duplex from B-Bridge International

Description:... siSolutions siRNA Custom Synthesis Service provides you with high-quality, effective duplex RNA to knockdown any gene. We offer standard 21-mers (19 RNA base pairs with terminal dT nucleotides), the recently-published 27-mers, or other lengths of your choice....
Company:B-Bridge International

21-mer duplex design + syn.; 1 gene from B-Bridge International

Description:... siSolutions siRNA Custom Synthesis Service provides you with high-quality, effective duplex RNA to knockdown any gene. We offer standard 21-mers (19 RNA base pairs with terminal dT nucleotides), the recently-published 27-mers, or other lengths of your choice....
Company:B-Bridge International

3x 21-mer duplex design + syn.; 1 gene from B-Bridge International

Description:... siSolutions siRNA Custom Synthesis Service provides you with high-quality, effective duplex RNA to knockdown any gene. We offer standard 21-mers (19 RNA base pairs with terminal dT nucleotides), the recently-published 27-mers, or other lengths of your choice....
Company:B-Bridge International

Human siTrio from B-Bridge International

Description:...tail of 3 siRNAs specifically targeted to your target gene of interestis our most popular product. The siTrio reagent guarantees you get more than 75% knockdown of your target gene when used under standard conditions (100 nM siRNA for transfection and confirmation of optimal transfection with one of B-Bridges ...
Company:B-Bridge International

Rat siTrio from B-Bridge International

Description:...tail of 3 siRNAs specifically targeted to your target gene of interestis our most popular product. The siTrio reagent guarantees you get more than 75% knockdown of your target gene when used under standard conditions (100 nM siRNA for transfection and confirmation of optimal transfection with one of B-Bridges ...
Company:B-Bridge International

Mouse siTrio from B-Bridge International

Description:...tail of 3 siRNAs specifically targeted to your target gene of interestis our most popular product. The siTrio reagent guarantees you get more than 75% knockdown of your target gene when used under standard conditions (100 nM siRNA for transfection and confirmation of optimal transfection with one of B-Bridges ...
Company:B-Bridge International

siSolutions RNAi Services from B-Bridge International

Description:... combines this service with 21-mer siRNA synthesis. siSolutions siRNA Custom Synthesis Service provides you with high-quality, effective duplex RNA to knockdown any gene. They offer standard 21-mers (19 RNA base pairs with terminal dT nucleotides), the recently-published 27-mers, or other lengths of your choic...
Company:B-Bridge International

Rat siTrio Add-on from B-Bridge International

Description:...tail of 3 siRNAs specifically targeted to your target gene of interestis our most popular product. The siTrio reagent guarantees you get more than 75% knockdown of your target gene when used under standard conditions (100 nM siRNA for transfection and confirmation of optimal transfection with one of B-Bridges ...
Company:B-Bridge International

Rat siTrio from B-Bridge International

Description:...tail of 3 siRNAs specifically targeted to your target gene of interestis our most popular product. The siTrio reagent guarantees you get more than 75% knockdown of your target gene when used under standard conditions (100 nM siRNA for transfection and confirmation of optimal transfection with one of B-Bridges ...
Company:B-Bridge International
Knockdown in Biological Definition

Reverse genetics

...d RNA, also known as RNA interference (RNAi), and the development of gene knockdown using Morpholino oligos have made disrupting gene expression an accessabl...y (e.g. the dicer proteins, the RISC complex) a simple alternative for gene knockdown is Morpholino antisense oligos. While RNAi acts by directing cellular ...
Knockdown in Biological Dictionary

Hela cell

...of apoptosis in human corneal and HeLa cells by mutated BIGH3. ... Stable Cell Lines. NFkB Reporter A549 HeLa , 293, NIH3T3 ... NFkb p50 knockdown HeLa Stable Cell Line. AP-1 Reporter 293. GR Reporter 293. HIF Reporter NIH3T3 ... ...
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