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Kilobases in Biological News

Gene switch sites found mainly on 'shores,' not just 'islands' of the human genome

... the researchers discovered that about 76 percent of the genome's methylated sites occur a short distance away from the islands, between 200 and 2,000 kilobases away. In contrast, only 6 percent of methylated sites were situated inside CpG islands. Because of the newly discovered sites' proximity to the island...

Photosynthesizing bacteria with a day-night cycle contain rare chromosome

...tein that lets the bugs produce lactate, called lactate dehydrogenase, during fermentation. DNA sequencing revealed the linear chromosome to be 430 kilobases long and contain a cluster of nine genes that code for other enzymes involved in pyruvate metabolism. These allow Cyanothece 51142 to make ethanol, ...

Cyanobacterium sequenced features rare linear chromosome

...nd the presence of a rare linear chromosome in the organism's genome, a first in cyanobacteria. Further examination revealed the chromosome to be 430 kilobases long and containing a cluster of nine genes that code for enzymes involved in pyruvate metabolism, which is the basis that allows Cyanothece 51142 t...

'Early bird' project really gets the worm

... scope and in terms of the amount and type of data we collected," said Marks. Thus far, scientists have built and analyzed a dataset of more than 32 kilobases of nuclear DNA sequences from 19 different locations on the DNA of each of 169 bird species. "This paper makes tremendous strides toward determinin...

Exploring the dark matter of the genome

... flies and produce libraries of DNA fragments of two sizes, some that are 2 kilobases long" -- a kilobase (Kb) is 1,000 bases -- "and some that are 10 kilobases long. Contiguous lengths of sequence are assembled by matching overlaps of ...

Major obesity gene is 'lost in the shuffle'

...IP gene. Our study shows that during the evolution of gibbons, these Alu elements lined up and recombined with each other, erasing approximately 100 kilobases of DNA that spanned the ASIP gene." Ranging in size from 15 to 22 pounds, gibbons possess lithe, slender bodies that enable them to swing agilely...

Where 'jumping genes' fear to tread

...and, Australia, identified long tracks of genomic segments (greater than 10 kilobases in length) that lack transposable elements. His team identified 860 such s...er, it's difficult to explain mechanistically the requirement of 10 or more kilobases of uninterrupted sequence in terms of the current paradigms of transcriptio...

Reversal of role for a viral protein associated with the development of lymphoma

...NA," Lopez said. Lopez found that predicted recursive splice sites were 10 times more likely than expected to be found in introns greater than 200 kilobases in length, and 92 percent of them were conserved over at least 25 million years of insect evolution. This discovery strongly suggests that recursive s...

Carnegie Mellon University research reveals how cells process large genes

...NA," Lopez said. Lopez found that predicted recursive splice sites were 10 times more likely than expected to be found in introns greater than 200 kilobases in length, and 92 percent of them were conserved over at least 25 million years of insect evolution. This discovery strongly suggests that recursive s...
Kilobases in Biological Technology

Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping

Contributed by Donald R. VanDevanter, Tumor Institute, Swedish Hospital Medical Center, 409 Elkind Hall, 747 Summit Avenue, Seattle, WA 98104 Introduction Analyses of uncharacterized DNA fragments using CHEF electrophoresis often req...

SNPs Powerful Tools for Association Studies

...he first genetic markers to be widely used in genetic mapping studies. To identify RFLPs, genomic DNA is digested into small pieces, averaging several kilobases (kb), by restriction enzymes that recognize specific sequences (i.e., restriction sites) in the genome. When genomic variation between individuals eli...

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

...Yale University, 333 Cedar Street, New Haven, CT 06510. Introduction Genes of higher eukaryotes can be hundreds of kilobases in size. The need to rapidly clone and manipulate DNA molecules in that range has become acute in conjunction with the Human Gen...

DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell

... DNA Transfers in 10 Minutes PCR* fragments, plasmid, and vector DNA ranging in size from several hundred bases to 15 kilobases can now be quickly transferred in as little as 10 minutes. Set-up time is reduced to just minutes because hydrolyzation of DNA f...

Got Small RNA?

...mized to prevent loss of the small RNA fraction. The fast and efficient GFF-based method isolates total RNA ranging in size from kilobases down to 10-mers. Figure 1 demonstrates that RNA isolated by the
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(Date:9/22/2014)... ON (September 22, 2014) McMaster scientists have found ... new class of antibiotics. , Although dozens of ... at how a certain part of bacteria are created, ... it. , The discovery is important as there ... making the cures for infections ineffective. The World Health ...
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(Date:9/22/2014)... 2014 NXT-ID, Inc. (NASDAQ: NXTD ... mobile commerce market, issues the following corporate shareholder update ... Letter from the CEO : ... funding and joined the NASDAQ to support ramping of ... biometric and authentication products and services. This strategic achievement positions ...
(Date:9/21/2014)... significantly less likely to report taking iron supplements before ... who are developing normally, a study by researchers with ... iron intake was associated with a five-fold greater risk ... 35 or older at the time of the child,s ... as obesity hypertension or diabetes. , The research is ...
(Date:9/21/2014)... at the University of California, San Diego, have developed ... that control the activity of genes based on sequences ... from its application to human embryonic cells in a ... 21. , "All of our cells have the same ... said John Whitaker, lead author of the report. "Skin ...
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