Exploring the dark matter of the genome
... shotgun sequencing. Celniker says, "We grind up whole flies and produce libraries of DNA fragments of two sizes, some that are 2 kilobases long" -- a kilobase
(Kb) is 1,000 bases -- "and some that are 10 kilobases long. Contiguous lengths of sequence are assembled by matching overlaps of these fragments. Wit...
Kilobase in Biological Technology
Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping
...one gel in
order to fully characterize large DNA fragments from a single cell source 1 .
This is because DNA fragments under 200 kilobase
pairs (kbp), between
200 kbp and 2 megabase pairs (mbp), and between 2 mbp and 6 mbp are optimally
separated by CHEF electrophor...
Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
2. Sternberg, N., Bacteriophage P1 cloning system for the isolation,
amplification, and recovery of DNA fragments as large a 100 kilobase
Proc. Natl. Acad. Sci. USA, 87 , 103107 (1990).
3. OConnor, M., Peifer, M., and Bender, W., Construction of large DNA
Northern Analysis: The Basics
...te determination of mRNA size. The
Millennium Marker mixture includes 0.5, 1, 1.5, 2, 2.5, 3, 4,
5, 6, and 9 kilobase
transcripts that can be radioactively
labeled, probed with our Millennium Marker Probe, or ethidium
Isolate It All: siRNA miRNA
Total RNA Native Protein
...rates the broad range of RNAs that can be obtained
with this procedure, from miRNAs (~22 nt) to much larger mRNA species
(in the kilobase
Versatile Isolation System
In summary, these results show
Kilobase in Biological Definition
...l around the other. The strands can also be separated by gentle heating, as used in PCR , provided they have fewer than about 10,000 base pairs (10 kilobase
pairs, or 10 kbp). The intertwining of the DNA strands makes long segments difficult to separate.
When the ends of a piece of do...
Kilobase in Biological Dictionary
Base pair. From Wikipedia, the free encyclopedia (Redirected from Kilobase
) ... A kilobase
(kb) is a unit of measurement in molecular biology equal to 1000 base ... Definition of kilobase
from the Merriam-Webster Online Dictionary with audio pronunciations, thesaurus, Word of the D...
...nsertion occurs in a part of the gene known as the 3' untranslated region, ... DNA ; extracellular ; extracellular matrix ; gene ; insertion ; kilobase
; ... Gene , insertion ,biological ,biology dictionary,biology terminology,biology terms,biology abbreviations ... Targeted Gene Insertion .....