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Kanamycin in Biological News

Herbicide-resistant grape could revitalize Midwest wine industry

...t actually contain the gene," said Skirvin. He explained that in order to locate the cells that have the gene, another gene that's an antibiotic to kanamycin is inserted as a marker. The cells are then placed onto a medium that's very high in Kanamycin. All of the normal cells die. The only ones that will ...
Kanamycin in Biological Technology

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

...tte from plasmid pRSVneo), and 3) the polylinker from the pBluescript SK cloning vector. The vector stably confers resistance to kanamycin in E. coli, and resistance to G418 in mammalian cells. The plasmid is approximately 12 kb in size, and, like its F factor parent, ...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

... lines. In addition, the neomycin-resistance gene is under control of the prokaryotic b -lactamase promoter to provide kanamycin resistance in bacteria as well as the SV40 early promoter to provide G418 resistance in mammalian cells. The pCMV-Script PCR cloning ki...

Mammalian and Bacterial Expression in One Vector

... studies using IPTG. BL21(DE3) cells harboring the KPdual-1 construct were grown in LB broth supplemented with 50 m g/ml of kanamycin until the culture reached an OD 600 of approximately 0.6. One-half of the culture was then induced by adding IPTG to a final co...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

...cludes the neomycin-phosphotransferase gene under dual control of the -lactamase and SV40 promoters, which provides selection by kanamycin resistance in bacteria and G418 resistance in mammalian cells. The multiple cloning site (MCS) of the pCMV-PCR vector is flanked...

A New Lambda Vector for Mammalian Expression

...er control of the prokaryotic b -lactamase promoter, to provide kanamycin resistance in bacteria, as well as the SV40 early promoter, to p...xcised and selected phagemid colonies by antibiotic selection on kanamycin plates. Plasmid DNA was prepared from 12 randomly picked colonie...

An Epitope Tagging Vector for Gene Expression in Mammalian Cells

... mammalian cell lines. The neomycin-resistance gene is under control of both the prokaryotic b -lactamase promoter to provide kanamycin resistance in bacteria and the SV40 early promoter to provide G418 resistance in mammalian cells. The multiple cloning site (MCS...

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

...with pAdEasy-1 viral DNA plasmid. Transformants are selected for kanamycin resistance, and recombinants are subsequently identified by rest...eplication of the viral DNA. 2 Both of these shuttle vectors contain the kanamycin resistance gene. Three test shuttle vectors, pShuttle-CMV-GFP, pShuttle...
Kanamycin in Biological Products

GIBCO Kanamycin Sulfate (100X) liquid from Invitrogen

Description: Kanamycin is used for selection of resistant bacteria. C18H36N4O11xH2SO4. Contains 10 mg/ml kanamycin (base) utilizing kanamycin sulfate in 0.85% saline....
Company:Invitrogen

GIBCO Kanamycin Sulfate from Invitrogen

Description: Request Info...
Company:Invitrogen

Origami(DE3) Glycerol Stock from Novagen

Description:... the cytoplasm. The Origami(DE3)pLacI host is compatible with expression from pETBlue and pTriEx vectors. The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, this strain is recommended for use with pET plasmids carrying the ampicillin resistance marker bla....
Company:Novagen

Origami(DE3)pLysS Glycerol Stock from Novagen

Description:... the cytoplasm. The Origami(DE3)pLacI host is compatible with expression from pETBlue and pTriEx vectors. The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, this strain is recommended for use with pET plasmids carrying the ampicillin resistance marker bla....
Company:Novagen

Zero Background/Kan Cloning Kit from Invitrogen

Description:... The Zero Background/Kan Cloning Kit includes the pZErO-2 vector with kanamycin resistance for selection in E. coli. The pZErO-2 vector utilizes positive selection to eliminate high background. The kit contains all of the reagents...
Company:Invitrogen

pPIC9K from Invitrogen

Description:... The pPIC9K and pPIC3.5K vectors carry the kanamycin resistance gene that confers resistance to Geneticin Reagent in Pichia. Spo...w in high concentrations of Geneticin indicates that multiple copies of the kanamycin resistance gene and the gene of interest are integrated into the genome (1)...
Company:Invitrogen

* Quick & Easy E. coli Gene Deletion Kit from Gene Bridges GmbH

Description:...s than one week. Red/ET recombination allows the exchange of genetic information in a base pair precise, specific, and faithful manner. An FRT-flanked kanamycin resistance marker cassette is supplied with the kit which can be used to replace a gene on the E.coli chromosome. Red/ET recombination can replace f...
Company:Gene Bridges GmbH

TargeTron Gene Knockout System from Sigma-Aldrich

Description:...ron template via PCR, ligation of the fragment into the pACD4K-C linear vector, transformation of the host, induction of gene disruption, selection by kanamycin resistance (insertion of activated marker) and screening for the desired knockout. Gene knockout using the TargeTron system has been validated in a ...
Company:Sigma-Aldrich

GPS -M Mutagenesis System from New England Biolabs

Description:...of the transposable element to your particular mutagenesis application, the transposon donor (pGPS3) can be customized by adding to or replacing the kanamycin resistance marker in the Transprimer. For ease of manipulation by the user, the donor plasmid can be grown easily in standard laboratory E. coli str...
Company:New England Biolabs

* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH

Description:.... The included FRT-PGK-gb2-neo-FRT cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of n...
Company:Gene Bridges GmbH
Kanamycin in Biological Definition

Antibiotic

...to classify antibiotics. One such classification is by chemical structure: Aminoglycosides Amikacin Dibekacin Gentamicin kanamycin Neomycin Netilmicin Paromomycin Sisomycin Streptomycin Tobramycin Beta-lactam ring antibiotics Carbapenem...
Kanamycin in Biological Dictionary

Transposon

...n many ... The existence of transposons was postulated in the late 1940s by Barbara ... [4] The transposon codes for antibiotic resistance to kanamycin and other ... FlyBase: a database for drosophila genetics and molecular biology ... The date that the natural transposon was entered into t...

Nuclear genome

...gical material. Discusses ... Cloned Rabbits Produced by Nuclear Transfer from Adult Somatic Cells - Article ... PLoS Genetics is ... genome , a kanamycin resistance gene (neo) readymade for ... integration into the nuclear genome necessitates a counterbalancing ... Nuclear Genome in Plant...

Multiple Cloning Site

...C19 contains a polylinker with unique cloning sites for multiple restriction ... the multiple cloning site , the Lac Z gene and the. kanamycin resistance gene. ... the multiple cloning site is a copy of the promoter for the lac. operon. ... ... which should recognize sites within...

Genomic clone

... small as 20kb we ... as a marker that can be used to clone and. sequence the region of genomic DNA ... Purify genomic DNA from a single. kanamycin resistant insertion clone ... Genomic cloning and identification of a novel murine Cyp4a gene. Heng, Yee M. ... The second clone , lambda...

Anaerobe

... anaerobe /an·aer·obe/ (an'ah-rōb) an organism that lives and grows in the ... BD - Diagnostic Systems: CDC Anaerobe Laked Sheep Blood Agar with kanamycin and Vancomycin (KV) ... isolation of gram-negative anaerobes was first described by ... BD - Diagnostic Systems: CDC Anaerobe 5% Sheep Blood Ag...
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