Biophysical Society announces winners of 2008 Student Travel Awards
...Dadsetan, University of California, Davis, Store-Operated Ca2+-Induced Ca2+ Release Amplifies Cytosolic Ca2+ Signaling and Prevents Store Refilling in jurkat
Angel M. Davey, The Pennsylvania State University, Dynamics Imaging of Membrane Nanostructural Changes Associated with Antigen-Mediate...
Berkeley researchers lay groundwork for cell version of DNA chip
...cules. Similar results were obtained using a line of human T-cells, called jurkat
cells, which are naturally non-adherent. Without the reactive phosphines, ...microfluidic chips through standard photolithography. After the DNA-coated jurkat
cells were rinsed over the chips, fluorescence microscopy revealed that onl...
Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
Materials and Methods
Anti-FAS monoclonal antibody
...e low-volume 384 assay format, a density of 10,000 jurkat
per well was plated with the Deer... modes, depending on the multiplex combination. jurkat
cells were treated
with various concent...
Research Tools for Analysis of ZAP-70
...ed using cell lines as well as purified B and T cells. Table 1 shows that jurkat
(human T cell line) and EL4 (mouse T cell line) cell lysates gave very good.... Oncogene 23:7990-8000.
2. Abraham, R.T. and Weiss, A. (2004) jurkat
T cells and development of the T-cell receptor signalling paradigm. Nature...
Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
protein, as a reporter gene in jurkat
cells. EGFP has an excitation maximum
set, we compared pEGFP-n2 transfected jurkat
cells with wild-type jurkat
cells. To approximate the amount of EGF...
Delivering siRNAs to Difficult Cell Types
...ermal fibroblasts-neonatal (NHDF-Neo), normal
human umbilical vein endothelial cells (HUVEC) and 2 hard-to-transfect
cell types: jurkat
and PC-12 (rat pheochromocytoma) cells. siRNA targeting
GAPDH or scrambled negative control siRNA (1.5 g) were electroporated.
Cells-to-cDNA II Using FACS Sorted Cells
...ene expression of human
-glucuronidase (hGUS) in FACS sorted jurkat
cells using real-time
RT-PCR. GUS is an enzyme constituitive...y expressed at moderate
levels in all cells.
cells were sorted by FACS and collected at
1, 10, 100 and 10...
...sential. The data shows that endotoxins reduce cell viability and transfection efficiencies among
different eukaryotic cell lines, such as COS-7 and jurkat
cells. Furthermore, certain cell lines
appear to be more sensitive to endotoxins, such as HuH-7 (human hepatoma) cells. Principally,
Detection of antibody-stained cell
surface and intracellular protein targets
with the Agilent 2100 bioanalyzer
a) Extracellular CD3 staining of Jurkat
cells, a cell line derived from
an acute human T-cell leukemia,
were harvested and washed. After
staining with calcein, the cells were
Measuring multiple apoptosis
parameters with the Agilent 2100
bioanalyzer after 6 hours treatment
with 5 M campthotecin.
After switching the instrument to
electrophoresis mode, extracted
DNA from treated jurkat
analyzed using the DNA 12000
LabChip kit. The characteristic
DNA laddering further confirmed
apoptosis in the sample (figure
Apoptosis detection by annexin V and
active caspase-3 with the Agilent 2100
Protocol for annexin V assay
1. Treat jurkat
cells at a
concentration of 5 x 105/ml
with 1 M camptothecin or
0.5 g... Protocol for caspase 3 assay
1. Treat 5 x 105/ml jurkat
with 1 M camptothecin or
0.5 g/ml anti Fas antibody for
Uses and Applications of X-tremeGENE Q2 Transfection Reagent
...gh-level protein expression in HeLa, K-562,
cells , the X-tremeGENE Q2 Transfection Reagent al...
Figure 2: jurkat
cells transfected with the X-tremeGENE Q2 Transfec...
Mouse Anti-JAB1 (Mid) Monoclonal Antibody,, Clone 3F10B10 from Invitrogen
Description:...egion of JAB1 (Jun activation domain-binding protein 1 CSN5). Specificity: Specific for the JAB1 protein. Reactivity: Human (positive controls: 293T jurkat
cell lysates). Applications: Western blotting Immunoprecipitation ELISA...
Anisomycin from Sigma-Aldrich
Description:...iting peptidyl transferase activity in eukaryote ribosomes. Reported to induce apoptosis in a variety of cells including promyelocytic leukemia cells, jurkat
cells, ventricular myocytes, and colon adenocarcinoma cells. Initiates intracellular signals and immediate early gene induction. Selective signaling a...
Rabbit Anti-SKAP55 Polyclonal Antibody, Unconjugated from ABR-Affinity BioReagents
Description:...y used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~ 60 kDa protein from human jurkat
cells.This antibody has also been used in immunoprecipitation and immunocytochemical procedures.
PA1-525 antigen corresponds to amino acids 2-...
BlockAid blocking solution *for use with microspheres* from Molecular Probes (Invitrogen)
Description:...ing of labeled microspheres. BlockAid blocking solution has been found to be effective in flow cytometry applications involving NIH 3T3, A431, RAW and jurkat
cell lines, although less effective in HMC-1 cells. We expect BlockAid blocking solution to be useful for reducing the nonspecific binding of protein-...
Company:Molecular Probes (Invitrogen)