Jurkat

Biophysical Society announces winners of 2008 Student Travel Awards

...Dadsetan, University of California, Davis, Store-Operated Ca2+-Induced Ca2+ Release Amplifies Cytosolic Ca2+ Signaling and Prevents Store Refilling in jurkat T Cells. Angel M. Davey, The Pennsylvania State University, Dynamics Imaging of Membrane Nanostructural Changes Associated with Antigen-Mediate...

Berkeley researchers lay groundwork for cell version of DNA chip

...cules. Similar results were obtained using a line of human T-cells, called jurkat cells, which are naturally non-adherent. Without the reactive phosphines, ...microfluidic chips through standard photolithography. After the DNA-coated jurkat cells were rinsed over the chips, fluorescence microscopy revealed that onl...

Jurkat in Biological Technology

Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar

... Materials and Methods jurkat cells Anti-FAS monoclonal antibody ...e low-volume 384 assay format, a density of 10,000 jurkat cells per well was plated with the Deer... modes, depending on the multiplex combination. jurkat cells were treated with various concent...

Research Tools for Analysis of ZAP-70

...ed using cell lines as well as purified B and T cells. Table 1 shows that jurkat (human T cell line) and EL4 (mouse T cell line) cell lysates gave very good.... Oncogene 23:7990-8000. 2. Abraham, R.T. and Weiss, A. (2004) jurkat T cells and development of the T-cell receptor signalling paradigm. Nature...

Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer

...ild-type protein, as a reporter gene in jurkat cells. EGFP has an excitation maximum a...r set, we compared pEGFP-n2 transfected jurkat cells with wild-type jurkat cells. To approximate the amount of EGF...

Delivering siRNAs to Difficult Cell Types

...ermal fibroblasts-neonatal (NHDF-Neo), normal human umbilical vein endothelial cells (HUVEC) and 2 hard-to-transfect cell types: jurkat and PC-12 (rat pheochromocytoma) cells. siRNA targeting GAPDH or scrambled negative control siRNA (1.5 g) were electroporated. 2...

Cells-to-cDNA II Using FACS Sorted Cells

...ene expression of human -glucuronidase (hGUS) in FACS sorted jurkat cells using real-time RT-PCR. GUS is an enzyme constituitive...y expressed at moderate levels in all cells. jurkat cells were sorted by FACS and collected at 1, 10, 100 and 10...

Introducing GenElute HP Endotoxin-Free Plasmid Maxiprep Kit

...sential. The data shows that endotoxins reduce cell viability and transfection efficiencies among different eukaryotic cell lines, such as COS-7 and jurkat cells. Furthermore, certain cell lines appear to be more sensitive to endotoxins, such as HuH-7 (human hepatoma) cells. Principally, endotoxins in...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

...eters. Results a) Extracellular CD3 staining of Jurkat cells jurkat cells, a cell line derived from an acute human T-cell leukemia, were harvested and washed. After staining with calcein, the cells were incubat...

Measuring multiple apoptosis parameters with the Agilent 2100 bioanalyzer

... bioanalyzer after 6 hours treatment with 5 M campthotecin. After switching the instrument to electrophoresis mode, extracted DNA from treated jurkat cells was analyzed using the DNA 12000 LabChip kit. The characteristic DNA laddering further confirmed apoptosis in the sample (figure 1B). ...

Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer

... Protocol for annexin V assay 1. Treat jurkat cells at a concentration of 5 x 105/ml with 1 M camptothecin or 0.5 g... Protocol for caspase 3 assay 1. Treat 5 x 105/ml jurkat cells with 1 M camptothecin or 0.5 g/ml anti Fas antibody for 224 hou...

Uses and Applications of X-tremeGENE Q2 Transfection Reagent

...gh-level protein expression in HeLa, K-562, and jurkat cells. X-tr...both reagents. In jurkat cells , the X-tremeGENE Q2 Transfection Reagent al... Figure 2: jurkat cells transfected with the X-tremeGENE Q2 Transfec...

Jurkat in Biological Products

Mouse Anti-JAB1 (Mid) Monoclonal Antibody,, Clone 3F10B10 from Invitrogen

Description:...egion of JAB1 (Jun activation domain-binding protein 1 CSN5). Specificity: Specific for the JAB1 protein. Reactivity: Human (positive controls: 293T jurkat cell lysates). Applications: Western blotting Immunoprecipitation ELISA...
Company:Invitrogen

Anisomycin from Sigma-Aldrich

Description:...iting peptidyl transferase activity in eukaryote ribosomes. Reported to induce apoptosis in a variety of cells including promyelocytic leukemia cells, jurkat cells, ventricular myocytes, and colon adenocarcinoma cells. Initiates intracellular signals and immediate early gene induction. Selective signaling a...
Company:Sigma-Aldrich

Rabbit Anti-SKAP55 Polyclonal Antibody, Unconjugated from ABR-Affinity BioReagents

Description:...y used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~ 60 kDa protein from human jurkat cells.This antibody has also been used in immunoprecipitation and immunocytochemical procedures. PA1-525 antigen corresponds to amino acids 2-...
Company:ABR-Affinity BioReagents

BlockAid blocking solution *for use with microspheres* from Molecular Probes (Invitrogen)

Description:...ing of labeled microspheres. BlockAid blocking solution has been found to be effective in flow cytometry applications involving NIH 3T3, A431, RAW and jurkat cell lines, although less effective in HMC-1 cells. We expect BlockAid blocking solution to be useful for reducing the nonspecific binding of protein-...
Company:Molecular Probes (Invitrogen)

Jurkat in Biological Dictionary

Genomic DNA

...oratories. 100µg. 1mg/ml. Pricing Info. Request Info ... Human DNA (cytosine-5) Methyltransferase (Dnmt3A) Amino-terminal Ab ... 5-Aza-dc Treated jurkat Genomic DNA . CpG Methylated HeLa Genomic DNA ... ...