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Isoelectric in Medical Technology

Genetic Analysis Systems Enter the Clinical Mainstream

...uid Kit provides a sensitive and rapid method of separating and detecting oligoclonal immunoglobulin bands in unconcentrated cerebrospinal fluid using isoelectric focusing. The presence or absence of these bands is important as supporting evidence for the diagnosis of central nervous system (CNS) disorders. This...
Isoelectric in Medical Products

Agarose IEF

Description:... Agarose IEF is suitable for isoelectric focusing of large molecules and is the gel of choice for many IEF applications and immunochemical detection methods....
Company:GE Healthcare, formerly Amersham Biosciences

Proteomelab™ PA 800 Protein Characterization System

Description:... The ProteomeLab PA 800 resolves and quantifies proteins by their isoelectric point and molecular weight, generates high-resolution peptide maps and carbohydrate profiles and provides front-end separation to mass spectrometry....
Company:Beckman Coulter, Inc.
Isoelectric in Biological Technology

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

K. H. Valkonen*, N. Marttinen*, H.-L. Malinen*, V.-P. Jaakola** and T. Alatossava* * Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, FIN-88600 Sotkamo, Finland ** Department of Biological and Environmental Scie...

Protein Depletion for Plasma and Serum Proteomic Analysis

...rophoresis; CZE, Capillary Zone Electrophoresis; CIEF, Capillary isoelectric Focusing; CGE, Capillary Gel Electrophoresis. ...N: Low-molecular-weight human serum proteome using ultrafiltration, isoelectric focusing, and mass spectrometry. Electro phoresis 2004, 25 :129...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

...sion protein (TOBBLP4, gift from Dr. J. Denecke). 3 Preparative isoelectric Focusing Fractions 35, 36, and 37 from the Model 491 Prep Cell...ractions 3537 from the Model 491 Prep Cell run using preparative isoelectric focusing produced what appears to be a highly pure form of BiP i...

Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A

... were processed with the ReadyPrep 2-D cleanup kit to remove salts and detergents and to create a low-conductivity sample suitable for isoelectric focusing (IEF). This cleanup kit was also employed, if required, to concentrate proteins from dilute samples. Prior to IEF all s...

Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A

... of proteins. Methods Preparation of isoelectric Focusing Strips Cells from a human cell line were lysed with 7 ...nsistent with the exception of some minor differences due to the isoelectric focusing step. The highly expressed proteins did not suffer from...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

...y bound membrane proteins (E3). Samples were either subjected to isoelectric focusing immediately following extraction or stored at -80C unti...hydrated overnight in a humidity chamber. Once fully rehydrated, isoelectric focusing of proteins was carried out with the PROTEAN IEF cell u...

The Bio-Rad Silver Stain, Rev E

...hods 74, 229239 (1984) 9. Confavreux C et al., Silver stain after isoelectric focusing of unconcentrated cerebrospinal fluid: visualization of...atrick BA, Detection of oligoclonal bands in unconcentrated CSF: isoelectric focusing and silver staining, Neurology 33, 13651368 (1983) ...

Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

...to differences in protein staining efficiency. Unique protein characteristics, such as primary sequence, amino acid composition, isoelectric point, and presence of modified amino acids and prosthetic groups, can affect the levels of staining and colorimetric signals produced. ...

Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C

...thanks to custom software developed by Protein Databases, Inc., now owned by Bio-Rad. Figure 1 shows the synthetic master (IEF = isoelectric focusing) 2-D PAGE image of noncultured human keratinocyte proteins as depicted on the Web (http://biobase.dk/cgi-bin/celis). ...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

...n (TOBBLP4, gift from Dr. J. Denecke). 3 Preparative isoelectric Focusing Fractions 35, 36, and 37 from the Model 491 Prep Cell...fractions 3537 from the Model 491 Prep Cell run using preparative isoelectric focusing produced what appears to be a highly pure form of BiP in...
Isoelectric in Biological Products

PhastGel DNA Silver Staining Kit from GE Healthcare, formerly Amersham Biosciences

Description:...hastGel DNA Silver Staining Kit, 1. Select from homogeneous and gradient polyacrylamide gels.Separate proteins and peptides on denaturing, native, and isoelectric focusing gels.Save time and improve reproducibility with precast gels, buffer strips, and staining kits.Achieve detection sensitivity comparable to th...
Company:GE Healthcare, formerly Amersham Biosciences

PlusOne Dithiothreitol from GE Healthcare, formerly Amersham Biosciences

Description:...srupt cells and tissues.Clean up samples from contaminants.Efficiently dialyze small sample volumes. Accurately quantitate protein for first-dimension isoelectric focusing.Reagents specifically qualified for use in 2-D electrophoresis.High purity chemicals and reagents for protein solubilization. Category: Prote...
Company:GE Healthcare, formerly Amersham Biosciences

ProteoExtract Protein Precipitation Kit from Calbiochem

Description:...y in solution. Precipitated proteins can easily be re-suspended in the buffer of choice for a wide range of proteomics downstream applications such as isoelectric focusing (IEF), 2DGE, 1DGE or for tryptic digestion prior to mass spectrometry and peptide separation schemes. • The ProteoExtract™ Protein...
Company:Calbiochem

Rotofor Purification System With Power Supply, 220-240 V from Bio-Rad

Description:... The Rotofor purification system with PowerPac 3000 power supply, 220-240 V, is used for protein separation and purification by liquid-phase isoelectric focusing (IEF). The system includes both the mini (18 ml) and standard (60 ml) focusing chambers, chassis, harvesting apparatus, cooling finger, elect...
Company:Bio-Rad

Rotofor Purification System, 100-120 V from Bio-Rad

Description:... The Rotofor purification system, 100-120 V, is used for protein separation and purification by liquid-phase isoelectric focusing (IEF). The system includes both the mini (18 ml) and standard (60 ml) focusing chambers, chassis, harvesting apparatus, cooling finger, elect...
Company:Bio-Rad

UltraLink Immobilized NeutrAvidin from Pierce Biotechnology, Inc.

Description:... to provide a biotin-binding protein with exceptionally low nonspecific binding properties. NeutrAvidin does not contain carbohydrate and has an ideal isoelectric point (6.3) for the best utility in a variety of applications. For assay development, Pierce has coated NeutrAvidin onto microplates and pre-blocked ...
Company:Pierce Biotechnology, Inc.

PowerPac 3000 Power Supply 100/120 V from Bio-Rad

Description:...c 3000 power supply is the most versatile power supply available, ideal for isoelectric focusing, DNA sequencing, isotachophoresis, SDS-PAGE, 2-D electrophoresis, ...he PowerPac 3000 power supply offers enough power to run the most demanding isoelectric focusing experiments, or up to four DNA sequencing cells at the same time....
Company:Bio-Rad

Model 175 Tube Cell from Bio-Rad

Description:...t runs polyacrylamide tube gels for 2-D electrophoresis applications. The upper buffer chamber can accommodate twelve 4-8 mm OD tubes 9-23 cm long for isoelectric focusing (IEF) applications. The tube cell includes upper buffer chamber and cooling core, lower buffer chamber, lid with cables, grommets, stoppers t...
Company:Bio-Rad

Model 111 Mini IEF Cell, complete from Bio-Rad

Description:... The Model 111 mini IEF cell is an apparatus dedicated to general analytical isoelectric focusing (IEF) using polyacrylamide or agarose slab gels. The cell includes the electrophoresis chamber and lid, graphite electrodes, casting tray, 5 ...
Company:Bio-Rad

Chemical Inkjet Printer from Shimadzu Biotech

Description:...with leading proteomics company, ProteomeSystems. The strategy compliments established protocols in resolving proteins by 2-D gels (using the proteins isoelectric point and apparent molecular weight). However, unlike classical approaches the ChIP reproducibly dispenses picoliter volumes of reagents to defined lo...
Company:Shimadzu Biotech
Isoelectric in Biological Definition

Gel electrophoresis

... Gel electrophoresis is a group of techniques used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point . Gel Electrophoresis is usually performed for analytical purposes, but may be used as a preparative technique to partially purify molecules to...

Gel electrophoresis

... Gel electrophoresis is a group of techniques used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point . Gel Electrophoresis is usually performed for analytical purposes, but may be used as a preparative technique to partially purify molecules to...

Molecular biology

...teins can be separated based on size using an SDS-PAGE gel. Proteins can also be separated based on their electric charge , using what is known as an isoelectric gel... Western blotting and immunochemistry Main article: Western blot Antibodies to most proteins can be created by injecting small amou...

Proteome

...ly been practiced through the separation of proteins by two dimensional gel electrophoresis . In the first dimension, the proteins are separated by isoelectric focusing , which resolves proteins on the basis of charge. In the second dimension, proteins are separated by molecular weight using SDS-PAGE . T...

Proteomics

... Key technologies used in proteomics One and two-dimensional gel electrophoresis are used to identify the relative mass of a protein and its isoelectric point . X-ray crystallography and nuclear magnetic resonance are used to characterize the three-dimensional structure of peptides and protein...
Isoelectric in Biological Dictionary

Tubulin

...ad, the polymerized tubulin forms two-dimensional crystalline sheets that ... s of a- and ß- tubulin . These subunits are slightly acidic with an isoelectric point ... Tubulin was long thought to be specific to eukaryote. Eukaryote ... Drosophila has four ß- tubulin genes: each encodes a distinct is...
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