Preventing soil erosion in continuous corn
...nuously, in part because perennially high residue amounts favor annual harvests, and also because corn residue left on the soil surface is a source of inoculum
for corn diseases.
Removal of corn residue, however, may require changes in tillage for increased efficiency and protection against soil erosion. Y...
Humans fostering forest-destroying disease
...amplifying the diseases impact and spread.
A recent article published by Meentemeyer and colleagues in the Journal of Ecology showed that pathogen inoculum
load (the actual amount of infectious pathogen present) is greater in forests with high landscape connectivity and high abundance of host species. In...
Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
...its ability to obtain vital nutrients and oxygen for the production of rapamycin.
Materials and Methods
of Streptomyces hygroscopicus NBS-9746, selected from ATCC 29253 cell strain, was cultured in an Innova
4300 incubator shaker, then transferred to a...
Fundamentals of Fermentation: Techniques For Benchtop Fermentors
port terminus using aseptic techniques, and then the clamps are opened to allow for
addition. Once the material is all in (except for any residual inoculum
which must be
retained for testing) the clamps are secured and the shake flask is disconnected. At this
point, the harvest port terminus must be c...
Glossary for The BioPharm Guide to Fermentation and Cell Culture
batch culture Large-scale cell culture in which cell inoculum
is cultured to a
maximum density in a tank or airlift fermentor, harveste...y.
inoculate To introduce cells into a culture medium.
Material (usually cells) introduced into a culture medium.
...can be described as follows:
* Stage 1: inoculum
* Stage 2: inoculum
* Stage 3: fermentor culture
STAGE 1: inoculum
The objective ...
Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System
... Once youve verified that your cell line will grow using
FibraCel disks, inoculate one to four FibraStage
bottles, as desired. Begin with an inoculum
of at least
1 2 x 10 8 cells per bottle for mammalian cell cultures,
and 1.5 2 x 10 8 cells per bottle for insect cell cultures.
(See Table ...
Hybridoma Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor
Inoculate 500 ml of inoculum
suspension to provide a starting cell count of 2.4...om Hyclone and 0.1% Pluronic from Gibco.
Hybridoma cell line 3G5 from ATCC
was cultivated in an NBS Shaker (Innova 2000), w...
Insect Cell Culture Using a BioFlo 110 Benchtop Bioreactor
...FX-Insect (liquid) medium supplied by Hyclone
(Logan, UT) (serum-free expression medium).
Hi-5 insect cell inoculum
supplied by Hyclone (Logan, UT)
Inoculum was cultivated in a NBS Shaker (Innova 2000)
which was inside an Incubator (Fisher Scientific, Model No...
E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
...ials and Methods
was prepared using LB broth at a 25-g/L
concentr.... OD 600 nm was 5.50 at the time
of inoculation. inoculum
volume was 5% of the 5 L
working volume, or 250 ...
P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
the methanol feed-pump.
was prepared using Pichia shake-flask
growth m... they are heat-sterilized and cooled.
was cultivated for 40 hours at 28C in a rotary