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Immunostaining in Medical News

BioMosaics' Glypican-3 Antibody (clone 1G12) Validated at PhenoPath Laboratories for Immunohistochemical Detection of Liver Cancer

...d B0025), or as a ready-to-use, pre-diluted product for IHC (Catalog # B0134). Both formats are suitable for use with manual protocols or in automated immunostaining instruments. The antibody validation studies were performed with formalin fixed paraffin embedded tissue sections used in routine immunohistoche...

Can pathological techniques help identify primary colorectal signet ring cell carcinoma?

...empted to differentiate between a gastric and colonic primary. Both specimens were positive for cytokeratins 7 (CK 7) and cytokeratins 20 (CK 20). immunostaining profiles for CK 7 and CK 20 have been used to characterize and differentiate signet ring cell carcinoma of breast, stomach and colon. It has been sugg...

What are simple ways to judge the efficacy of 5-fluorouracil in colonic neoplasm?

...08 in the World Journal of Gastroenterology addresses this question. Immunoreactivity score (IRS) is a semiquantitative analysis for detecting the immunostaining results. Dr.Wei-Xing Wang and his colleagues used monoclonal antibody TS106 to detect the TS protein in the paraffin-embedded specimens. The samples c...
Immunostaining in Medical Products

Shandon Sequenza Immunostaining Center

Description: The Shandon Sequenza uses the Shandon Coverplate system which ensures consistency and excellent staining quality. Allows the user to perform large immunostaining runs (up to 50 slides/run) giving excellent reproduction consistency....
Company:ThermoFisher Scientific

Shandon Sequenza® Slide Rack

Description:...za Slide Rack works hand in hand with the Sequenza immunostaining center offers you an organized, reliable method for immunostaining. The Sequenza immunostaining center uses the Coverplate system which ensures c...nsiderable tech time for other tasks. The Sequenza immunostaining center offers substantial reagent savings in both ...
Company:ThermoFisher Scientific

Shandon Humidity Chamber

Description:... The humidity chamber maintains a moist environment during immunostaining procedures as well as other laboratory procedures requiring controlled incubation steps....
Company:ThermoFisher Scientific
Immunostaining in Biological News

Creating ideal neural cells for clinical use

...onolayers. Immunochemical and RT-PCR analysis of the cells demonstrated that they were uniformly C-NPCs. Whole-genome analysis confirmed this finding. immunostaining and imaging showed that the cells could be differentiated into three distinct types of neural cells. The team then demonstrated that the C-NPCs differ...

Origin of cells for connective tissues of skull and face challenged

...and connective tissue of the head and face." In the mouse embryo at eight days gestation, Weston and collaborators used high-resolution imaging and immunostaining techniques to identify and track the dispersal of cells known to jump start connective and skeletal tissue development. They were able to see clearly ...

New technique will produce a better chromosome map

...nomical than electron microscopy, with comparable or superior results. With a more accurate chromosome map, researchers will next use fluorescent immunostaining of proteins that bind to specific DNA sequences. These landmarks will help them tease out the relationship of the sequence to the physical structure. ...

Making a face: A new and earlier marker of neural crest development

...according to a recent study published in the journal Nature. The collaborators at Yale and Caltech demonstrate with three different technologies -- immunostaining of proteins, in situ hybridization and multiplex RT-PCR of mRNAs -- that formation of neural crest cells in chick embryos is independent of both mesod...
Immunostaining in Biological Technology

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...taker Molecular Applications) ◊ immunostaining jars RNase-free Technique ...RNase Inhibitor to the antibody cocktails during immunostaining is recommended as it prevents RNA degradation dur...RNA. To overcome this limitation, a modified rapid immunostaining protocol was developed for macrophage-specific CD6...

Biolistic Transfection of Organotypic Brain Slices

... from the enzyme), we visualized these transfected neurons using immunostaining to locate β-galactosidase (Lo et al., 1994; primary antibod... from Boehringer-Mannheim). Figure 4 illustrates how effectively immunostaining increases the resolution of the fine processes of the neurons in...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

...encer 4.1-CMV puro vectors engineered to express an siRNA targeting GAPDH mRNA (CMV-Gap) or a scrambled sequence (CMV-Scr). (A) immunostaining of HeLa cells two days after transfection showing reduction of GAPDH levels in cells transfected with CMV-Gap. Green: GAPDH prot...

Drive Long-Term Silencing with CMV Vectors

... Clones that showed low levels of GAPDH protein expression also showed low levels of GAPDH mRNA expression. (B) Representative immunostaining data showing the relative fluorescence differences between a clone having GAPDH reduced and another clone that did not have a si...

hNT BlueNeurons Express b-Galactosidase at High Levels

...an be identified in transplanted tissue by staining with antibodies specific for human markers, an additional method of identification would allow immunostaining for multiple markers. The lacZ gene was chosen as a marker to be introduced into hNT neurons because it is very stable and does not appear to inte...

Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression

...test staining procedures and reagents. We report our results using Complete View array slides for hematoxylin and eosin staining of murine tissue, immunostaining of murine tropomyosin, and labeling RNA of murine salivary gland. These tests confirm that Complete View tissue array slides ensure reliable scree...
Immunostaining in Biological Products

Ultrastreptavidin (USA) Horseradish peroxidase (HRP) 150 Test, Murine, Detection Kit (SIGNET) from Covance Research Products, Inc

Description:... Signets unique formulation of ultra Streptavidin to provide sensitivity, specificity and reliablility. Signet reagents will achieve excellent quality immunostaining in both manual and automated protocols. Signets USA Detection reagents set an industry standard for quality. Reagents are available in several dif...
Company:Covance Research Products, Inc

Ultrastreptavidin (USA) Horseradish peroxidase (HRP) 500 Test, Rabbit, Detection Kit (SIGNET) from Covance Research Products, Inc

Description:... Signets unique formulation of ultra Streptavidin to provide sensitivity, specificity and reliablility. Signet reagents will achieve excellent quality immunostaining in both manual and automated protocols. Signets USA Detection reagents set an industry standard for quality. Reagents are available in several dif...
Company:Covance Research Products, Inc

Ultrastreptavidin (USA) Horseradish peroxidase (HRP) 1000 Test, Multi-Species, DAB Detection Kit (SIGNET) from Covance Research Products, Inc

Description:... Signets unique formulation of ultra Streptavidin to provide sensitivity, specificity and reliablility. Signet reagents will achieve excellent quality immunostaining in both manual and automated protocols. Signets USA Detection reagents set an industry standard for quality. Reagents are available in several dif...
Company:Covance Research Products, Inc

Ultrastreptavidin (USA) Horseradish peroxidase (HRP) 50 Test, Murine, DAB Detection Kit (SIGNET) from Covance Research Products, Inc

Description:... Signets unique formulation of ultra Streptavidin to provide sensitivity, specificity and reliablility. Signet reagents will achieve excellent quality immunostaining in both manual and automated protocols. Signets USA Detection reagents set an industry standard for quality. Reagents are available in several di...
Company:Covance Research Products, Inc

Ultrastreptavidin (USA) Horseradish peroxidase (HRP) 50 Test, Multi-Species, AEC Detection Kit (SIGNET) from Covance Research Products, Inc

Description:... Signets unique formulation of ultra Streptavidin to provide sensitivity, specificity and reliablility. Signet reagents will achieve excellent quality immunostaining in both manual and automated protocols. Signets USA Detection reagents set an industry standard for quality. R eagents are available in several di...
Company:Covance Research Products, Inc

Ultrastreptavidin (USA) Horseradish peroxidase (HRP) 50 Test, Murine, AEC Detection Kit (SIGNET) from Covance Research Products, Inc

Description:... Signets unique formulation of ultra Streptavidin to provide sensitivity, specificity and reliablility. Signet reagents will achieve excellent quality immunostaining in both manual and automated protocols. Signets USA Detection reagents set an industry standard for quality. Reagents are available in several di...
Company:Covance Research Products, Inc

B-Phycoerythrin Labeling Kit-NH2 from Abnova Corporation

Description:... B-Phycoerythrin Labeling Kit - NH2 is primarily used for the preparation of B-phycoerythrin-labeled antibody for immunostaining and cellular proteins for tracing. NH2-Reactive B-Phycoerythrin, a component of this kit, has succinimidyl group (NHS), and can easily make a covalent...
Company:Abnova Corporation

GyroTwister GX-1000 3-D Shaker from MIDSCI

Description:... x 30 cm platform and tube mat are supplied with the shaker. Gyratory action provides gentle yet thorough mixing Hybridization, staining gels, immunostaining and general mixing Safe for use in temperature controlled environments Double platform and larger platforms available ...
Company:MIDSCI
Immunostaining in Biological Dictionary

Spermatids

... Rodent spermatocytes, round and elongated spermatids (14–16), and Sertoli (13) ... ( 14) appeared to demonstrate that spermatids had more intense immunostaining and ... Caenorhabditis elegans spermatids complete a dramatic morphogenesis to crawling ... Spermatids from males activate soon after they ar...

Carboxyl Terminus

...... carboxyl terminus is highly mobile in its GDP-bound ... ... occurring dystrophin carboxyl terminus , Dp-71, restores the sarcolemmal ... immunostaining for the carboxyl terminus of dystrophin was performed to ... A novel apoptosis pathway activated by the carboxyl terminus of p21 ... depe...
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