Immunofluorescence

CRi's New Vectra(TM) Platform Rapidly Analyzes Tissue Slides and Microarrays in Brightfield and Fluorescence

... images from samples stained with standard H&E, immunohistochemical and immunofluorescence techniques. The Vectra system utilizes CRi's inForm(TM) Advanced Image...tra has allowed us to break the boundary of routine immunohistochemical and immunofluorescence studies by supporting multiplexed staining and analysis to reveal the under...

Frost & Sullivan Lauds Aesku.Diagnostics For Providing an Overall Analysis Platform For a Wide Range of Autoimmune Complications

...shelf life of up to 24 months with a production capacity of nearly 500 plates per hour. The AESKUSLIDES(R) brand encompasses a broad portfolio of immunofluorescence slides. All these slides are vacuum-sealed for longer shelf life as well as stability, and use a highly hydrophobic Teflon mask to avoid sample cross ...

What is the effect of fluoxetine on mast cell?

... They found that depression induced mast cell proliferation, activation, and granule hyperplasia. Compared with the normal control group, the average immunofluorescence intensity of gastric antrum rMCP-1 significantly increased in depressed model group (37.4 7.7 vs 24.5 5.6, P These findings will conduce to unde...

Does HBV infection induce acute cellular DNA damage?

... Using HL7702 hepatocytes with HBV-positive serum as material, they evaluate protein expression levels in HBV infected cells or in non-infected cells; immunofluorescence to show ATR foci ands Chk1 phosphorylation foci formation; flow cytometry to analyze the cell cycle and apoptosis; ultraviolet radiation (UV) and ioni...

Sigma-Aldrich and Atlas Antibodies Announce the Release of 2,000 New, Highly-Validated Prestige Antibodies

...y is accompanied by 576 tissue immunohistochemistry (IHC) images and 118 cell and cell line immunocytochemistry (ICC) images. These images, as well as immunofluorescence (IF) and western blotting (WB) data, are publicly accessible via the Human Protein Atlas ( http://www.proteinatlas.org ). Prestige Antibodies are opti...

Researchers Find Out The Cause Of Immune Attacks Behind Hearing Loss

...erienced improvement in their hearing after steroid treatment, and 35 did not. But the vast majority, 89 percent, of those who improved had a positive immunofluorescence test for an antibody to IESCA, said the researchers. The results strongly suggest that a direct test for antibodies could accurately predict which pat...

Immunofluorescence in Medical Technology

Quest Diagnostics Introduces First Commercial Laboratory Test for Identifying the H1N1 Swine Flu Virus

...cus Diagnostics reference laboratory, perform a broad range of testing services to detect Influenza viruses, including PCR, rapid cell culture, direct immunofluorescence (DFA) and enzyme immunoassay (EIA) techniques. Depending on the laboratory, the company can perform testing on different types of collected specimens,...

Point of Care Cardiac Analyzers Yield Rapid Cardiac Marker Results

...sponse Biomedical Corp. offers the RAMP Cardiac Marker System, with separate test kits for CK-MB, myoglobin and troponin I. It is also a quantitative immunofluorescence assay for EDTA whole blood specimens. To perform the test, a diluted blood sample is added to the sample well of the test cartridge, the red blood ce...

Rapid Flu Tests May Prove Especially Helpful This Season

...ilable for influenza include reliable lab-based detection based on viral culture, serology, rapid antigen testing, polymerase chain reaction (PCR) and immunofluorescence testing. All are time-consuming, however, in a year when time is especially important. Fortunately, three medical firms produce quick-results k...

Immunofluorescence in Medical Products

Skin Immunofluorescence

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

Anti-Thyroid Antibody (TA) Test Kit - Monkey Thyroid Substrate

Description:... Inverness Medical Professional Diagnostics is a leading provider of immunofluorescence assays, with an extensive IFA and DFA test menu that includes kits for autoimmune diseases, infectious diseases and STDs as well as miscellaneous conj...
Company:Inverness Medical Innovations

Autoantibody Screen (AAS) Test Kit - Rat Kidney/Stomach Substrate

Description:... Inverness Medical Professional Diagnostics is a leading provider of immunofluorescence assays, with an extensive IFA and DFA test menu that includes kits for autoimmune diseases, infectious diseases and STDs as well as miscellaneous conj...
Company:Inverness Medical Innovations

Anti-Nuclear Antibodies (ANA) Hep-2 Substrate

Description:... Inverness Medical Professional Diagnostics is a leading provider of immunofluorescence assays, with an extensive IFA and DFA test menu that includes kits for autoimmune diseases, infectious diseases and STDs as well as miscellaneous conj...
Company:Inverness Medical Innovations

Anti-Smooth Muscle Antibody (ASMA) Test Kit

Description:... Inverness Medical Professional Diagnostics is a leading provider of immunofluorescence assays, with an extensive IFA and DFA test menu that includes kits for autoimmune diseases, infectious diseases and STDs as well as miscellaneous conj...
Company:Inverness Medical Innovations

Anti-Mitochondrial Antibody (AMA) Test Kit

Description:... Inverness Medical Professional Diagnostics is a leading provider of immunofluorescence assays, with an extensive IFA and DFA test menu that includes kits for autoimmune diseases, infectious diseases and STDs as well as miscellaneous conj...
Company:Inverness Medical Innovations

Anti-Native Deoxyribonucleic Acid (nDNA) Test Kit

Description:... Inverness Medical Professional Diagnostics is a leading provider of immunofluorescence assays, with an extensive IFA and DFA test menu that includes kits for autoimmune diseases, infectious diseases and STDs as well as miscellaneous conj...
Company:Inverness Medical Innovations

Fluorescent Treponemal Antibody Absorption (FTA-ABS) Test Kits

Description:... Inverness Medical Professional Diagnostics is a leading provider of immunofluorescence assays, with an extensive IFA and DFA test menu that includes kits for autoimmune diseases, infectious diseases and STDs as well as miscellaneous conj...
Company:Inverness Medical Innovations

Toxoplasma gondii (Toxo) Antibody IgM

Description:... Inverness Medical Professional Diagnostics is a leading provider of immunofluorescence assays, with an extensive IFA and DFA test menu that includes kits for autoimmune diseases, infectious diseases and STDs as well as miscellaneous conj...
Company:Inverness Medical Innovations

Mycoplasma Pneumoniae (IgM)(Crown Titer Substrate)

Description:... Inverness Medical Professional Diagnostics is a leading provider of immunofluorescence assays, with an extensive IFA and DFA test menu that includes kits for autoimmune diseases, infectious diseases and STDs as well as miscellaneous conj...
Company:Inverness Medical Innovations

Immunofluorescence in Medical Definition

Hematopathology

...thology - Forensic Pathology - Dental pathology Gross examination - Histopathology - Immunohistochemistry - Electron microscopy - immunofluorescence - Fluorescent in situ hybridization Clinical pathology Clinical chemistry - Hematopathology - Transfusion medicine - Medica...

Pathology

...thology - Forensic Pathology - Dental pathology Gross examination - Histopathology - Immunohistochemistry - Electron microscopy - immunofluorescence - Fluorescent in situ hybridization Clinical pathology Clinical chemistry - Hematopathology - Transfusion medicine - Medica...

Immunofluorescence in Medical Dictionary

Immunofluorescence

Immunofluorescence is the labeling of antibodies or antigens with fluorescent dyes. This technique is often used to visualize the subcellular distribution of biomolecules of interest. Immunofluorescent-labeled tissue sections... immunofluorescence n. Any of various techniques that use ant...

Tick fever

...ck fever is an acute viral infection spread by the bite of the ... immunofluorescence for Colorado tick fever -- will be positive if the person has ... ....ck fever is an acute viral infection spread by the bite of the ... immunofluorescence for Colorado tick fever -- will be positive if the person has ... ...

Colorado tick fever

... viral infection spread by the bite of the ... immunofluorescence for Colorado tick fever -- will be positive ... viral infection spread by the bite of the ... immunofluorescence for Colorado tick fever -- will be positive ...al infection transmitted by the bite of the ... immunofluorescence for Colorado tick fever -- will be positive ...

Immunofluorescence in Biological News

Exhibition showcases the 'art of science'

...e image. Third Prize went to Maria Ciocca, a 2005 alumna now at the University of Pennsylvania School of Medicine, for Worm Love, an image taken using immunofluorescence microscopy. The jurors for the top prizes were Shirley M. Tilghman, president of Princeton University and a renowned molecular biologist; the inte...

Proteins strangle cell during division

...lants, fungi, etc). Strangler proteins Until now little was known about the proteins that control cell division in the Archaea. With the use of immunofluorescence the researchers determined the location of these proteins in the cell and in doing so discovered that three proteins play a crucial role in the cell d...

New Lab Research May Help Those Deafened By Immune System Attack

...ot. But the vast majority, 89 percent, of those who improved had a positive immunofluorescence test for an antibody to IESCA that we have studied at U-M for years," says ... patients and 20 normal controls with two tests: a Western blot test and an immunofluorescence (IF) test based on KHRI-3. They correlated the results of those two tests w...

Immunofluorescence in Biological Technology

Sigma-Aldrich and Atlas Antibodies Announce 2,300 New, Highly Validated Prestige Antibodies(RM)

...Each Prestige Antibody is also associated with over 500 tissue immunohistochemistry (IHC) images, over 100 cell/line immunocytochemistry (ICC) images, immunofluorescence (IF) and western blotting (WB) data, all publicly accessible via the Human Protein Atlas ( www.proteinatlas.org ). In August 2008, Sigma-Aldric...

Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System

...r/ ligand binding4, apoptosis5, immunofluorescence5, and bead-based fluorescent immunoassays (FLISAs) 3. In the experiments discussed here, immunofluorescence and FLISA assays were combined in a single well, and the secreted cytokines and cell surface expression of an inducible marker were quantitated simult...

Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C

...s about protein expression profiles and immunofluorescence patterns that may be associated with ba... is indicated with small arrows, showed immunofluorescence patterns that were clearly different fr..., we show in Figures 4A, D, G, J, and M immunofluorescence pictures of cryostat sections from norm...

Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit

... control protein, Pab 1, was not affected (Figure 1A). The specific silencing of hrp48 and U2AF 50 proteins was also confirmed by immunofluorescence microscopy (data not shown). Direct observation of the Cy3 fluorescence demonstrated that both hrp48 and U2AF 50 labeled dsRNA ...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

...xpression 48 hr post-transfection using immunofluorescence microscopy (Figure 1A). The results clearly demons...s were analyzed for c-myc protein expression using immunofluorescence as described in Materials and Methods. ...ced in only 2 or 3 days. Supporting immunofluorescence data showed that the siRNA was cent...

Using Validated siRNAs in Functional Genomic Assays

...ch they were transfected and transferred to wells of a 96 well plate. Following overnight recovery, the cells were processed for immunofluorescence staining of NF-kappaB. There are several distinct signaling pathways that can trigger the nuclear translocation of NF-kappaB. T...

The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis

...NA Inhibitors (Ambion) or a negative control miRNA inhibitor (CD). Three days post-transfection, RAS expression was monitored by immunofluorescence (A, C) and quantified using MetaMorph software (Universal Imaging corporation) (B, D; triplicate assays). Modified with permissi...

Superior Gene Silencing Using Adenoviral Vectors

...plementary to the wild type construct, whereas that of Ad-C10i was complementary to the mutant construct. (A) Green: GFP. Red: immunofluorescence of the polyQ construct detected with 1C2 antibody. Ad-G10i reduced wt ataxin-3 levels 95% but did not affect mutant ataxin-3 lev...

RNAi: A How To for New Users

...and its effects can be easily monitored by qRT-PCR or other methods at the mRNA level, or by Western blot or immunofluorescence at the protein level. In fact, Ambion provides a highly specific antibody for measuring these protein level ...

RNAi in Non-Mammalian Cultured Cells

...rotein, Pab 1, was not affected (Figure 3A). The specific silencing of hrp48 and U2AF 50 proteins was also confirmed by immunofluorescence microscopy (data not shown). Direct observation of the Cy3 fluorescence demonstrated that both hrp48 and U2AF 50 labele...

Immunofluorescence in Biological Products

Terminal deoxynucleotidyl transferase (TdT) Immunofluorescence Assay Kit from AbD Serotec

Description: Terminal deoxynucleotidyl transferase (TdT) is an intracellular marker normally present at high levels in cortical thymocytes and minor subpopulation of bone marrow prelymphocytes. Elevated levels of the enzyme and increased numbers of TdT cells are found to be associated with some lymphoblastic le...
Company:AbD Serotec

Anti-Varicella-zoster Virus, Nucleocapsid Protein Monoclonal Antibody, Unconjugated from CHEMICON

Description:... Recognizes the VZV nucleocapsid by indirect immunofluorescence and immunoprecipitation. Shows cross-reactivity to HSV....
Company:CHEMICON

LIGHT DIAGNOSTICS™ CMV pp65 Dextran Leukocyte Separation Kit, included in kit #3247 from CHEMICON

Description:... The Light Diagnostics Cytomegalovirus (CMV) pp65 Antigenemia immunofluorescence Assay (IFA) is intended for the qualitative identification of lower matrix protein pp65 of CMV in isolated peripheral blood leukocytes. Kit Component...
Company:CHEMICON

Rabbit Anti-PKCZeta (C-term) Monoclonal Antibody,, Clone ZMD.330 from Invitrogen

Description:... mouse and rat kidney and brain homogenates). Based on sequence homology reactivity with Human and rabbit is expected. Applications: Western blotting immunofluorescence Immunohistochemistry...
Company:Invitrogen

Michel's Transport Wash Buffer from ScyTek Laboratories

Description:...r Michels Transport Fluid (cat.# MTF). Michels Transport Fluid is useful for transportation of specimens (such as renal biopsies and lymph nodes) for immunofluorescence studies. Samples may remain in Transport Fluid for up to 5 days at room temperature. Michels Transport Fluid is not a fixative and is not suitable f...
Company:ScyTek Laboratories

Fluorescent Mounting Media from KPL

Description:... Glycerol-based mounting medium for immunofluorescence microscopy. Formulated to retard photobleaching of fluorochromes such as fluorescein and rhodamine. Product can also be used as a diluent for fluores...
Company:KPL

ApopTag Red In Situ Apoptosis Detection Kit from CHEMICON

Description:...etects apoptosis in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody with a rhodamine fluorochrome. The kit provides indirect immunofluorescence staining for 40 samples. Results are analyzed using either fluorescence microscopy or flow cytometry (requires a special laser and filters for rhodami...
Company:CHEMICON

ApopTag Fluorescein In Situ Apoptosis Detection Kit from CHEMICON

Description:... situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule. It provides indirect immunofluorescence staining for 40 samples. Results are analyzed using either flow cytometry or fluorescence microscopy....
Company:CHEMICON

Mouse Anti-GST Tag Monoclonal Antibody, Unconjugated from Novagen

Description:...n proteins containing the GSTTag sequence expressed in E. coli , yeast, mammalian, and in vitro transcription/translation systems by Western blotting, immunofluorescence or immunoprecipitation. GSTTag fusion proteins can be efficiently expressed using Novagens pET-41 or pET-42 vector series....
Company:Novagen

Immunofluorescence in Biological Definition

Monoclonal antibody

... be used to detect for the presence and quantity of this substance, for instance in a Western blot test (to detect a substance in a solution) or an immunofluorescence test (to detect a substance in a whole cell). Monoclonal antibodies can also be used to purify a substance with techniques called immunoprecipitation...

Immunofluorescence in Biological Dictionary

Medulla oblongata

...edulla oblongata is the lower half of the brainstem. ... This stops involuntary actions controlled by the medulla oblongata . ... In a double immunofluorescence experiment, this sagittal section of rat brain featuring the medulla oblongata was fixed, permeabilized, blocked with 10-percent ... Definition...

Giemsa stain

...for cytology and microorganisms. ... Although the giemsa stain is a powerful tool, it rarely is ... The three tests include Giemsa stain , immunofluorescence assay (IFA), and PCR. ... Although the Giemsa stain did not pick up any case that was missed by IFA or PCR, ... The application of Giemsa tec...

Acrosome reaction

... movements. ... The acrosome reaction is a necessary prerequisite for spermatozoa to acquire ... Scoring of the acrosome reaction by direct immunofluorescence and flow cytometry ... Sea Urchin Sperm Acrosome Reaction ... various stages of the acrosome reaction in sea urchin sperm. The acrosome ...