Immunoblotting

Study helps explain fundamental process of tumor growth

...to cancer cells. Reasoning that tumor tissue switches pyruvate kinase expression from an adult M1 isoform to the embryonic M2 isoform, they performed immunoblotting and immunohistochemistry analysis of numerous cancer cell lines, breast cancer models and human colon cancer, confirming that PKM2 was the only form o...

Autism's origins: Mother's antibody production may affect fetal brain

...other to child during pregnancy could interfere with the fetal brain directly. To test their hypothesis, the research team used a technique called immunoblotting (or Western blot technology), in which antibodies derived from blood samples are exposed to adult and fetal brain tissue to check whether the antibodi...

Immunoblotting in Biological Technology

Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A

... series of experiments, up to 10,000 sporocysts were bombarded. The expression of GFP was verified by RT-PCR (results not shown) and immunoblotting (Figure 3). Confocal laser scanning microscopy revealed GFP expression within the larvae after biolistic treatment at 900 psi (F...

Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C

... et al. 1991), which is synthesized by some differentiated cells in SCCs and that can be detected in the urine of SCC patients using immunoblotting in combination with enhanced chemiluminescence detection (Celis et al. 1996b, Rasmussen et al. 1996, stergaard et al. 1997). Wit...

Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A

... 7. Tom, T., Hickey, R. J. and Malkas, L. H., Identification of multiprotein complexes containing DNA replication factors by native immunoblotting of HeLa cell protein preparations with T-antigen dependent SV40 DNA replication activity, J. Cell. Biochem., 63 , 259267 (1996)...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

... SDS-PAGE, isoelectric focusing (IEF), and immunoblotting (IB). ANALYTICAL ELECTROPHORESIS ...estained as described above. immunoblotting The two βLG fractions, from prep... with polyclonal antibodies to bovine βLG. immunoblotting shows that βLG A/B and both eluted...

Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C

...horesis, 200 l aliquots of every fifth eluted fraction were TCA-precipitated and analyzed by Coomassie Blue-stained SDS-PAGE and immunoblotting for the presence of p23-DHFR (Figure 2). As confirmed by high-sensitivity immunoblot analysis, the protein preparation was purified ...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

...N-protein and epitopes A and D of the S-protein were confirmed by immunoblotting using porcine hyperimmune antiserum to TGEV (Stott, 1989) or a ...., 58 , 242250 (1997). 6. Stott, D. I., immunoblotting and dot blotting, J. Immunol. Methods, 119 , 153187 (1989). ...

TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins

...were incubated with gT-YC, gT-YD, gT-YE, and gT-F3 purified from BL21 or TKB1; specifically associated proteins were analyzed by immunoblotting with SHC specific antisera (Figure 3). SHC did not interact with fusion proteins produced in the BL21 strain, yet eff...

Highly Active and Removable AffinityTagged Lambda Phosphatase

... As positive controls, 500 ng (lane 4) and 100 ng (lane 7) of autophosphorylated EGFRKD were loaded onto the gel and detected by immunoblotting with an antibody to phosphotyrosine, the PY20 monoclonal antibody. figure 2 After ...

Immunoblotting in Biological Products

PARP Cleavage Immunoblotting Assay Kit from Upstate

Description: Kit Components: Anti-PARP LumiGLO Chemiluminescent Substrate Immunoblot Blocking Reagent Pyronin Y Reagent Goat Anti-Rabbit IgG, HRP conjugate Double-It Reagent Film Exposure Folders Autorad Orientation Markers ...
Company:Upstate

Rabbit Anti-Human Rab3A Polyclonal Antibody, Unconjugated from Assay Designs/Stressgen Bioreagents

Description:...s an ~25 kDa band, corresponding to the expected molecular mass of Rab3A, on immunoblots. The antibody specificity is confirmed in peptide inhibition immunoblotting studies....
Company:Assay Designs/Stressgen Bioreagents

Immun-Blot PVDF Sandwiches, 7 x 8.5 cm, 20 from Bio-Rad

Description:... filter paper that are preassembled into a blotting membrane and filter paper sandwich. These 7 x 8.5 cm membrane sandwiches for high binding capacity immunoblotting fit Ready Gel-sized gels and are supplied as a pack of 20....
Company:Bio-Rad

Mouse Anti-TATA Binding Proteins (TBP) Monoclonal Antibody, Unconjugated, Clone ITBP20 from Covance Research Products, Inc

Description:...he activity of TBP (prepared either as a nuclear extract or as a minimal activity extract) in a DNA transcription assay. This antibody is effective in immunoblotting and DNA supershift assays. *Predicted MW = 38 kD....
Company:Covance Research Products, Inc

In Situ Pan-Caspase Assay Kit from CHEMICON

Description:... residue is the target for cleavage, which occurs at the carbonyl end of the aspartic acid residue. Caspases can be detected via immunoprecipitation, immunoblotting techniques using caspase specific antibodies, or by employing fluorochrome substrates which become fluorescent upon cleavage by the caspase....
Company:CHEMICON

In Situ Pan-Caspase Assay Kit, Sulforhodamine from CHEMICON

Description:... residue is the target for cleavage, which occurs at the carbonyl end of the aspartic acid residue. Caspases can be detected via immunoprecipitation, immunoblotting techniques using caspase specific antibodies, or by employing fluorochrome substrates which become fluorescent upon cleavage by the caspase....
Company:CHEMICON

Rabbit Anti-Nonmuscle Myosin II Heavy Chain B (MHC-B) Polyclonal Antibody, Unconjugated from Covance Research Products, Inc

Description:...aised against the peptide SDVNETQPPQSE corresponding to the C-terminus of nonmuscle myosin heavy chain isoform B (MHC-B) This antibody is effective in immunoblotting and immunofluorescence. *Predicted MW = 200 kD....
Company:Covance Research Products, Inc

Rabbit Anti-Matrix Metalloproteinase-2, Hinge Region Antibody, Unconjugated from Sigma-Aldrich

Description:... Anti-Matrix Metalloproteinase-2, Hinge Region By immunoblotting the antibody reacts against the reduced and native protein. It reacts with free MMP-2 or MMP-2 bound to TIMP-2. affinity isolated antibody buffered a...
Company:Sigma-Aldrich

Mouse Anti-Human Angiogenin Monoclonal Antibody, Unconjugated, Clone 14017.7 from Sigma-Aldrich

Description:... from a 0.2 μm filtered solution in phosphate buffered saline. Titertest method: ELISA (c). WB. Titertest values: capture ELISA 1-2 μg/mL immunoblotting 1-2 μg/mL...
Company:Sigma-Aldrich

PARP Cleavage Detection Kit from Calbiochem

Description:...Contents: A control blocking peptide, uninduced immunoblotting standard, induced immunoblotting standard, PARP immunoblotting standard, and a user protocol....
Company:Calbiochem

Immunoblotting in Biological Definition

Immunology

...ugated with a radiolabel, fluorescent label, or color-forming enzyme and are used as a "probe" to detect it. Well known applications of this include immunoblotting , ELISA and immunohistochemical staining of microscope slides. The speed, accuracy and simplicity of such tests has led to the development of rapid ...

Immunoblotting in Biological Dictionary

Intercalated disc

...sts that the intercalated disc was saturated with cadherin protein in ... Stacked images of intercalated discs were traced with IPLab ... immunoblotting of intercalated disc -associated proteins in ... intercalated disc ... The nexus in the intercalated disc of the canine heart: quantit...