Addition of imidazole during binding improves purity of histidine-tagged proteins
K. Hölscher, M. Richter-Roth, and B. Felden de Neumann
GPC Biotech AG, Martinsried, Germany
The purity of histidine-tagged proteins* purified by metal chelate affinity chromatography can often be improved by optimizing the imidazole
concentration in the ...
Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system
... JNK ), and p38 MAPK , in mammalian cells (9). Also included were wells containing both 100-µM SB203580 plus anisomycin. SB203580 is a pyridinyl imidazole
MAPK inhibitor, reported to selectively inhibit p38 MAPK activity with little or no affect on JNK, ERK, or several other protein kinases (11). Plates...
New gravity-flow column gives faster and simpler purification of histidine-tagged proteins
... convenience by providing phosphate buffer concentrates and highly pure 2 M imidazole
stock solutions. This kit eliminates time-consuming manual buffer preparati...fluorescent protein (GFP-[His] 6 ; Mr 28 000) from an E. coli lysate. The imidazole
concentration in the sample and binding buffers was 45 mM for His GraviTrap...
Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
...ith either 75, 100, 250, 500, or 750
in the elution buffer. Following elution and a sub...kD His-tagged protein was then eluted with 5 CV of imidazole
buffer, followed by an addition...d with the 75, 100,
250, 500, and 750 mM imidazole
elution buffers were
superimposed using ...
Imidazole in Biological Products
Imidazole in Biological Definition
... bound to it. Histidine is a good ligand for nickel so the fusion protein will bind to the column. The protein can be eluted with a solution of imidazole
The his tag can also be used in detection of the protein via anti-his-tag antibodies . This can be useful in localization , ELIS...
Purine is a heterocyclic aromatic organic compound , consisting of a pyrimidine ring fused to an imidazole
The general term purines also refers to substituted purines and their tautomers . Two of the bases in nucleic acids , adenine and gua...
Imidazole in Biological Dictionary
... Purine (1) is a heterocyclic aromatic organic compound, consisting of a pyrimidine ring fused to an imidazole
ring. Purines, including substituted purines and their tautomers, are the most widely distributed kind of nitrogen-containing heterocycle in nature. ...