Addition of imidazole during binding improves purity of histidine-tagged proteins

K. Hölscher, M. Richter-Roth, and B. Felden de Neumann GPC Biotech AG, Martinsried, Germany The purity of histidine-tagged proteins* purified by metal chelate affinity chromatography can often be improved by optimizing the imidazole concentration in the ...

Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system

... JNK ), and p38 MAPK , in mammalian cells (9). Also included were wells containing both 100-µM SB203580 plus anisomycin. SB203580 is a pyridinyl imidazole MAPK inhibitor, reported to selectively inhibit p38 MAPK activity with little or no affect on JNK, ERK, or several other protein kinases (11). Plates...

New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

... convenience by providing phosphate buffer concentrates and highly pure 2 M imidazole stock solutions. This kit eliminates time-consuming manual buffer preparati...fluorescent protein (GFP-[His] 6 ; Mr 28 000) from an E. coli lysate. The imidazole concentration in the sample and binding buffers was 45 mM for His GraviTrap...

Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins

...ith either 75, 100, 250, 500, or 750 mM imidazole in the elution buffer. Following elution and a sub...kD His-tagged protein was then eluted with 5 CV of imidazole elution buffer, followed by an addition...d with the 75, 100, 250, 500, and 750 mM imidazole elution buffers were superimposed using ...

Imidazole in Biological Products

3M Imidazole Solution from BD Biosciences Pharmingen

Description: 3M Imidazole Solution 125 ml...
Company:BD Biosciences Pharmingen

Immobilized Metal affinity ProteinChip Arrays from Ciphergen

Description:...ng and subsequent affinity capture of proteins with metal binding residues. imidazole may be used in binding and washing solutions to moderate protein binding, including binding of non-specific proteins. Increasing the concentration of imidazole in the washing buffers reduces the binding of the target proteins....
Company:Ciphergen

Imidazole in Biological Definition

His-tag

... bound to it. Histidine is a good ligand for nickel so the fusion protein will bind to the column. The protein can be eluted with a solution of imidazole . Detection The his tag can also be used in detection of the protein via anti-his-tag antibodies . This can be useful in localization , ELIS...

Purine

... SMILES C1(NC=N2)=C2C=NC=N1 Purine is a heterocyclic aromatic organic compound , consisting of a pyrimidine ring fused to an imidazole ring. The general term purines also refers to substituted purines and their tautomers . Two of the bases in nucleic acids , adenine and gua...

Imidazole in Biological Dictionary

Purine

... Purine (1) is a heterocyclic aromatic organic compound, consisting of a pyrimidine ring fused to an imidazole ring. Purines, including substituted purines and their tautomers, are the most widely distributed kind of nitrogen-containing heterocycle in nature. ...