Electrotransformation of E. coli With M13 DNA
by adding the X-Gal and iptg
to the bottom agar, or by allowing the cells
... without X-Gal (in N, Ndimethylformamide; DMF) and iptg
water), then plated immediately on ... are plated with a routine amount of X-Gal and iptg
(Table 1, sample 6),
a reduction of abo...
Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C
(DHFR) gene. The plasmid was introduced into a suitable E. coli strain
and protein production was induced with iptg
Bacterial cells were harvested by centrifugation, resuspended in three
volumes of so...
Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
.... The cells were initially incubated at 37C under agitation (180 rpm) until the OD was 0.4, then at 20C. Protein expression was induced by addition of iptg
to a final concentration of 100 M.
The cells were harvested by centrifugation (3,000 x g for 15 min). The supernatant was...
Successful PCR amplification and subcloning of a GC-rich DNA fragment
...sed to transform one tube of QIAGEN EZ Competent
Cells, which were then directly plated onto LB agar plates containing
X-gal and iptg
for blue/white screening, and ampicillin at 30 g/ml.
A second set of ligation reactions were carried out, and 3 l aliquots
Mammalian and Bacterial Expression in One Vector
... (lacI). Expression is therefore inducible
in bacteria that contain the T7 RNA polymerase. ...One-half of the culture was then induced by adding iptg
to a final
concentration of 0.2 mM. The...d by Bradford assay. Equal amounts of protein from iptg
and uninduced bacterial lysates...
Production of Full-Length, Biologically Active MEK1WT and MEK1CA
...uence of MEK1 CA
was inserted at the BamH I site of the pGEX-2T plasmid. Epicurian Coli
XL1-Blue cells containing pGEX-MEK1 CA were induced by iptg
(1 mM) for
4 hours, when OD 600 reached 0.6. Cells were then collected and broken
by sonication in PBS containing 1% Triton X-100 and protease in...
pfuturbo DNA Polymerase:
A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
...uctions in the PCR-Script Amp SK(+)
cloning kit manual. The transformation mixtures were plated on LB ampicillin (50
g/ml) plates containing 0.5mM iptg
and 40 g/ml X-gal. Following overnight
incubation at 37C, recombinant (white) and nonrecombinant (blue) colonies were
counted. White colonies cont...
High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
...ate molecular weight
was detected after iptg
induction, demonstrating that Ara h 2 is only
...nt Ara h 2 expression was induced by adding 1
when the cells reached an optical densitiy of 0.6 ...ng
an overnight incubation at 22C after iptg
The final yield of soluble recombin...
X-GAL (5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactopyranoside), = 99.0% from AMRESCO, Inc.
Description:... Ultra Pure Grade. Used in conjunction with iptg
to identify bacterial colonies that contain recombinant plasmids by blue/white selection. X-Gal forms an intense blue precipitate in the presence of b...
pTriEx™-4 DNA from Novagen
Description:...om the λ p L and λl p I promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows iptg
based induction. Native protein can be expressed by cloning into the Nco I site, or native protein can be generated by cloning into the Psh AI or S...
...enotype of HT1080 human fibrosarcoma cells ... the expression of the transformed phenotype , we have isolated flat ... Cells induced with iptg
showed the transformed phenotype , independent of TGF-ß treatment. Non-induced cells did not show the transformed phenotype . ... ... parva...
... T-cell lymphotropic virus type I ... Data indicate that induction with The Inducer ™ has been shown to yield increased ... The Inducer ™ - An iptg
alternative for generating increased levels of soluble ... Inducer Motor Assemblies for most brands of gas furnaces. ... Comfortmaker 90% Furnace...