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IPTG in Biological Technology

Electrotransformation of E. coli With M13 DNA

...as circumvented by adding the X-Gal and iptg to the bottom agar, or by allowing the cells ... without X-Gal (in N, Ndimethylformamide; DMF) and iptg (in water), then plated immediately on ... are plated with a routine amount of X-Gal and iptg (Table 1, sample 6), a reduction of abo...

Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C

...ofolate-reductase (DHFR) gene. The plasmid was introduced into a suitable E. coli strain and protein production was induced with iptg (isopropyl-β-D-thiogalactopyranoside). Bacterial cells were harvested by centrifugation, resuspended in three volumes of so...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

.... The cells were initially incubated at 37C under agitation (180 rpm) until the OD was 0.4, then at 20C. Protein expression was induced by addition of iptg to a final concentration of 100 M. Pellet recovery The cells were harvested by centrifugation (3,000 x g for 15 min). The supernatant was...

Successful PCR amplification and subcloning of a GC-rich DNA fragment

...sed to transform one tube of QIAGEN EZ Competent Cells, which were then directly plated onto LB agar plates containing X-gal and iptg for blue/white screening, and ampicillin at 30 g/ml. A second set of ligation reactions were carried out, and 3 l aliquots used ...

Mammalian and Bacterial Expression in One Vector

... (lacI). Expression is therefore inducible using iptg in bacteria that contain the T7 RNA polymerase. ...One-half of the culture was then induced by adding iptg to a final concentration of 0.2 mM. The...d by Bradford assay. Equal amounts of protein from iptg induced and uninduced bacterial lysates...

Production of Full-Length, Biologically Active MEK1WT and MEK1CA

...uence of MEK1 CA was inserted at the BamH I site of the pGEX-2T plasmid. Epicurian Coli XL1-Blue cells containing pGEX-MEK1 CA were induced by iptg (1 mM) for 4 hours, when OD 600 reached 0.6. Cells were then collected and broken by sonication in PBS containing 1% Triton X-100 and protease in...

pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning

...uctions in the PCR-Script Amp SK(+) cloning kit manual. The transformation mixtures were plated on LB ampicillin (50 g/ml) plates containing 0.5mM iptg and 40 g/ml X-gal. Following overnight incubation at 37C, recombinant (white) and nonrecombinant (blue) colonies were counted. White colonies cont...

High-Level Expression of Peanut Allergens Affected by Rare Codon Usage

...ate molecular weight was detected after iptg induction, demonstrating that Ara h 2 is only ...nt Ara h 2 expression was induced by adding 1 mM iptg when the cells reached an optical densitiy of 0.6 ...ng an overnight incubation at 22C after iptg induction. The final yield of soluble recombin...
IPTG in Biological Products

X-GAL (5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactopyranoside), = 99.0% from AMRESCO, Inc.

Description:... Ultra Pure Grade. Used in conjunction with iptg to identify bacterial colonies that contain recombinant plasmids by blue/white selection. X-Gal forms an intense blue precipitate in the presence of b...
Company:AMRESCO, Inc.

pTriEx™-4 DNA from Novagen

Description:...om the λ p L and λl p I promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows iptg based induction. Native protein can be expressed by cloning into the Nco I site, or native protein can be generated by cloning into the Psh AI or S...
Company:Novagen
IPTG in Biological Dictionary

Transformed phenotype

...enotype of HT1080 human fibrosarcoma cells ... the expression of the transformed phenotype , we have isolated flat ... Cells induced with iptg showed the transformed phenotype , independent of TGF-ß treatment. Non-induced cells did not show the transformed phenotype . ... ... parva...

Inducer

... T-cell lymphotropic virus type I ... Data indicate that induction with The Inducer ™ has been shown to yield increased ... The Inducer ™ - An iptg alternative for generating increased levels of soluble ... Inducer Motor Assemblies for most brands of gas furnaces. ... Comfortmaker 90% Furnace...
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(Date:4/23/2014)... Ind. - Purdue University researchers have developed a way ... cell by using tiny gold particles with tails of ... of agricultural and biological engineering, used gold nanoparticles to ... as BRCA1 messenger RNA splice variants, which can indicate ... of these mRNA splice variants in a cell can ...
(Date:4/23/2014)... The internal surface area of the gastro-intestinal tract has ... square meters. Scientists at the Sahlgrenska Academy have used ... , "Actually, the inner surface of the gastro-intestinal tract ... says scientist Lars Fndriks. , The digestive tract, which ... through the intestines, has a length of about 5 ...
(Date:4/23/2014)... In science, "simple and accessible detection methods that ... the resolution of a single cell inside that ... chemical engineer Chang Lu. , In the Royal ... Lu has announced that he and his coworkers ... subcellular location of a protein ( Chem. Sci. ...
(Date:4/23/2014)... Fruits and vegetables are often displayed in the ... are also plenty of high-sugar foods. A new ... food is portrayed in children,s TV programmes as ... viewing, dietary habits and weight status. , Steingerdur ... food in the popular Swedish children,s TV show ...
(Date:4/23/2014)... from the Cancer Science Institute of Singapore (CSI Singapore) ... cancer specific stem cell which causes gastric cancer. This ... for the treatment of this disease and other types ... Chan Shing Leng, Research Assistant Professor at CSI Singapore, ... of a cell surface protein, CD44v8-10, marks gastric cancer ...
Breaking Medicine News(10 mins):Health News:Gold nanoparticles help target, quantify breast cancer segments in a living cell 2Health News:The surface area of the digestive tract 'only' as large as a studio apartment 2Health News:Following a protein's travel inside cells is key to improving patient monitoring, drug development 2Health News:High-calorie and low-nutrient foods in kids' TV 2Health News:Scientists identify cancer specific cell for potential treatment of gastric cancer 2Health News:Scientists identify cancer specific cell for potential treatment of gastric cancer 3
(Date:4/23/2014)... study by a University of Exeter researcher has shed ... wild camels thriving in Australia,s remote outback have become ... , Sarah Crowley, of the Environment and Sustainability Institute ... history of the camel in Australia, from their historic ... their current status as unwelcome "invader." , The deserts ...
(Date:4/23/2014)... (April 23, 2014). "I think one can argue that if ... as doing everything else that we canthen we can solve ... one of the world,s foremost climate researchers, in the current ... by SAGE. Refusing to take significant action on climate change ... needed to address the problem, Wigley explains in an exclusive ...
(Date:4/22/2014)... April 22, 2014 Scientists from the Florida campus ... protein complex that plays a critical but previously unknown ... study, which showed a novel role for a protein ... the journal eLife , a publisher supported by ... and the Wellcome Trust. , "This is a critical ...
Breaking Biology News(10 mins):How Australia got the hump with 1 million feral camels 2How Australia got the hump with 1 million feral camels 3Is nuclear power the only way to avoid geoengineering? 2Scientists identify critical new protein complex involved in learning and memory 2
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