Electrotransformation of E. coli With M13 DNA

...as circumvented by adding the X-Gal and iptg to the bottom agar, or by allowing the cells ... without X-Gal (in N, Ndimethylformamide; DMF) and iptg (in water), then plated immediately on ... are plated with a routine amount of X-Gal and iptg (Table 1, sample 6), a reduction of abo...

Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C

...ofolate-reductase (DHFR) gene. The plasmid was introduced into a suitable E. coli strain and protein production was induced with iptg (isopropyl-β-D-thiogalactopyranoside). Bacterial cells were harvested by centrifugation, resuspended in three volumes of so...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

.... The cells were initially incubated at 37C under agitation (180 rpm) until the OD was 0.4, then at 20C. Protein expression was induced by addition of iptg to a final concentration of 100 M. Pellet recovery The cells were harvested by centrifugation (3,000 x g for 15 min). The supernatant was...

Successful PCR amplification and subcloning of a GC-rich DNA fragment

...sed to transform one tube of QIAGEN EZ Competent Cells, which were then directly plated onto LB agar plates containing X-gal and iptg for blue/white screening, and ampicillin at 30 g/ml. A second set of ligation reactions were carried out, and 3 l aliquots used ...

Mammalian and Bacterial Expression in One Vector

... (lacI). Expression is therefore inducible using iptg in bacteria that contain the T7 RNA polymerase. ...One-half of the culture was then induced by adding iptg to a final concentration of 0.2 mM. The...d by Bradford assay. Equal amounts of protein from iptg induced and uninduced bacterial lysates...

Production of Full-Length, Biologically Active MEK1WT and MEK1CA

...uence of MEK1 CA was inserted at the BamH I site of the pGEX-2T plasmid. Epicurian Coli XL1-Blue cells containing pGEX-MEK1 CA were induced by iptg (1 mM) for 4 hours, when OD 600 reached 0.6. Cells were then collected and broken by sonication in PBS containing 1% Triton X-100 and protease in...

pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning

...uctions in the PCR-Script Amp SK(+) cloning kit manual. The transformation mixtures were plated on LB ampicillin (50 g/ml) plates containing 0.5mM iptg and 40 g/ml X-gal. Following overnight incubation at 37C, recombinant (white) and nonrecombinant (blue) colonies were counted. White colonies cont...

High-Level Expression of Peanut Allergens Affected by Rare Codon Usage

...ate molecular weight was detected after iptg induction, demonstrating that Ara h 2 is only ...nt Ara h 2 expression was induced by adding 1 mM iptg when the cells reached an optical densitiy of 0.6 ...ng an overnight incubation at 22C after iptg induction. The final yield of soluble recombin...

IPTG in Biological Products

X-GAL (5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactopyranoside), = 99.0% from AMRESCO, Inc.

Description:... Ultra Pure Grade. Used in conjunction with iptg to identify bacterial colonies that contain recombinant plasmids by blue/white selection. X-Gal forms an intense blue precipitate in the presence of b...
Company:AMRESCO, Inc.

pTriEx™-4 DNA from Novagen

Description:...om the λ p L and λl p I promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows iptg based induction. Native protein can be expressed by cloning into the Nco I site, or native protein can be generated by cloning into the Psh AI or S...
Company:Novagen

IPTG in Biological Dictionary

Transformed phenotype

...enotype of HT1080 human fibrosarcoma cells ... the expression of the transformed phenotype , we have isolated flat ... Cells induced with iptg showed the transformed phenotype , independent of TGF-ß treatment. Non-induced cells did not show the transformed phenotype . ... ... parva...

Inducer

... T-cell lymphotropic virus type I ... Data indicate that induction with The Inducer ™ has been shown to yield increased ... The Inducer ™ - An iptg alternative for generating increased levels of soluble ... Inducer Motor Assemblies for most brands of gas furnaces. ... Comfortmaker 90% Furnace...