IEF

Agarose IEF

Description: Agarose IEF is suitable for isoelectric focusing of large molecules and is the gel of choice for many IEF applications and immunochemical detection methods....
Company:GE Healthcare, formerly Amersham Biosciences

PROTEAN Plus Dodeca Cell, 100-120 V

Description:... The PROTEAN Plus Dodeca cell accommodates up to 12 large slab gels, matching the capacity of first-dimension runs in the PROTEAN ief system, for high-throughput 2-D applications* in a convenient format. The PROTEAN Plus Dodeca cell's cooling and recirculating capabilities, along wit...
Company:Bio-Rad Lab Equipment

PROTEAN Plus Dodeca Cell, 220-240 V

Description:... The PROTEAN Plus Dodeca cell accommodates up to 12 large slab gels, matching the capacity of first-dimension runs in the PROTEAN ief system, for high-throughput 2-D applications* in a convenient format. The PROTEAN Plus Dodeca cell's cooling and recirculating capabilities, along wit...
Company:Bio-Rad Lab Equipment

Uniphor electrophoresis units

Description:...n and Peptide Characterization: Denaturing and non denaturing SDS-PAGE gels ief in ultrathin PAGE gels (Z64,072-7, Z64,071-9& Z64,075 1) Preparative Protein and Peptide Separation: Non-denaturating PAGE gels and Agarose gels ief in Agarose Ampholyte Gels (Z64,071-9& Z64,075-1) Molecular Biology: DNA/RNA...
Company:Sigma-Aldrich

High Voltage Non-Programmable Power Supplies

Description:... From SDS-PAGE to ief to DNA sequencing, Thermo Series 90 high voltage power supplies will handle all routine electrophoretic applications. These power supplies can operat...
Company:ThermoFisher Scientific

EPS 3501 Power Supply

Description:...00 W maximum. Constant voltage, constant current, or constant power modes. Stores and recalls three protocols. Excellent for manual DNA sequencing and ief applications....
Company:GE Healthcare, formerly Amersham Biosciences

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IEF in Biological Technology

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, The University of Florida, Institute of Food and Agricultural Sciences Introduction BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated Proteins)...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, The University of Florida, Institute of Food and Agricultural Sciences Introduction BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated ...

Bruker Announces Availability of New HUPO World Congress Proteomics Posters on Web Site

...ns Poster W296 -- Comprehensive and Reliable Analysis of the Human Serum Glycoproteome Poster W298 -- High-Throughput Data Management of Shotgun ief Results by ProteinScape Poster W322 -- Hyphenated Tools for Lipidomics Poster M195 Professor Herbert Thiele, the company's Director of Bioinform...

Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A

... required, to concentrate proteins from dilute samples. Prior to ief all samples were solubilized in PSB, to which 50 mM DTT, 2 mM TB... Electrophoresis The IPG strips were focused using a PROTEAN ief cell for ~60,000 V-hr at a final focusing voltage of 10,000 V. T...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

...tly being evaluated using the PROTEAN ief cell and ReadyStripTM IPG strips to isolate and ex...by the addition of NBT/BCIP. ief and Second-Dimension Separation of Proteins ...oteins was carried out with the PROTEAN ief cell using a preset method that allowed a minimum ...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

...sopropanol/10% acetic acid. ANALYTICAL ief The isoelectric points of the two ...-Rad (IEF Ready Gel Application Guide). ief was performed on Bio-Rad ief Ready Gels (pH 58) with 20 mM NaOH as a...

Acrylamide Polymerization A Practical Approach

...r isoelectric focusing (IEF) gels. Since molecules remain in the ief gel during electrophoresis, excess ions from initiators can caus...distortion of bands. Photochemical initiation is recommended for ief gels because it is effective at low initiator concentrations. 1. ...

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IEF in Biological Products

ZOOM® IEF Fractionator from Invitrogen

Description: ...
Company:Invitrogen

ZOOM® IEF Fractionator from Invitrogen

Description: ...
Company:Invitrogen

Model 111 Mini IEF Cell, complete from Bio-Rad

Description: The Model 111 mini IEF cell is an apparatus dedicated to general analytical isoelectric focusing (IEF) using polyacrylamide or agarose slab gels. The cell includes the electrophoresis chamber and lid, graphite electrodes, casting tray, 5 glass plates, 50 sheets of gel support film for polyacrylamid...
Company:Bio-Rad

Pharmalyte 4.2-4.9 from GE Healthcare, formerly Amersham Biosciences

Description:...yte, narrow range pH 4.2-4.9, 25 ml. Category: Electrophoresis Systems & Standards & Reagents, Electrophoresis Standards and Reagents, Immobiline for ief and Carrier Ampholytes....
Company:GE Healthcare, formerly Amersham Biosciences

PhastGel DNA Silver Staining Kit from GE Healthcare, formerly Amersham Biosciences

Description:...tion sensitivity comparable to that of radioisotopes with ready-to-use silver staining kits. Category: Electrophoresis Systems & Standards & Reagents, ief & SDS-PAGE & 2-D Electrophoresis, PhastSystem with Precast Gels....
Company:GE Healthcare, formerly Amersham Biosciences

EPH electrode, cathode from GE Healthcare, formerly Amersham Biosciences

Description:... EPH electrode, cathode, 1. Category: Electrophoresis Systems & Standards & Reagents, ief & SDS-PAGE & 2-D Electrophoresis, Multiphor II Kits & Components and Accessories....
Company:GE Healthcare, formerly Amersham Biosciences

EPH electrode, anode from GE Healthcare, formerly Amersham Biosciences

Description:... EPH electrode, anode, 1. Category: Electrophoresis Systems & Standards & Reagents, ief & SDS-PAGE & 2-D Electrophoresis, Multiphor II Kits & Components and Accessories....
Company:GE Healthcare, formerly Amersham Biosciences

DryStrip aligners from GE Healthcare, formerly Amersham Biosciences

Description:... DryStrip aligners, 4. Category: Electrophoresis Systems & Standards & Reagents, ief & SDS-PAGE & 2-D Electrophoresis, Multiphor II Kits & Components and Accessories....
Company:GE Healthcare, formerly Amersham Biosciences

ReadyPrep 2-D Starter Kit from Bio-Rad

Description:... The ReadyPrep 2-D starter kit is intended for first-time users of the PROTEAN ief cell and ReadyStrip IPG strips. The kit includes a protein sample and reagents sufficient to rehydrate, focus, and transfer to second-dimension gels s...
Company:Bio-Rad

PROTEAN II xi 2-D Cell from Bio-Rad

Description:... The PROTEAN II xi 2-D system runs both gel dimensions in the same cell. Up to 16 first-dimension ief tube gels can be separated in a single run using the tube gel adaptors, which snap onto the cooling core in much the same manner as the slab gel assem...
Company:Bio-Rad

IEF in Biological Dictionary

Xanthophyll

...thophylls in the macula may indicate a protective role ... Analysis of the xanthophyll binding LHC proteins showed that the Lhcb5 ... from the ief gel showed differences in xanthophyll composition: ... ...