Successful PCR amplification and subcloning of a GC-rich DNA fragment
...sequence was successfully amplified using hotstartaq DNA Polymerase and Q-Solution. The PCR product was...of PCR primers. PCR was performed using hotstartaq DNA Polymerase and 4 other commerciall...ases. Of the 5 polymerases tested, only hotstartaq DNA Polymerase (in conjunction with Q-S...Custom and library siRNA for efficient gene silencing
...cificity and sensitivity stringent hot start with hotstartaq DNA Polymerase and increased sensitivity ... mix contains pre-optimized concentrations of hotstartaq DNA Polymerase and MgCl2, plus dNTPs and ..., specificity is significantly increased by use of hotstartaq DNA Polymerase in the master mix. This en...Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
...ithout () cleanup using the QIAquick PCR Purification Kit. PCR of the GAPDH gene was carried out for 35 cycles using the hotstartaq Master Mix Kit and primers from QIAGEN Operon. M: markers. ...QuantiTect SYBR Green RT-PCR Kit
... In one-step quantification of RNA using the QuantiTect SYBR Green RT-PCR Kit, sensitivity is enhanced by combining hotstartaq DNA Polymerase with a unique blend of Sensiscript and Omniscript Reverse Transcriptases. Sensiscript RT is designed for use with ver...Custom and library siRNA for efficient gene silencing
...development with simple master mix High specificity and sensitivity - stringent hot start with hotstartaq DNA Polymerase and increased sensitivity with the unique new multiplex PCR buffer Versatile for many applications...QIAamp DNA Blood BioRobot MDx Kit
... A 1.2 kb fragment of the single-copy Hugl gene was amplified from DNA purified with the QIAamp DNA Blood BioRobot MDx Kit, using hotstartaq DNA Polymerase in a 50 l reaction volume. No inhibition was observed using either A 1 l of eluate or B 20 ...