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Histidine in Medical News

A New Way to Predict Outbreaks: Replikin Peptide Concentration in H5N1 Influenza Virus Genome as a Marker for Lethal Outbreaks

...chemistry of rapid replication. The Doctors Bogoch called them "Replikins", and they are strictly defined by the concentration of lysine and histidine residues and the spacing between them. To demonstrate the correlation between the concentration of replikins and the lethality of influenza virus out...

AMAS Test Measures Lethal Replikin Gene Activity in Lung and Other Cancers

...ied gene has been named the Replikin Peak Gene (RPG). "Replikins(TM)" are a class of proteins containing high concentrations of amino acids lysine and histidine that have been previously determined to be related to rapid replication and virulence. Using proprietary computer algorithms constructed to identify, ...

Bacteria 'See' the Light

...se of Brucella , light activates an enzyme called histidine kinase that causes Brucella to replicate rapidly...ide, in the light." Bogomolni said that because histidine kinases are only found in bacteria -- not in human...ould do something to prevent the expression of the histidine kinase, we might impede the transition to virulenc...

Bird flu outbreak prevented by Tamiflu

...algaon and Navapur. The findings showed that the virus was sensitive to Tamiflu due the different types of amino acids like glutamic acid, asparigine, histidine and arvinone present in it. This helped in the prevention of a possible transmission of the lethal virus from birds to humans. Scientists B P...
Histidine in Medical Technology

Karmanos Researchers Study the Impact of Galectin-3 on Breast Cancer Disparity in Caucasian and Asian Women

... of galectin-3 variants in the DNA of blood, normal and cancerous tissues of Asian and Caucasian women revealed that galectin-3 is expressed as either histidine at position 64 (H64) or proline (P64) and is associated in part with increased risk for the development of breast cancer. The breakdown of the dat...
Histidine in Medical Products

Paramax-3 Rapid Test for Malaria (Pan/Pv/Pf)

Description:... For the detection of P.falciparum specific histidine rich protein-2 (Pf HRP-2), P.vivax specific pLDH and pan malaria specific pLDH, for the specific detection of P. falciparum and P.vivax malaria,...
Company:Zephyr Biomedical Systems

Parascreen® Rapid Test for Malaria (Pan/Pf)

Description:... For the detection P.falciparum specific histidine protein-2 (Pf HRP-2) and pan specific pLDH, for differentiation of P. falciparum and other malarial species viz P.vivax , P.ovale & P. malariae ...
Company:Zephyr Biomedical Systems

FalciVax Rapid Test for Malaria Pv/Pf

Description:... FalciVax is a two site sandwich immunoassay utilizing whole blood for the detection of P. falciparum specific histidine rich protein-2 (Pf. HRP-2) and P. vivax specific pLDH. The test can also be used for specific detection and differentiation of P. falciparum and P. vi...
Company:Zephyr Biomedical Systems

Paracheck Pf (P. falciparum) Rapid Test (Dipstick)

Description:... Paracheck Pf is a two site sandwich immunoassay for the detection of P. falciparum specific histidine rich protein 2 (Pf HRP-2) in whole blood samples....
Company:Orchid Biomedical Systems

Core Malaria Pf

Description:... Core Malaria Pf is a rapid self performing, qualitative, two site sandwich immunoassay for the determination of P. falciparum specific histidine rich protein 2 (Pf HRP-2) in whole blood samples...
Company:Core Diagnostics

PALUTOP+4

Features:...immunoassay using whole blood for the detection of P. falciparum specific histidine rich protein-2 (Pf HRP-2), P. vivax specific pLDH and pan malaria specific .... falciparum malaria is based on the detection of P. falciparum specific histidine rich protein 2 (Pf HRP-2) which is a water soluble protein that is released...
Company:ALL.DIAG

PALUTOP

Features:... PALUTOP™ is a two site sandwich immunoassay for the determination of P. falciparum specific histidine rich protein-2 (Pf HRP-2) in whole blood samples This test detects the presence of Pf HRP-2 in whole blood specimen and is a sensitive and specific t...
Company:ALL.DIAG
Histidine in Biological News

Natural-born divers and the molecular traces of evolution

...vers may contain 10 times more myoglobin in their muscles than terrestrial animals. The team has also found a conspicuous increase of the amino acid histidine in the myoglobin of strong divers, which may allow the animal to deal better with the accumulation of lactic acid that is frequent during long dives (...

Damon Runyon Cancer Research Foundation awards prestigious fellowships to 13 top young investigators

...PhD, with his sponsor Thomas W. Muir, PhD, at The Rockefeller University, New York, New York, is attempting to elucidate the mechanism and function of histidine phosphorylation, a particular type of protein modification that can be linked to liver cancer. He is developing novel research tools by combining syn...

Sulfurous ping-pong in the urinary tract

...modates both the donor and the acceptor of the sulfuryl group. Furthermore, during the transfer, the sulfuryl group is directly, covalently bound to a histidine side chain of ASST. Thus, the signal is first transferred from the donor to ASST and subsequently from ASST to the acceptor. Such a ping-pong mechanis...

Tick-borne encephalitis virus reveals its access code

...the endosome membrane, bringing the two membranes together. In their search for possible pH sensors, researchers have focused on five highly conserved histidine residues in the flavivirus fusion protein. The chemical properties of histidines make them prime candidatesthey switch from uncharged to having a doub...

The structure of the Mre11 protein bound to DNA

...s, Rad50 and Nbs1, can be affected. On the other hand, mutations at two histidine sites do not affect the ability of Mre11 to form dimers or interact with Ra... which affects the remodeling of DNA ends, causes fewer problems. The other histidine mutation impairs Mre11's ability to remodel both end and internal sequences...

Rice lab finds molecular clues to Wilson disease

...f the more than 1,400 amino acids in ATP7B is changed. That amino acid is a histidine located at position 1069. In the disease-causing form of the protein, this histidine is replaced with a glutamic acid. "This mutation occurs at a crucial loc...

New test could help consumers avoid surprise headaches from chocolate, wine

...iogenic amines present in the beverages in less than five minutes. The highest levels of tyramine were found in red wine, and the highest levels of histidine were found in sake, the researchers note. The beer tested contained only small amounts of these biogenic amines, they say. Some foods have more bio...

Loss of a universal tRNA feature reported

... tRNA that assists in incorporating the amino acid histidine into new proteins. histidine residues make essential contributions to protein s...VBI, have shown that tRNAs carrying the amino acid histidine in the alphaproteobacteria Sinorhizobium meliloti ...

Darwin's famous finches and Venter's marine microbes

... of niche players, but actually they are more prevalent and widespread than histidine kinases," explains Manning. Bacteria were thought to rely mostly on histidine kinases, which are structurally different from protein kinases, for all the...

Nobel Laureate finds 'elegant' explanation for DNA transcribing enzyme's high fidelity

...tacts cannot occur.? As a consequence of that alignment, to angstrom (a unit of length equal to one hundred millionth of a centimeter) precision, a histidine side chain of the trigger loop rests on the ß phosphate, the chemical constituent that must have its bond broken in order for the NTP to join the RNA ...
Histidine in Biological Technology

A New Way to Predict Outbreaks: Replikin Peptide Concentration in H5N1 Influenza Virus Genome as a Marker for Lethal Outbreaks

...olved in the chemistry of rapid replication. The Drs. Bogoch called them "Replikins," and they are strictly defined by the concentration of lysine and histidine residues and the spacing between them. To demonstrate the correlation between the concentration of replikins and the lethality of influenza virus outb...

Addition of imidazole during binding improves purity of histidine-tagged proteins

...histidine) 6 tag is one of the most common tags used to facilitate the purification of recombinant proteins. However, the presence of surface-exposed histidine residues or other complex-forming amino acids can lead to nonspecific binding of untagged host cell proteins to purification media. These untagged pro...

Tools for Proteomic Science II

...e in conjugated forms, and recognize commonly used epitope sequences, such as the influenza hemagglutinin protein (HA), human c-myc protein, six histidine residues (His6), and others. The epitope sequences can be easily inserted at the desired position within the protein amino-acid sequence using rec...

Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray Ion Trap Mass Spectrometry

...more than 5% of its total composition, but it lacks significant amounts of other oxidatively sensitive amino acids such as cysteine, histidine and tryptophan. Evidence presented recently suggests that Met residues in CaM undergo an age-dependent modification to methionine su...

Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins

...tly purified on Ni-NTA Superflow Columns using the highly selective interaction between an affinity tag of 6 consecutive histidine residues (the 6xHis tag) and nickel-nitrilotriacetic acid (Ni-NTA). The four chelating sites of NTA bind nickel ions mor...

Decontamination and inhibition of ribonucleases

... pH range, is resistant to metal chelating agents and can survive prolonged boiling or autoclaving (1). RNase A-type enzymes rely on histidine residues within the active site for catalytic activity and can be inactivated by the alkylating agent diethyl pyrocarbonate (DEPC) w...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

...t and sensitive detection of expressed proteins with anti-c-myc antibody. The HIS6 purification tag consists of six histidine residues and permits quick and easy purification of the fusion protein from bacterial cells. 5 Constitutive High-Level Mammalian Expre...
Histidine in Biological Products

adenosine 5'-O-(3-thiotriphosphate), BODIPY FL conjugated/tagged from Molecular Probes (Invitrogen)

Description:...mma-S. In addition to its potential use for binding studies the BODIPY FL ATP-Gamma-S thioether is an important new substrate for Fhit a member of the histidine triad superfamily of nucleotide-binding proteins....
Company:Molecular Probes (Invitrogen)
Histidine in Biological Definition

Affinity chromatography

... allows it to be selected for affinity binding (if the molecule is a protein these are called fusion proteins ). Such tags include six His tags (six histidine residues added to one or both ends of a protein) and GST (glutathione-S-transferase) tags. Six His tags have an affinity for nickel ions which ar...

Ames test

... strains of bacteria , generally Escherichia coli or Salmonella that already have a single mutation, for example, a strain that cannot produce histidine , an amino acid that is essential for the bacterium to grow if not provided externally with essential nutrients. Cultures of the bacteria are grown ...

Amino acid

...onine , tryptophan , valine , and (in children) histidine and arginine . General structure of an amin...ine is not optically active . H His histidine basic 155.16 7.60 1.80 9.33 6...of the nitrogen occurs, changing the properties of histidine and the polypeptide as a whole. It is used by man...

Chromatography

...affinity chromatography IMAC is a popular and powerful way to purify proteins . It is based on the specific coordinate covalent binding between histidine or other unique amino acids (either naturally present on the surface of the protein or grafted with recombinant DNA techniques ) and various immob...

Genetic code

...Proline CCA (Pro/P) Proline CCG (Pro/P) Proline CAU (His/H) histidine CAC (His/H) histidine CAA (Gln/Q) Glutamine CAG (Gln/Q) Glutamine CGU (Arg/R) Arg...

Genetic code

...Proline CCA (Pro/P) Proline CCG (Pro/P) Proline CAU (His/H) histidine CAC (His/H) histidine CAA (Gln/Q) Glutamine CAG (Gln/Q) Glutamine CGU (Arg/R) Arg...

His-tag

...cture A his-tag consists of approximately six histidine residues near the N- or C-Terminus of a Protein. The total number of histidine residues may vary. It may also be followed by a su...ther with a column that has nickel bound to it. histidine is a good ligand for nickel so the fusion protei...

Neurotransmitter

...pinephrine ( ne /noradrenaline) epinephrine ( epi /adrenaline) from tryptophan : serotonin ( 5ht /5-hydroxytryptamine) from histidine : histamine ( ? ) Polypeptides ( neuropeptides ): Bombesin/GRP: bombesin gastrin releasing peptide (GRP) Gastrins: g...

Phosphorylation

...nce tyrosine phosphorylated proteins are relatively easy to purify using antibodies , tyrosine phosphorylation sites are relatively well understood. histidine and aspartate phosphorylation occurs in prokaryotes as part of two-component signalling. Other kinds ATP , the "high-energy" exchange m...
Histidine in Biological Dictionary

Transmembrane receptor

...oy ... Keywords: Seven- transmembrane receptors , GPCR, GRK, β-arrestin, 7TMR, ... Transmembrane receptors in microorganisms, such as sensory histidine kinases and ... mechanism of transmembrane signaling by the aspartate receptor : a model. ... PolyMedix is a biotechnology company that is dev...

His tag

... A polyhistidine- tag is an amino acid motif in proteins that consists of at least six histidine ( His ) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine- tag , 6xHis- tag , and by the... ... Pathways -...

Ames test

... is a screening assay for carcinogens that uses bacteria to detect ... In the Ames test , a strain of Salmonella typhimurium auxotrophic for histidine ... Ames test summary with 5 pages of encyclopedia entries, essays, summaries, research information, and more. ... The Ames test is a proto...
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