Pathway Diagnostics Announces Patent Issuance for the Diagnostic Measurement of HIV Tropism for the Initiation or Modification of HIV Therapy
...(TM) assay is a proprietary application of molecular
hybridization based on heteroduplex
tracking technology developed by Dr.
Barbara Weiser and Dr. Harold Burger's...ry at the Wadsworth Center,
New York State Department of Health, Albany NY. heteroduplex
technology interrogates the complete HIV V3 loop, detecting sequen...
Denaturing HPLC (DHPLC)
... reversed-phase high-performance liquid chromatography (IP-RP-HPLC) technique.
It identifies mutations and polymorphisms based on detection of heteroduplex
formation between mismatched nucleotides in double-stranded PCR amplified DNA. Sequence variation creates a mixed population of heteroduplexes and h...
Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System
... programmed to denature the amplification products at 96 C for 8 minutes
and to then perform slow cooling to 40 C. Without this last step, heteroduplex
DNA may not be visible on the DGGE gels.
In the case of apoA-I, a 420 bp region at the 5' end of the gene, containing
Heteroduplex Analysis Using the DCode System
Analysis (HA) is a commonly used mutation screenin... in double-stranded DNA caused by the formation of heteroduplex
molecules have a mismatch in the double-strand, ca...
Detection of Mutations in the CYP21 Gene Using the DCode System
... C for 10 minutes to denature the PCR products and subsequently left at
room temperature for gradual renaturation, thereby generating heteroduplex
Denaturing Gradient Gel Electrophoresis
Denaturing gradient gel electrophoresis was carried out using th...
Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
Secondly, the deliberate construction of heteroduplex
molecules by pooling
homoduplex PCR products often yields unique heteroduplex
patterns, enabling iden...nute final extension
at 72 C.
DNA was formed by pairwise pooling of 10 l aliquot...
Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
...s (CDGE), 3
temperature gradient gel electrophoresis (TGGE), 4 heteroduplex
(HA), 5 protein truncation test (PTT), 6 and enzymati....25x or 1.5x
improved the resolution of the homoduplex bands and heteroduplex
thus making it easier to identify mutants from wild-type ...
Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis
... C and reannealed for 1 hour at 55 C to allow heteroduplex
Melting temperature of the 43...a positive control, and
demonstrates the heteroduplex
bands formed by polymorphic
and wild typ...specimens 4, 5, 7, 9, and 10 demonstrate
band formation, indicating an alteration in the
DGGE Analysis Using the DCode System, Rev B
...ples in lanes 13 were resolved into two
and two homoduplex bands. The heteroduplex
bands (upper bands)
migrated slower tha...ant samples. The
ability to resolve the heteroduplex
fragments and the mutant homoduplex
A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
...the differential melting characteristics of
homoduplex DNA versus heteroduplex
DNA. As heteroduplex
DNA migrates through the denaturant gradient... reduction in mobility results in a
separation of homoduplex from heteroduplex
thereby identifying a region of sequence alteration.
is a double-stranded (duplex) molecule of nucleic acid originated through the genetic recombination of single complementary strands derived from different sources, such as from different homologous chromosomes or even from different organisms. One such example is the heter...
...on of RNA. Topics ... If S1 nuclease can digest this single-strand loop and subsequently cleave the ... Moreover, S1 nuclease also cleaves heteroduplex
molecules formed from the DNAs ... S1 Nuclease Analysis. Experimental design considerations for promoter studies ... upon digestion with ...
... A heteroduplex
is a double-stranded (duplex) molecule of nucl...from different organisms. One such example is the heteroduplex
DNA strand formed in hybridization processes, usually for... Meaning of heteroduplex
DNA as a legal term. What does ...
... of reagents and reagent systems for the life ... heteroduplex
Analysis is easily applied to large ... heteroduplex
Analysis . ... heteroduplex
summary with 1 pages of encyclopedia entries, ess...