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Hepes in Biological Technology

Calcium Flux Assays using the LabChip 3000

...n 1.5 g/L sodium bicarbonate with 100 U/mL penicillin, 0.1 mg/mL streptomycin, 2 mM L-glutamine, 0.1 mg/mL Geneticin G418, 1 mM sodium pyruvate, 10 mM hepes and 10% Fetal Bovine Serum. Cells were maintained at 37 oC and 5% CO 2 and subcultured in monolayers in T75 flasks using standard cell cult...

Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

...Salt Solution (HBSS) (10X) (Invitrogen Life Technologies, cat # 14065-049), hepes 1M buffer solution (Invitrogen Life Technologies, cat # 15630-056), FBS: (F...ash buffer: this buffer contains a final concentration of 1X HBSS and 20 mM hepes diluted in sterile d.H 2 O. Important: To prevent dye extrusion by the anio...

Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor

... the microplate were treated with 0.5 M carbachol, while cells in the other half of the plate were treated with 1X Reagent Buffer (1X HBSS/20 mM hepes pH 7.4). The FLEXstation Calcium Assay Kit was used to monitor carbachol-mediated calcium mobilization (Figure 5). Z factor was calculated usi...

Gq-coupled Receptor Assays Using FLEXstation

...lution (Gibco/Invitrogen, cat# 25200-072; Tel: 1800-828-6686), Hank's balanced salt solution (HBSS) (10X) (Gibco, cat# 14065056; Tel: 1-800-828-6686), hepes 1M buffer solution (Gibco, cat#15630-080). METHODS Cell Preparation CF2Th cells were grown in D...

FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

...he FLIPR system. Growth medium was removed by aspiration and cells were washed twice with 150 l Hanks buffered saline solution supplemented with 20 mM hepes and 2.5 mM probenecid (HBSS). Dye loading buffer comprised FLUO-4-AM at 2 M final concentration, 1% fetal bovine serum and 0.1% pluronic acid. After w...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

...TM ATP Assay System, Cat. No. FF2000; Promega Corporation. 608277-2516. The kit contains Luciferase/Luciferin (L/L) reagent, ATP standard 10 -7 M in hepes buffer, pH 7.75) and ATP-free water. 3. Solid white 96-well microplates; e.g. Porvair PS white, E&G Wallac Cat. # 204003; Tel: 1-800-221-9650; ...

Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)

...0, Glutamine Pen-Strep Solution Cat. No. 9316, G418 sulfate #98529, and 1 M hepes buffer solution Cat. # 9319 were obtained from Irvine Scientific Tel. 1-800...s (2.5 M) were prepared by dilution in Hanks Balanced salt solution + 20 mM hepes (H&H) with 1 mg/ml BSA. PREPARATION OF CELLS 1....

measurement of green fluorescent proteins and a red fluorescent protein in live cells in the Analyst GT multimode reader

...Buffer (1X Hanks Balanced Salt Solution with 20 mM hepes buffer): Made from 10X Hanks Balanced Salt Solution (100 mL), 1M hepes (20 mL) and cell culture treated water (880 mL) ...Gibco/ Invitrogen Cat. #14065-056) 1M hepes (Irvine Scientific Cat. #9319) Water ...

FREE FAT CELL ASSAY

...lyse to remove ions. Dilute to 500 mL. hepes pH 7.9: 11.92 g hepes is dissolved in 50 mL water. Adjust pH to 7.9 using 1N NaOH. Dilute to 100 mL. hepes Buffer: 40 mL Krebs, 50 mL 10% HSA, 10 mL 0.5M...

Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

... cells were pre-incubated at 30C for 30 minutes in hepes [20 mM], pH 7.4, in the presence of MgCl2 [3 mM], ... filtration, followed by five washes with ice-cold hepes buffer. Bound radioactivity was determined by liqu... a desired membrane protein concentration in 25 mM hepes buffer containing 2.5 mM CaCl2 and 1 mM MgCl2, at ...
Hepes in Biological Products

Tris Hepes Buffer from NuSep Ltd

Description: Tris-Hepes kit of BG-163 & BG-165 (order number BG-166)...
Company:NuSep Ltd

0.5-10 Kb RNA Ladder from Invitrogen

Description:...the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis. 0.5-10 Kb RNA RNA Ladder is supplied at 1 g/l in 10 mM hepes (pH 7.2) 2mM EDTA. Store at -20C....
Company:Invitrogen
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