Navigation Links
Hepes in Biological Technology

Calcium Flux Assays using the LabChip 3000

...n 1.5 g/L sodium bicarbonate with 100 U/mL penicillin, 0.1 mg/mL streptomycin, 2 mM L-glutamine, 0.1 mg/mL Geneticin G418, 1 mM sodium pyruvate, 10 mM hepes and 10% Fetal Bovine Serum. Cells were maintained at 37 oC and 5% CO 2 and subcultured in monolayers in T75 flasks using standard cell cult...

Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

...Salt Solution (HBSS) (10X) (Invitrogen Life Technologies, cat # 14065-049), hepes 1M buffer solution (Invitrogen Life Technologies, cat # 15630-056), FBS: (F...ash buffer: this buffer contains a final concentration of 1X HBSS and 20 mM hepes diluted in sterile d.H 2 O. Important: To prevent dye extrusion by the anio...

Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor

... the microplate were treated with 0.5 M carbachol, while cells in the other half of the plate were treated with 1X Reagent Buffer (1X HBSS/20 mM hepes pH 7.4). The FLEXstation Calcium Assay Kit was used to monitor carbachol-mediated calcium mobilization (Figure 5). Z factor was calculated usi...

Gq-coupled Receptor Assays Using FLEXstation

...lution (Gibco/Invitrogen, cat# 25200-072; Tel: 1800-828-6686), Hank's balanced salt solution (HBSS) (10X) (Gibco, cat# 14065056; Tel: 1-800-828-6686), hepes 1M buffer solution (Gibco, cat#15630-080). METHODS Cell Preparation CF2Th cells were grown in D...

FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

...he FLIPR system. Growth medium was removed by aspiration and cells were washed twice with 150 l Hanks buffered saline solution supplemented with 20 mM hepes and 2.5 mM probenecid (HBSS). Dye loading buffer comprised FLUO-4-AM at 2 M final concentration, 1% fetal bovine serum and 0.1% pluronic acid. After w...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

...TM ATP Assay System, Cat. No. FF2000; Promega Corporation. 608277-2516. The kit contains Luciferase/Luciferin (L/L) reagent, ATP standard 10 -7 M in hepes buffer, pH 7.75) and ATP-free water. 3. Solid white 96-well microplates; e.g. Porvair PS white, E&G Wallac Cat. # 204003; Tel: 1-800-221-9650; ...

Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)

...0, Glutamine Pen-Strep Solution Cat. No. 9316, G418 sulfate #98529, and 1 M hepes buffer solution Cat. # 9319 were obtained from Irvine Scientific Tel. 1-800...s (2.5 M) were prepared by dilution in Hanks Balanced salt solution + 20 mM hepes (H&H) with 1 mg/ml BSA. PREPARATION OF CELLS 1....

measurement of green fluorescent proteins and a red fluorescent protein in live cells in the Analyst GT multimode reader

...Buffer (1X Hanks Balanced Salt Solution with 20 mM hepes buffer): Made from 10X Hanks Balanced Salt Solution (100 mL), 1M hepes (20 mL) and cell culture treated water (880 mL) ...Gibco/ Invitrogen Cat. #14065-056) 1M hepes (Irvine Scientific Cat. #9319) Water ...

FREE FAT CELL ASSAY

...lyse to remove ions. Dilute to 500 mL. hepes pH 7.9: 11.92 g hepes is dissolved in 50 mL water. Adjust pH to 7.9 using 1N NaOH. Dilute to 100 mL. hepes Buffer: 40 mL Krebs, 50 mL 10% HSA, 10 mL 0.5M...

Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

... cells were pre-incubated at 30C for 30 minutes in hepes [20 mM], pH 7.4, in the presence of MgCl2 [3 mM], ... filtration, followed by five washes with ice-cold hepes buffer. Bound radioactivity was determined by liqu... a desired membrane protein concentration in 25 mM hepes buffer containing 2.5 mM CaCl2 and 1 mM MgCl2, at ...
Hepes in Biological Products

Tris Hepes Buffer from NuSep Ltd

Description: Tris-Hepes kit of BG-163 & BG-165 (order number BG-166)...
Company:NuSep Ltd

0.5-10 Kb RNA Ladder from Invitrogen

Description:...the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis. 0.5-10 Kb RNA RNA Ladder is supplied at 1 g/l in 10 mM hepes (pH 7.2) 2mM EDTA. Store at -20C....
Company:Invitrogen
Other Tags
(Date:3/26/2015)... 2015 Behavioral Research Group, LLC ... the release of BathroomMap® , a new bathroom ... to public restrooms for travelers, especially those with medical ... does not facilitate a paid, social or crowdsourced model, ... rate local establishments that have clean, available restrooms in ...
(Date:3/26/2015)... 26, 2015 “The SWEET Act is an ... options more available and help parents who are working to ... a reasonable approach to support public health, and we will ... for the health of all families,” said Dr. Jane Delgado ... on the introduction of the Sugar-Sweetened Beverage Tax (SWEET) Act ...
(Date:3/26/2015)... 26, 2015 Visiting Nurse Association ... 18th annual gala fundraiser, Lighting the Way, Brightening the ... at The Madison Hotel in Morristown, NJ. , ... by renowned musician Rio Clemente, delightful cuisine, an extensive ... Dave Hertzel. Additionally, two prestigious awards will be presented ...
(Date:3/26/2015)... Saving Moses is a global humanitarian organization ... but least available. As one of their staple programs, ... the Benguela province of Angola to treat babies and ... to their clinics, staff employed the art of photography ... in a nation the The New York Times recently ...
(Date:3/26/2015)... U.S. medical real estate sales ... according to an exclusive new report now available ... estate (HRE) data firm also announced that it ... Gold-Level Partners: Harrison Street Real Estate Capital and ... still going strong, yields compressed, prices per square ...
Breaking Medicine News(10 mins):Health News:New Bathroom Finder App, BathroomMap®, Helps People with Medical Needs Who Urgently Need a Bathroom When Traveling 2Health News:New Bathroom Finder App, BathroomMap®, Helps People with Medical Needs Who Urgently Need a Bathroom When Traveling 3Health News:National Alliance for Hispanic Health Statement on Introduction of SWEET Act in 114th Congress 2Health News:Morristown VNA Fundraiser to Honor Distinguished Community Members 2Health News:Saving Moses Utilized Photography in an Effort to Engage Mothers and Their Babies on a Recent Visit to Angola 2Health News:Medical Real Estate Sales Topped $11.3 Billion in 2014 2Health News:Medical Real Estate Sales Topped $11.3 Billion in 2014 3Health News:Medical Real Estate Sales Topped $11.3 Billion in 2014 4
(Date:3/26/2015)... The Granite Club, Canada,s ... announced it has implemented a state-of-the-art access and security ... and staff, while restricting access to authorized users only. ... IMID Access system because it has the perfect combination ... in addition to unparalleled security," said James DiRenzo ...
(Date:3/24/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/hdgz7h/global_iris ... Iris Recognition Market 2015-2019" report to their offering. ... market to grow at a CAGR of 23.5 percent ... the present scenario and the growth prospects of the ... calculate the market size, the report considers the revenue ...
(Date:3/24/2015)... ( http://www.researchandmarkets.com/research/kvdtcw/face_and_voice ) has announced the addition of the ... report to their offering. This report analyzes the worldwide ... following Segments: Face Biometrics, and Voice Biometrics. The report provides separate ... Japan , Europe , ... Africa , and Latin America . ...
Breaking Biology News(10 mins):Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2Global Iris Recognition Market 2015-2019 with 3M Cogent, Bioenable Technologies, Cross Match Technology, Iris ID Systems & Safran Dominating 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6
Other Contents