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HeLa in Medical News

Carnegie Mellon technique accelerates biological image analysis

...ing text analysis, Web analysis and medical diagnosis. For this paper, the researchers applied their techniques to analysis of protein patterns within hela cells. They found the technique speeded analysis by several orders of magnitude. In high-throughput screening processes used for drug discovery and ...

Absorption Of Carbon Nanotube Measured In Worms Revolutionize Cancer Research

...ctive isotope, which enabled the nanotubes to be tracked and quantified, as they were absorbed into living cells. Researchers used cancer cells called hela cells, and also measured nanotube uptake in an earthworm and an aquatic type of worm. The findings were presented Sunday at the 231st American Ch...
HeLa in Medical Technology

St. Jude Study Finds Treatment With New Drug Might Make Tumor Cells More Sensitive to Therapy

...of these events. This occurred not only in standard laboratory cells called hela cells, but also in human breast cancer cells. Overall, the response to CP46...dy this effect in those models. Finally, the team showed that exposure of hela cells to CP466722 for only four hours inhibits ATM sufficiently to enhance ...
HeLa in Medical Dictionary

Gene

... microarray gene expression profiling and data-analysis algorithms ... We also compared gene expression patterns in astrocytes with those in human hela ... To identify functionally related gene sets that are differentially perturbed in ... This procedure generates an enrichment score (ES) for ea...
HeLa in Biological News

Novel structure proteins could play a role in apoptosis

... Cell Cycle (Vol. 7, Iss. 12), the Temple researchers examined the gene in hela cells, or cervical cancer cell lines, to further characterize the possible ...leading the researchers to conclude that at least in this model of in-vitro hela cells, NSP 5a3a, working in association with B23, may have a role in apopto...

Cancer drug delivery research at Case Western Reserve University cuts time from days to hours

...peed of drug administration in mice is due in part to the much more efficient dispersion of the NP delivered drug. When tested on human cells called hela a line of laboratory-grown human cells used in testing most of the drug is uploaded within one hour. Testing on human beings may not begin for so...

Carnegie Mellon scientists develop nanogels that enable controlled delivery of carbohydrate drugs

...d that its novel nanogels could be used to encapsulate doxorubicin, an anticancer drug. When the scientists mixed the doxorubicin-loaded nanogels with hela cancer cells in the laboratory, the doxorubicin was released, penetrating the cancer cells and significantly inhibiting their growth. They carried out...

Carbon nanotube absorption measured in worms, cancer cells

...active isotope, which enabled the nanotubes to be tracked and quantified as they were absorbed into living cells. Researchers used cancer cells called hela cells, and also measured nanotube uptake in an earthworm and an aquatic type of worm. The findings were presented Sunday at the 231st American Che...
HeLa in Biological Technology

Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators

Stable reporter cell lines for signal transduction pathway readout Lisa Hexdall Li Xu Alan Greener Mary Buchanan John Bauer Chao-Feng Zheng Stratagene Stratagene now offers the PathDetect HLR-CREB, HLR-Elk1, and HLR-cJun, cell lines F ...

The New Affymetrix GeneChip Scanner 3000

...gets containing the 53 transcripts presumed to be Absent (0.00 pM) based on historical results from GeneChip HG-U133 expression assay analysis of this hela cell line, and then reconstituted to 0.75 pM, 1.50 pM, or 3.00 pM in the final Figure 3. Comparison of Average Percent False Change Between GeneChip S...

Measuring siRNA-mediated knockdown of IL-8 mRNA

...eter were used to measure IL-8 mRNA in PMA-induced hela cells, following siRNA treatment. Quantitative ass...iated knockdown of IL-8 gene expression in induced hela cells is employed as a model system. Qua... Induction of IL-8 gene expression by PMA/LPS in hela cells Approximately 5,000...

Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ

...s spectrometer, GE Healthcare, 110027-38, Edition AA (2005). 3. Beausoleil, S. A. et al . Large-scale characterization of hela cell nuclear phosphoproteins. Proc. Natl. Acad. Sci. USA 101 , 12130–12135 (2004).

b...

2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ

...r ion trap mass spectrometer, GE Healthcare, 11-0027-38, Edition AA (2005).

3. Beausoleil, S. A. et al . Large-scale characterization of hela cell nuclear phosphoproteins. Proc. Natl. Acad. Sci. USA 101 , 12130–12135 (2004).

4. Jiang, S. et al . Scaffold attachment fac...

Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system

...>* An MOI of 10 infectious units (ifu) per cell in hela cells has been calculated as optimum for the EGFP-... > Results Optimization of MOI in hela cells To ensure good assay performance,...rus into cells. Figure 1 shows data for transduced hela cells treated with drugs at a range of MOI. The da...

Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system

...duct.

* An MOI of 50 to 60 ifu/cell in hela cells has been calculated as optimum for the EGFP-...owed calculation of the transduction efficiency in hela cells at three MOI loadings (Table 1). hela cells were set up at 1 × 10 6 cells/well of...

Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment

...ed by localization of -galactosidase activity in hela cell monolayers and murine epidermal cells and by the detection of hela cells positive for the green fluorescent protein... CELL CULTURE AND ANIMALS hela cells were cultured in minimal essential medium (M...

Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A

...te Preparation Lysates of HEK 293 and hela cells were prepared according to two techniques. ... a dose response observed with ELISA of hela lysates, even at the highest protein concentration...th sandwich antibody methods detected total ERK in hela lysates with protein concentration rang...

Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A

... gel electrophoresis (PAGE) of the synthesome from hela cells revealed the presence of several ... CELL CULTURE Suspension cultures of hela cells were grown in Jokliks modified Eagles ...Baril, E. F., A 21S enzyme complex from hela cells that functions in simian virus 40...
HeLa in Biological Products

Mouse Anti-Human Clusterin alpha chain Monoclonal Antibody, Unconjugated, Clone 41D from Upstate

Description:...from human serum Formulation: 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide Quality Assurance: routinely evaluated on RIPA lysates from hela cells or MCF-7 cell lysates. Recognizes clusterin α chain...
Company:Upstate

Rat Anti-Mouse ABCA1 Monoclonal Antibody, Unconjugated, Clone 5A1-1422.11 from ABR-Affinity BioReagents

Description:... ABCA1 Immunogen: Mouse ABCA1-expressing hela stable transfectants. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Company:ABR-Affinity BioReagents

Rabbit Anti-Human Connexin 36 Monoclonal Antibody,, Clone ZMD.257 from Invitrogen

Description:...onnexin 36 antibody Cat. No. 51-6300. Specificity: Reacts with mouse Connexin 36. Reactivity: Mouse (positive control: mouse Connexin 36-transfected hela cell lysates mouse brain retina and pancreas homogenates). Based on sequence homology reactivity with Human rat and zebrafish. Applications: Western ...
Company:Invitrogen

Rabbit Anti-PRK2 Polyclonal Antibody, Unconjugated from Upstate

Description:... (DKKVKPPFIPTIRGRE) of human PRK2. Purification immunoaffinity purified Quality Assurance Routinely evaluated by immunoblot in RIPA lysates from hela cells. Stability 1 year at -20°C from date of shipment...
Company:Upstate

Rabbit Anti-HMGN2 / HMG-17 Polyclonal Antibody, Unconjugated from Upstate

Description:... 0.05% sodium azide before the addition of glycerol to 30% Quality Assurance: routinely evaluated by immunoblot on acid extracts of murine 10T 1/2 and hela cells Recognizes HMGN2/HMG-17; slight cross-reactivity with HMGN1/HMG-14...
Company:Upstate

Anti-MPM-2, phospho-Ser / Thr-Pro (Mitotic protein monoclonal #2) Antibody, Agarose Conjugated from Upstate

Description:...mulation: PBS with 0.05% sodium azide Quality Assurance: routinely evaluated by immunoprecipitating mitotic MPM2 antigens from colcemid treated human hela cells as shown by subsequent immunoblot using the monoclonal anti-phospho-Ser/Thr-Pro, MPM2 (05-368)...
Company:Upstate

Rabbit Anti-Mi-2 Polyclonal Antibody, Unconjugated from Upstate

Description:...mulation: 0.7M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide, containing 30% glycerol Quality Assurance: routinely evaluated by immunoblot on hela nuclear extract Recognizes Mi2...
Company:Upstate

Rabbit Anti-MAPKAPK-2, phospho (Thr222) Polyclonal Antibody, Unconjugated from Calbiochem

Description:...0% glycerol, pH 7.5. Recognizes the ~47 kDa MAP kinase-activated protein kinase 2 (MAPKAPK-2) protein phosphorylated at Thr 222 in anisomycin-treated hela cells. ...
Company:Calbiochem

Mouse Anti-AP-2 alpha Monoclonal Antibody, Unconjugated, Clone 8G8/5 from Upstate

Description:...1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% Quality Assurance: routinely evaluated by immunoblot on hela nuclear extract Recognizes AP-2α...
Company:Upstate

Ubiquitin Fraction I Extract from GeneTex

Description:...ngelmans syndrome, and Liddle syndrome. GTX80876 is the protein fraction of hela cell extract that does not bind to anion exchange resin. GTX80876 contains ...tivity. Type: Proteins and peptides Form: 8 mg/ml protein from hela cells in 25 mM HEPES, pH 7.6. ...
Company:GeneTex
HeLa in Biological Definition

HeLa

Hela is also the German name for Hel, Poland and the cruiser SMS Hela In biological and medical research, a HeLa cell is a cell which is derived from cervical cancer cells taken from a woman named Henrietta Lacks , who died from the cancer in 1951 , and circulated (without La...
HeLa in Biological Dictionary

Tubulin

...Da subunits, designated ... rate and extent of polymerisation into microtubules, tubulin requires GTP. ... Confocal immunofluorescent analysis of hela cells, using β- Tubulin (9F3) Rabbit mAb (green) ... 2148 α/β- Tubulin Antibody ... 1032 β- Tubulin Blocking Peptide ... Western blot analysis o...

MSH2

...0 µg. Human. Recombinant, E. coli. Pricing Info ... Biomolecule Products : MSH2 (clear filter) Type : Proteins (clear filter) ... ... analysis of hela cells using MSH2 (D24B5) XP™ Rabbit mAb (green) ... MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential ... Feedb...

Hela cell

... A hela cell is an immortal cell line used in scienti...tionary and glossary with medical definitions ... hela cell : One of the cells grown from the cervical cancer of a young African ... What is hela cells ? Meaning of ...

Genomic DNA

... Request Info ... Human DNA (cytosine-5) Methyltransferase (Dnmt3A) Amino-terminal Ab ... 5-Aza-dc Treated Jurkat Genomic DNA . CpG Methylated hela Genomic DNA ... ...

G2 phase

...ree Online Dictionary and Thesaurus. G2 phase -specific proteins of hela cells. A A Al-Bader, A Orengo, and P N Rao ... The S phase cells differ.... botulinum C2 toxin delays the G2 /M phase transition in synchronized hela cells. ... not allow one to distinguish between cells in G2 phase and...

Base sequence analysis

...sequence analysis revealed that the amino acid sequence of ... Base Sequence . Cloning, Molecular* DNA/ analysis . Endonucleases/metabolism. hela Cells/enzymology ... Analysis . The GA generates images of each base of each cluster. ... Base Calling: cluster intensities are used to output...

Band shift assay

...nd shift assay with RNA probes. Jochen jochen at iigb.na.cnr.it ... I'm trying to get a band shift with cytoplasmic and nuclear extracts from hela ... Definition of band shift assay ... Gel Shift , or Band Shift Assay , or Electrophoretic Mobility Shift Assay (EMSA) ... s. The ...
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