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Hairpin in Medical News

The vulnerable cancer cell

...as informed their decision to focus on using short hairpin RNAs (shRNAs) -- single strands of RNA that fold b...lledge's team generated about 75,000 bits of short hairpin RNAs that can be inserted into retroviruses. When ...rcoding" method to track the diverse pool of short hairpin RNAs in parallel. In the barcoding method, every s...

UCSF creates fast, affordable tool for finding gene 'on-off' switches

... UCSF scientists have created a method of quickly identifying large numbers of the genetic material known as short hairpin RNA also called shRNA that turns genes on and off. The method, which the research team has used to create a library of 22,000 of these "on-off" ...

Foot Injuries Plague Athletes During Fall Sports Season

...sociation] (APMA), both professional and amateur athletes are at an extremely high risk for injuring their lower limbs during play. Whether performing hairpin turns on artificial turf or taking part in a powerful, high-speed collision with other players, foot and ankle injury risks for athletes run rampant -...

Tufts researchers discover link between DNA palindromes and disease

... yak, a yam, a hat, a canal-Panama!'" Past DNA research had shown that long palindromes change the shape of the molecule from a double helix into a hairpin or cruciform like structure in a test tube. It was not known, however, whether these changes can occur inside cells and, if so, affect DNA functioning...

The RNA drug revolution -- a new approach to gene therapy

...ere to stay. The excitement surrounding RNAi and the two main approaches to delivering RNA-based therapeuticsas mature siRNA molecules or as short hairpin RNAs (shRNAs)relates to the discovery of native microRNA molecules in human cells and their intrinsic ability to block the expression of a target gene...
Hairpin in Medical Technology

Cequent tkRNAi Technology to be Presented at the Centennial Meeting of the American Association for Cancer Research

...will be held April 18 - 22, 2009, at the Colorado Convention Center in Denver, Colorado. Sunday, April 19 Characterization of a Multiple hairpin Expression Construct for TransKingdom RNA Interference (tkRNAi) 8 am - noon Hall B-F Cequent's Group Leader for...

Cequent to Present First Proof of Activity of an Oral RNAi Drug in Non-Human Primates at February Keystone Conference

...thologic abnormalities noted. Orally administered bacteria expressing short hairpin RNA (shRNA) against beta-catenin significantly reduced messenger RNA (mRNA)...d shRNA in the intestinal mucosa, proving successful delivery of the active hairpin RNA component by the bacterial system. Cequent developed the current tk RN...

Cequent Pharmaceuticals' Johannes Fruehauf, MD, Ph.D., to be Featured Speaker at RNAi World Congress in Boston - May 2, 2008

...diseased tissues. In a proprietary process, we modify live, nonpathogenic E. coli bacteria to produce and deposit mediators of RNA interference (short hairpin RNA) directly into the target cells. The nonpathogenic bacteria are subsequently degraded by the host cells without adverse effects, and eliminated by...

CytRx Announces Positive Results of Arimoclomol in Preclinical Stroke Recovery Studies

...nventor of fundamental technology for stabilizing RNAi and of RNAi nanotransporters, Greg Hannon, Ph.D., discoverer of RNAi mechanism (RISC) and short hairpin RNAi (shRNAi), and Michael Czech, Ph.D., a leader in the application of RNAi to diabetes and obesity. RXi's CEO, Tod Woolf, Ph.D., previously co-inven...

Cequent Pharmaceuticals Founder to Present tkRNAi Technology at the American Association of Cancer Research Annual Meeting in Los Angeles

...egant "Trojan horse" solution to the delivery problem; nonpathogenic bacteria are modified to produce and deposit mediators of RNA interference (short hairpin RNA) directly into the target cells. This method, which has been shown to induce gene silencing and therapeutic effects in multiple model systems, inc...

CytRx Announces RNAi Delivery Nanoparticle Technology Exclusively Licensed to Subsidiary, RXi Pharmaceuticals, Described in Peer-Reviewed Journal

...nventor of fundamental technology for stabilizing RNAi and of RNAi nanotransporters, Greg Hannon, Ph.D., discoverer of RNAi mechanism (RISC) and short hairpin RNAi (shRNAi), and Michael Czech, Ph.D., a leader in the application of RNAi to diabetes and obesity. RXi's CEO, Tod Woolf, Ph.D., previously co-inven...

CytRx Announces Clinical Results from Its Rising Multiple Dose Trial with Arimoclomol for ALS

...nventor of fundamental technology for stabilizing RNAi and of RNAi nanotransporters; Greg Hannon, Ph.D., discoverer of RNAi mechanism (RISC) and short hairpin RNAi (shRNAi); and Michael Czech, Ph.D., a leader in the application of RNAi to diabetes and obesity. RXi's CEO, Tod Woolf, Ph.D., previously co-inven...

CytRx Reports Promising Data from Its Open-Label Extension Clinical Trial of Arimoclomol in ALS

...nventor of fundamental technology for stabilizing RNAi and of RNAi nanotransporters; Greg Hannon, Ph.D., discoverer of RNAi mechanism (RISC) and short hairpin RNAi (shRNAi); and Michael Czech, Ph.D., a leader in the application of RNAi to diabetes and obesity. RXi's CEO, Tod Woolf, Ph.D., previously co-inven...
Hairpin in Biological News

Researchers gain insight into mechanism underlying Huntington's

...and myotonic dystrophy, respectively tend to form hairpin structures via strand slippage in the newly synthe...sociated with DNA replication and/or repair. These hairpin structures are highly thermo-stable and do not "me...fied a novel DNA repair pathway referred to as DNA hairpin repair (HPR), which specifically targets TNR...

Researchers observe single protein dimers wavering between two symmetrically opposed structures

...ced within the bacteria. This small "homodimer" is made up of two identical hairpin coils of amino acids, and has often been used as a model system to study pr...: the "anti" wild type (natural) conformation occurs when the "head" of one hairpin is paired with the "tail" of the second, and the "syn" structure is made up...

Properties of unusual virus revealed in research

...A is shaped like a hairpin, whereas the E. coli bacterium's DNA is not shaped like a hairpin. Once the N4 RNA polymerase interacts with the phage's hairpin DNA, it begins to change its shape from a fisted form to a cupped form. By opening up, the RNA polymerase exposes its active site, which allows it to...

Researchers study virus with unusual properties

...A is shaped like a hairpin, whereas the E. coli bacterium's DNA is not shaped like a hairpin. Once the N4 RNA polymerase interacts with the phage's hairpin DNA, it begins to change its shape from a fisted form to a cupped form. By opening up, the RNA polymerase exposes its active site, which allows it to...

MGH researcher Gary Ruvkun named a co-recipient of the Lasker Award

...ad been snipped out of a larger RNA molecule that folds back on itself in a hairpin shape. Later that year Ruvkun published evidence that animals from fish to...hat the microRNAs of both lin-4 and let-7 are released from their precursor hairpin RNA molecules by the enzyme Dicer, which is also critical to the RNA interf...

UD researchers discover novel 'gene toggles' in world's top food crop

...e sense and the antisense to a small region that includes the microRNA. In addition, splicing of the precursor of natural antisense microRNAs allows a hairpin to form, and hairpins are a requirement for any microRNA to be made. Green noted that such microRNAs are not present in the common research plant A...

Stanford researchers make first direct observation of 3-D molecule folding in real time

...tly, tugging on its own incipient long tail and changing its shape in a way that will affect its eventual protein product. That RNA tail usually has a hairpin fold that straightens out when pulled. By tugging out this kink in the RNA, a riboswitch changes how the RNA is translated into protein, effectively t...

MIT reports new twist in microRNA biology

...mbryo. These microRNAs fold into relatively simple hairpin structures, with two stretches of near-perfect com... back onto each other. One of the two 'arms' of a hairpin is then processed into a mature microRNA. The ...ellis reported that both arms of a single microRNA hairpin can also produce distinct, functional microRNAs, w...

When it comes to gene transcription, random pauses aren’t quite so random, study finds

...tes, they behaved as predicted, pausing briefly at all of the backtrack and hairpin pause sites, but not actually backtracking or forming hairpins. "That's...ome researchers have argued that all pauses might be associated with either hairpin formation or backtracking, but the Cell study contradicts that assumption. ...

Virus uses tiny RNA to evade the immune system

...recursors. MicroRNAs are made from messenger RNA molecules with distinctive hairpin folds. The hairpin structure is diced into a microRNA segment that works with another complex ...
Hairpin in Biological Technology

Nastech Assigns Intellectual Property Related to the Development of RNA-Based Therapeutics to its Subsidiary, MDRNA, Inc.

...NAi-Based Therapeutics for Allergic Rhinitis and Asthma -- 20050008617 Compositions and Methods for Delivery of Short Interfering RNA and Short hairpin RNA -- WO2004028471 Influenza Therapeutic -- 20040242518 Influenza Therapeutic Additionally, Nastech has exclusive and non-exclusive rights under...

Team demos safety of RNA therapy

...arge doses of one type of RNA used for RNAi, short hairpin RNA, disrupted another key RNA pathway, the microR...rson. That first paper demonstrated that short hairpin RNA could lead to mouse fatality, he said. Researc... retroviruses to deliver genes that code for short hairpin RNA, which is a precursor to siRNA. Once the gene ...

Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

...ped by Kramerand associates to detect specific nucleic acids in homogeneous solutions. 1 4 They are single-stranded oligonucleotides that exist in a hairpin shape because of thecomplementary arms at the ends which bind together to form a stem. Thestrained loop portion contains the nucleotide that is comple...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

...her DABCYL linked to the 3' end. The melting temperatures of the hairpin stems and probe sequences were 6366C. To estimate the stability of the molecular beacons hairpin stem structure, the DNA folding program m-fold (available at htt...

RNA Interference and Gene Silencing: History and Overview

...hese vectors have been engineered to express small hairpin RNAs (shRNAs), which get processed in viv...annon GJ, and Conklin DS. (2002). Short hairpin RNAs (shRNAs) induce sequence-specific ...erence by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. A...

Superior Gene Silencing Using Adenoviral Vectors

... or mutant polyglutamine (polyQ) alleles (Atx-Q166-GFP) containing a CAG expansion in Ataxin-3 (2). Adenoviral vectors that expressed hairpin siRNAs targeting either the wild type or the mutant allele were designed generated from these plasmids and used to infect Cos-7 ...

siRNA Expression Vectors with Selectable Markers

...promoter to drive expression of a short hairpin RNA that mimics the structure of an siRNA (See Wh... sidebar). The insert that encodes this hairpin is designed to have two inverted repeats separated...the vector constitutively expresses the hairpin RNA, which induces silencing of the tar...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

...f two genes (GAPDH and cyclophilin) were selected and prepared using the Silencer siRNA Construction Kit. Sequences encoding hairpin siRNAs to the same target sequences were sub-cloned into p Silencer 2.0-U6 and 3.0-H1. The siRNAs and siRNA expression vectors ...

RNAi: Get the Whole Story

...om an siRNA experiment in which cells were transfected with either a chemically synthesized siRNA or with a plasmid expressing a hairpin siRNA--both targeting human GAPDH. The PARIS Kit was used to isolate RNA and protein from either total cell lysates, or from the...

RNAi: A How To for New Users

...s In mammalian cultured cells, RNAi is typically induced by siRNA introduced directly or expressed as a hairpin structure from a DNA construct within the cells. Currently, there are six methods for generating siRNAs: ...
Hairpin in Biological Products

Amplifluor UniPrimer II Fluorescein from CHEMICON

Description:... The Amplifluor UniPrimer-fluorescein is a hairpin primer labeled with a fluorescent energy transfer pair (fluorescein and non-fluorescent quencher) and a 3 single-stranded oligonucleotide tail. The U...
Company:CHEMICON

pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation

Description:...iginate from different transcripts expressed in the same cell or both siRNA strands may be part of a single transcript that folds back to form a short hairpin structure. The expression cassette(s) encoding the siRNA strands can be delivered to cells in the same fragment of DNA (hairpin expression cassette) o...
Company:Mirus Bio Corporation

pMIR-mU6, Mouse U6Template (Vector) from Mirus Bio Corporation

Description:...iginate from different transcripts expressed in the same cell or both siRNA strands may be part of a single transcript that folds back to form a short hairpin structure [2] (Figure 1). The expression cassette(s) encoding the siRNA strands can be delivered to cells in the same fragment of DNA (hairpin express...
Company:Mirus Bio Corporation

pMIR-mU6-Luc, Mouse U6 Luciferase Control Vector from Mirus Bio Corporation

Description:...iginate from different transcripts expressed in the same cell or both siRNA strands may be part of a single transcript that folds back to form a short hairpin structure. The expression cassette(s) encoding the siRNA strands can be delivered to cells in the same fragment of DNA (hairpin expression cassette) o...
Company:Mirus Bio Corporation
Hairpin in Biological Definition

Beta sheet

...e alternate side of the sheet are polar or charged (hydrophilic). Some sequences involved in a β sheet, when traced along the backbone, take a hairpin turn in orientation (direction), sometimes through one or more prolines . See also : tertiary structure -- α helix -- collagen hel...

RNA

...other part of the same molecule (for example, 5' -ACUCGA- 3' and 5'-UCGAGU-3'), so that the two strands bind together. This allows the formation of hairpin loops , coils, etc., which then direct the formation of higher-order structures. Synthesis RNA is made by an enzyme , RNA polymerase , usin...
Hairpin in Biological Dictionary

Hairpin

The type of hair pin (bobby pin) from which a ' hairpin turn' takes its name. ... Alpe d'Huez in the French Alps, famous for its 21 hairpin bends ... Stem-loop intramolecular base pairing is a pattern that can occur in single-stranded DNA or, more commonly, in RNA. The st...

Small nuclear RNA

...snRNA) ... Small Nucleolar RNA (snoRNA) ... BioInfoBank Library :: RNA , Small Nuclear :: biosynthesis :: RNA interference by small hairpin RNAs synthesised under control of the human 7S K RNA promoter. TFIID ... BioInfoBank Library :: RNA , Small Nuclear :: genetics :: Stru...

S1 nuclease

...S1 Nuclease from Aspergillus , Methods in ... S1 NUCLEASE DIGESTIONS. S1 nuclease removes 5' and 3' overhanging single-stranded DNA and hairpin loops. ... Be careful with S1 nuclease . ... Nuclease Protection Assays (NPAs): The Basics covers the use of NPAs, including both RPAs and S...

RNA interference

...most current information on RNA Interference . Promega provides systems for dna-directed RNA interference that enable expression of short hairpin RNAs in vivo. ... The RNA Interference chapter in our ... RNA interference : A Nature Reviews journals web focus on RNAi - a selection of...

Ribozyme

...e Rock / Metal / Alternative music singles, watch music videos, listen to free streaming mp3s, & read Ribozyme's ... Crystal structure of a hairpin ribozyme -inhibitor complex with implications for ... and siRNA-mediated gene silencing in vivo with ribozyme -based reporters. ... Biotechn...

Loop of Henle

...mal convoluted tubule to the distal convoluted tubule. The loop has a hairpin bend in the renal medulla. The main function of this structure is to c...llecting duct ... A loop of Henle descends into the medulla, makes a hairpin turn, and returns to the cortex. ... In the Loop of Henle there i...

Labium

...a, 1979b) ... Male and female mosquitoes have different feeding habits and techniques. ... As it goes into the skin the labium folds back like a hairpin and the mosquito ... ... scrotum and penis (or labium majus and clitoris) ... lower abdominal wall and anterior scrotum/ labium majus ... of ...

Inverted Repeats

...se currently include, the Inverted Repeats Finder algorithm, query and ... The consequence of these inverted repeats is that they can form hairpin or ... However inverted repeats are also one of the sources of genome instability and ... Inverted , Repeats ,biological ,biology dictionar...
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