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HUVEC in Biological Technology

Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System

...reted IL-6 and IL-8 in the tissue culture media of huvec treated with TNF- or IL-1. Because no wash steps ...thermore, the cell-surface expression of ICAM-1 on huvec can be simultaneously quantified by a fluorescent ...ction antibodies (Figures 1 and 2). Alternatively, huvec cells are detected by the addition of CentriRed St...

Got Small RNA?

...d both at the mRNA and protein levels in these cells, but not in huvec cells electroporated with a control huvec cells (1.5x10 6 ) were electroporated with 10 g of an siRNA targ...

Delivering siRNAs to Difficult Cell Types

...types require different electroporation parameters. For example, in a standard 1 mm electroporation cuvette, huvec cells require 1 electropulse, 150 S, and 250 Volts; whereas primary NHDF-Neo cells require 2 pulses, 70 S, a...

Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection

...eal-time RT-PCR. In huvec cells, use of (function() { var po = document.createElement('script'); po.type = 'text/javascript'; po.async = true; po.src = ''; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(po, s); })();