Any exposure to tobacco smoke during pregnancy is risky
...nd sometimes even direct exposure through maternal smoking ?or produced contradictory results.
The original studies looked at mutation rates at the hprt
gene located on the X chromosome in cord blood samples from newborns. Dr. Grant's analysis pooled the studies' data, looking for frequency of induced ...
Protect RNA and Improve Target Cell Identification
...d to isolate RNA from
the LCM sample shown in Figure 2. RNA from hprt
was amplified from this
sample by qRT-PCR (Figure 5A). In additi... Samples.
(A) One-step qRT-PCR reaction to amplify
mRNA was performed with total RNA isolated from human ovary LCM sample
Housekeeping Genes: Universal Positive
Controls in siRNA Knockdown Experiments
The housekeeping genes G6PDH and hprt
can be used
as a positive control for gene knockdown experiments
when siRNA transfection conditions have to be optimized.
The LightCycler Instru...
Quantification of siRNA Silencing Efficiency
Using the LightCycler System
...ata from the housekeeping
genes were used to normalize the GFP results (Figure 3).
Of the five tested housekeeping genes (β2M, G6PDH,
and PBGD) the expression level of
β2Microglobulin (β2M) was closest to the expression
level of GFP (Figure 3a). Therefore it was selecte...
High-Throughput Isolation of Total RNA
...cells was also subjected to molecular
beacon RT-PCR analysis 5 using primers and a beacon specific
for the low-abundance target hprt
4 ). The beacon hybridizes quantitatively to the target sequence and
emits fluorescence in this conformation. The thres...
...ltransferase salvages adenine, while hypoxanthine-guanine phosphoribosyltransferase (HPRT) salvages guanine and hypoxanthine. Genetic deficiency of hprt
causes Lesch-Nyhan syndrome .
Purine was named by the German chemist Emil Fischer in 1884 . He synthesized it in 1898 . Fi...