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HPRT in Biological News

Any exposure to tobacco smoke during pregnancy is risky

...nd sometimes even direct exposure through maternal smoking ?or produced contradictory results. The original studies looked at mutation rates at the hprt gene located on the X chromosome in cord blood samples from newborns. Dr. Grant's analysis pooled the studies' data, looking for frequency of induced ...
HPRT in Biological Technology

Protect RNA and Improve Target Cell Identification

...d to isolate RNA from the LCM sample shown in Figure 2. RNA from hprt was amplified from this sample by qRT-PCR (Figure 5A). In additi... Samples. (A) One-step qRT-PCR reaction to amplify hprt mRNA was performed with total RNA isolated from human ovary LCM sample ...

Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments

...y. Conclusion The housekeeping genes G6PDH and hprt can be used as a positive control for gene knockdown experiments when siRNA transfection conditions have to be optimized. The LightCycler Instru...

Quantification of siRNA Silencing Efficiency Using the LightCycler System

...ata from the housekeeping genes were used to normalize the GFP results (Figure 3). Of the five tested housekeeping genes (β2M, G6PDH, ALAS, hprt and PBGD) the expression level of β2Microglobulin (β2M) was closest to the expression level of GFP (Figure 3a). Therefore it was selecte...

High-Throughput Isolation of Total RNA

...cells was also subjected to molecular beacon RT-PCR analysis 5 using primers and a beacon specific for the low-abundance target hprt ( Figure 4 ). The beacon hybridizes quantitatively to the target sequence and emits fluorescence in this conformation. The thres...
HPRT in Biological Definition

Purine

...ltransferase salvages adenine, while hypoxanthine-guanine phosphoribosyltransferase (HPRT) salvages guanine and hypoxanthine. Genetic deficiency of hprt causes Lesch-Nyhan syndrome . History Purine was named by the German chemist Emil Fischer in 1884 . He synthesized it in 1898 . Fi...
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(Date:9/1/2014)... Steven Reinberg HealthDay Reporter ... a head-to-head comparison, an experimental drug was more effective ... heart failure patients. According to the study authors, ... benefit of the new drug, dubbed LCZ696. ... standard medication, enalapril (Vasotec), either died or were hospitalized ...
(Date:9/1/2014)... Watching action shows on TV may be bad for your ... snack food while watching action films and programs than something ... find that if you,re watching an action movie while snacking ... Tal, of the Cornell Food and Brand Lab, said in ... the program is, the more you will eat." The ...
(Date:9/1/2014)... September 01, 2014 Currently, scientists ... effective treatments for Alzheimer's disease (AD), which reflects ... exist regarding the diagnosis of the disease, especially ... of appropriate diagnostic tests. In 2014, after ... greatest breakthroughs in the medicine world, there are ...
(Date:9/1/2014)... York, NY (PRWEB) September 01, 2014 ... have been added to a federal litigation that continues ... South Carolina, Bernstein Liebhard LLP reports. , A ... claims now filed over the widely-used cholesterol medication by ... the drug. According to claims, Pfizer Inc. failed to ...
(Date:9/1/2014)... 2014 The B-cell non-Hodgkin’s Lymphoma (NHL) ... major markets (the US, France, Italy, Germany, Spain and ... billion by 2018. , The competitive landscape in the ... Spain and the UK is poised to undergo a ... the four largest B-cell NHL indications, which includes Diffuse ...
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(Date:8/29/2014)... type and number of connections in transcription factor networks ... in a study published in PLOS Computational Biology ... assortativity signature contributes to a network,s resilience against mutations. ... out-out assortativity of TFN models has a greater effect ... types of assortativity," said Dov A. Pechenick, PhD, lead ...
(Date:8/29/2014)... twist them, fold them: modern materials that are light, ... as artificial skin or electronic paper. , Making such ... but a new way of working with copper nanowires ... , Previous success in the field of ultra-lightweight ... precious gold and silver nanowires. , By turning ...
(Date:8/28/2014)... an expansion of marine protected areas is needed to ... According to investigators from the Wildlife Conservation Society and ... on saving the largest numbers of species, often at ... difficult-to-replace ecological functions. , Many vital ecological ... that also are food for millions of people. ...
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