Global Scientific Research Collaboration Leads to Breakthrough in Culturing HEK 293 Cells in Animal-free Media

Joint study between leading researchers in academia and industry from US, Australian and European Researchers leads to discovery of method for promoting HEK 293 cell growth and productivity using protein derived from colostrum. Fort Collins, CO (PRWEB) October 28, 2008 ...

Chronically Ill Prisoners Face Great Healthcare Problems

... homeless may find their healthcare improves//, according to a study in the latest Journal of Advanced Nursing. Researchers led by the late Gill hek from the Faculty of Health and Social Care at the University of the West of England, Bristol, spoke to a cross-section of 111 prisoners in 12 prisons ...

HEK in Biological News

Tailor-made recombinant proteins in mammals

...i and her colleagues showed that it works equally well in the most common mammalian system, CHO cells (Chinese hamster ovary cells), as well as in the hek cell system (human embryonic kidney cells). "You can put anything you want on the tagged protein," she says, "You attach small molecule drugs, or p...

HEK in Biological Technology

Cellular Dynamics hits market with UW research heavyweights

...to build its screening services around two different technologies that were developed at UW-Madison. The company's initial screening services will use hek cell techniques developed in January's lab at Madison for hERG screening, hERG channels being a type of path through the body that can be blocked, lea...

Cellular Dynamics getting $2 million in Wisconsin financing

...eening services in January, or sometime in the first quarter of 2006, using hek cells for hERG screening. hek cells are derived from kidney cells that have been modified to hve some of ...

A technique for increasing yields in bioreactors and disposable cell-culture systems

.../products/bioreactors/CelliGenPlus2. asp ). 1. Kaufman, J.B. et al . Continuous production and recovery of Ca 2+ binding receptor from hek 293 cells using perfusion through a packed bed bioreactor. Cytotechnology 33 , 311 (2000). 2. Wang, G. et al. Modified CelliGe...

The Genesis of Gendicine: The Story Behind the First Gene Therapy

... gene. Engineered cells expressing adenovirus E1 proteins are required to produce Gendicine. Usually, people use the hek 293 cell line for this purpose. We have subcloned a cell line called SBN-Cel from the 293 cell line. SBN-Cel is much better ...

Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System

.... Kaufman J.B., Wang, G., Zhang, L., Valle, M.A. and Shiloach, J. Continuous Production and Recovery of Ca2+ Binding Receptor from hek 293 Cells Using Perfusion through a Packed Bed Bioreactor. Cytotechnology 33: 3-11 , 2000. ...

Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar

... a density of 12,000 stably transfected hek 293 cells per well was plated with the Deerac Flui..., a density of 4,000 stably transfected hek 293 cells per well was plated with the Deerac Flui... mode (figure 2 and figure 3). hek 293 cells were treated with isoproterenol for 5 ho...

FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

... promiscuous G-proteins. For transient expression, hek cells seeded at an approximate density of 3x10 6 ...aximally effective concentration of 5-HT (10 M) in hek cells transiently expressing the 5-HT 2C (VSV iso... of 8,000-12,000 can be achieved with FLUO-4 using hek cells transfected with the same construct under op...

Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers

...tech has recently introduced the BD Living Colors hek 293 ZsGreen Proteasome Sensor Cell Line that allow...ctivity. 4 It was obtained by stably transfecting hek 293 cells with the Proteasome Sensor Vector (pZsPr...ents. MATERIALS hek 293 ZsGreen Proteasome Sensor Cell Line (BD Biosci...

measurement of green fluorescent proteins and a red fluorescent protein in live cells in the Analyst GT multimode reader

...aration and analysis The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 g/mL of G418. Non-transfected hek cells were included as controls. The night before the experiment, they were trypsinized and the cell suspension was diluted serially to give cell dens...

Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000

...h identifies, counts, and analyzes grain-like structures within the cells. Figure 1 shows a scatter plot for β 2-adrenergic receptor expressing hek cells, with and without isoproterenol stimulation. A signal:noise ratio of 10:1 and Z’ factor of 0.61 were obtained for this assay. ...