Cellular Dynamics hits market with UW research heavyweights
...to build its screening services around two different technologies that were developed at UW-Madison. The company's initial screening services will use hek
cell techniques developed in January's lab at Madison for hERG screening, hERG channels being a type of path through the body that can be blocked, lea...
Cellular Dynamics getting $2 million in Wisconsin financing
...eening services in January, or sometime in the first quarter of 2006, using hek
cells for hERG screening. hek
cells are derived from kidney cells that have been modified to hve some of ...
A technique for increasing yields in bioreactors and disposable cell-culture systems
1. Kaufman, J.B. et al . Continuous production and recovery of Ca 2+ binding
receptor from hek
293 cells using perfusion through a packed bed bioreactor.
Cytotechnology 33 , 311 (2000).
2. Wang, G. et al. Modified CelliGe...
The Genesis of Gendicine: The Story Behind the First Gene Therapy
... gene. Engineered cells expressing
adenovirus E1 proteins are required to
produce Gendicine. Usually, people use the
293 cell line for this purpose. We have
subcloned a cell line called SBN-Cel from
the 293 cell line. SBN-Cel is much better
Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System
.... Kaufman J.B., Wang, G., Zhang, L., Valle, M.A. and
Shiloach, J. Continuous Production and Recovery of
Ca2+ Binding Receptor from hek
293 Cells Using
Perfusion through a Packed Bed Bioreactor.
Cytotechnology 33: 3-11 , 2000.
Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
... a density of 12,000 stably transfected
293 cells per well was plated with the Deerac Flui..., a density of 4,000 stably transfected
293 cells per well was plated with the Deerac Flui...
mode (figure 2 and figure 3).
293 cells were treated with isoproterenol for 5 ho...
FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
... promiscuous G-proteins. For transient expression, hek
cells seeded at an approximate density of 3x10 6 ...aximally effective concentration of 5-HT (10 M) in hek
cells transiently expressing the 5-HT 2C (VSV iso... of 8,000-12,000 can be achieved with FLUO-4 using hek
cells transfected with the same construct under op...
Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
...tech has recently introduced the BD Living Colors hek
293 ZsGreen Proteasome Sensor Cell Line that allow...ctivity. 4 It was obtained by stably transfecting hek
293 cells with the Proteasome Sensor Vector (pZsPr...ents.
293 ZsGreen Proteasome Sensor Cell Line (BD Biosci...
measurement of green fluorescent proteins and a red fluorescent protein in live cells in the Analyst GT multimode reader
...aration and analysis
The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 g/mL of G418. Non-transfected hek
cells were included as controls. The night before the experiment, they were trypsinized and the cell suspension was diluted serially to give cell dens...
Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
...h identifies, counts, and analyzes grain-like structures within the cells. Figure 1 shows a scatter plot for β 2-adrenergic receptor expressing hek
cells, with and without isoproterenol stimulation. A signal:noise ratio of 10:1 and Z’ factor of 0.61 were obtained for this assay.