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Glycosylase in Medical News

AGI Dermatics Data Indicates 8 Oxo-Guanine Repair Enzyme OGG1 Encapsulated in Liposomes, Reduces MMP-1, Increases Collagen and Enhances DNA Repair

... of Dermatology FREEPORT, N.Y., Feb. 11 /PRNewswire/ -- AGI Dermatics presented clinical research that indicates the 8-oxo-guanine DNA glycosylase (OGG1) encapsulated in liposomes is an effective agent in treating photodamaged skin by enhancing DNA repair, reducing MMP-1 secretion and increasing ...
Glycosylase in Medical Technology

AGI Dermatics Data Shows the 8 Oxo-Guanine Repair Enzyme OGG1 Encapsulated in Liposomes, Reduces MMP-1 and TNF-alpha in UV Irradiated Skin Cells

...ive Dermatology FREEPORT, N.Y., May 16, 2007 /PRNewswire-FirstCall/ -- AGI Dermatics today announced new clinical research that the 8-oxo-guanine DNA glycosylase (OGG1) encapsulated in liposomes enhances DNA repair and reduces MMP-1 and TNF-alpha in UV irradiated skin cells. The data was presented at the 68th A...
Glycosylase in Biological News

Newly found enzymes may play early role in cancer

...shut them off and prevent these mutations?" The enzymes leading to DNA demethylation involve the coupling of a 5-meC deaminase enzyme, a G:T glycosylase enzyme and Gadd45, which is not an enzyme. ...

News tips from the Journal of Neuroscience

...nclude increased levels of homocysteine, increased uracil incorporation into DNA, and increased DNA strand breaks due to uracil excision by uracil-DNA glycosylase (UNG). To investigate which of these molecular effects contribute most significantly to neurological effects, Kronenberg et al. examined the effects o...
Glycosylase in Biological Technology

Preventing Carry-over Contamination with Uracil-DNA Glycosylase

The polymerase chain reaction (PCR) can amplify a single molecule over a billionfold. Thus, even minuscule amounts of a contaminant can be amplified and lead to a false positive result. Such contaminants are often products ...

Uracil-DNA Glycosylase

for prevention of carry-over contamination in PCR Cat. No. 1 444 646 100 units Cat. No. 1 269 062 25 units Description Uracil-DNA Glycosylase (UNG) (from E. coli) catalyzes the remo...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

...tem, the plasmid standards and breast cancer cDNA were simultaneously assayed in duplicate 15 l reactions as follows: uracil DNA glycosylase supermix (7.5 l), forward primer/probe (0.3 l), reverse primer (0.3 l), plasmid or specimen cDNA (1 l), water (5.9 l). PCR condi...

PCR Polymerases Application Profiles

...n enzyme system can be used with dUTP instead of dTTP to apply procedures for PCR carry-over prevention using uracil DNA glycosylase (UNG). ...

Trouble-shooting: Smeared bands

... minutes UNG Digestion: Incorporate d-UTP nucleotides into reaction and do subsequent uracyl DNA glycosylase digestion Back to Top Primer ...

Trouble-shooting: Non-specific bands

... minutes UNG Digestion: Incorporate d-UTP nucleotides into reaction and do subsequent uracyl DNA glycosylase digestion Back to Top Primer ...

PCR Master

...-dUTP) Prevention of carry-over contamination, by incorporation of dUTP and degradation of contaminating DNA with Uracil-DNA glycosylase Cycle sequencing Operating parameters pH optimum: approx. ...

C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR

...pecificity of transcription for GC-rich templates. Accepts DIG-labeled and other modified nucleotides. Works with Uracil-DNA glycosylase (PCR carry-over prevention). Key advantages Enhances specificity, becau...

Product overview

...lity enzyme blend. Uracil-DNA glycosylase Uracil-DNA glycosylase is used for prevention of carry-over contamination in PCR. The e...

Reverse transcriptases suitable for two-step RT-PCRd

..., and improved sensitivity. c For prevention of carry-over contamination with Uracil-DNA glycosylase (UNG) during One-Step RT-PCR, we recommend using the C. therm. DNA Polymerase One-Step RT-PCR System for products up to ...
Glycosylase in Biological Dictionary

Uracil

...acil perform important functions ... uracil DNA N-glycosylase activity. • protein binding ... Structure of the gene for human uracil -DNA glycosylase and analysis of the promoter function. ... Uracil pairs with adenine through hydrogen bonding. Uracil is the hydrogen bond acceptor and c...
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