What's in your water?: Disinfectants create toxic by-products

...ewa, along with a team of scientists received a United States Environmental Protection Agency Science and Technology award for their paper Occurrence, genotoxicity and carcinogenicity of regulated and emerging disinfection by-products in drinking water: A review and roadmap for research. It was published in the s...

TetraQ recognized for toxicology services

...ervice delivery which includes acute and chronic toxicology services, said Professor Smith. These toxicology services include cytotoxicity assays, genotoxicity assays - bacterial mutation (Ames) tests; mouse lymphoma assay; mammalian erythrocyte micronucleus testing; dose range finding; safety pharmacology (c...

iNOS expression may links chronic biliary inflammation to malignant transformation

...ne stimulation induced iNOS expression and NO generation, which was sufficient to cause DNA damage. The results of this study suggest that NO-mediated genotoxicity induced by inflammatory cytokines through activation of iNOS is involved in the process of biliary carcinogenesis in response to chronic inflammation ...

Nanoparticles can Damage DNA, Increase Cancer Risk

...ooked at how two different types of nanoparticles could cause DNA damage in the MCF-7 line of breast cancer cells. She and her team examined the genotoxicity of silica and C60 fullerene nanoparticle suspensions using the alkaline single-cell gel electrophoresis assay (Comet assay) to quantify breaks in sing...

Grant Encourages Drug Testing Sans Animals

...– awarded by the National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) – will help the scientists establish new genotoxicity tests using cultured human liver cells. It is hoped the new test will not only reduce the number of compounds that are tested on animals but also ens...

Detecting DNA Damage With In-Vivo Alkaline Comet Assay

...tissues from the same animal. In conclusion, a further fully validated genotoxicity assay has been added to the battery of genotoxicity tests available at RCC. Refinement of the technique will continue as experi...

Genotoxicity in Biological News

Cancer tip -- Nanoparticles can damage DNA, increase cancer risk

...oked at how two different types of nanoparticles could cause DNA damage in the MCF-7 line of breast cancer cells. She and her team examined the genotoxicity of silica and C60 fullerene nanoparticle suspensions using the alkaline single-cell gel electrophoresis assay (Comet assay) to quantify breaks in sing...

New research to cut animal testing

...g ?awarded by the National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) ?will help the scientists establish new genotoxicity tests using cultured human liver cells. It is hoped the new test will not only reduce the number of compounds that are tested on animals but also ...

Any exposure to tobacco smoke during pregnancy is risky

...r secondary ?exposure causes just as much damage as active smoking, and it is the same kind of damage," said Dr. Grant, whose primary area of study is genotoxicity and the mechanisms of DNA repair. "These kinds of mutations are likely to have lifelong repercussions for the exposed fetus, affecting survival, birth...

Genotoxicity in Biological Technology

BioReliance Launches Next-Generation Genotoxicity Screening Service

ROCKVILLE, Md., Sept. 12 /PRNewswire/ -- BioReliance Corporation, a leading contract services organization for the biopharmaceutical and pharmaceutical industries, has contracted with Gentronix to offer their GreenScreen HC in vitro assay as a major part of BioReliance's portfolio of genotoxic...

Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats

Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats Precise and cost-effective micronucleus analysis made easy with the icroFlow Micronucleus Analysis Kit Dorothea K. Torous Carol R. Tometsko Litron Laboratories, Rochester, N...

Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

...om typical assays carried out using the protocol described are shown in Figure 4. Exposure of cells to increasing concentrations of compounds of known genotoxicity results in an increase in the percentage of bi-nucleate cells with micronuclei. As cells are exposed to higher doses of compounds, cell cycle inhibiti...

Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer

...s are generally induced in the same tissue that tumors are produced but not in nontarget tissues. This makes the transgenic model for the study of genotoxicity even more valuable, since mutations can be studied in virtually any tissue. The hallmark of occupational- and lifestyle-related cancer is tissue...

RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

...R techniques to study gene expression in areas such as genetic toxicology, where changes in the regulation of these genes may be indicative of the genotoxicity of a substance or its biologically activated form. The primer sets may also be used to quickly amplify a region of the featured genes for later us...

Genotoxicity in Biological Dictionary

Recombinase

...Cre recombinase catalyzes DNA strand exchanges in palindromic DNA target ... to as recombinase -mediated cassette exchange, ... CRE RECOMBINASE genotoxicity ... This feature of recombinase activity is similar to the mechanism of type IB ... that SUMO attachment to Flp recombinase prevents Flp from c...

Micronucleus

...ntific Cellomics products automate drug discovery through a unique, cell-based assay platform that ... in vitro micronucleus assay is among ... genotoxicity : THE MICRONUCLEUS TEST. A micronucleus (MN) is formed during the ... The combination of the micronucleus assay with fluorescence in situ ... ...