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Geneticin in Biological Technology

Calcium Flux Assays using the LabChip 3000

...grown in Ham's F12 medium adjusted to contain 1.5 g/L sodium bicarbonate with 100 U/mL penicillin, 0.1 mg/mL streptomycin, 2 mM L-glutamine, 0.1 mg/mL geneticin G418, 1 mM sodium pyruvate, 10 mM HEPES and 10% Fetal Bovine Serum. Cells were maintained at 37 oC and 5% CO 2 and subcultured in monolayer...

Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

... solution (Invitrogen Life Technologies, cat # 15630-056), FBS: (Fetal Bovine Serum, heat inactivated, Invitrogen Life Technologies, cat # 10099-141), geneticin (G418, Invitrogen Life Technologies, cat # 10131-019). Fluo-3 and Fura-2 reagent preparation ...

Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors

..., and pindolol from RBI chemicals. Hams F-12 media, foetal bovine serum and geneticin 418 were obtained from Gibco BRL. Method CHO cells ex...ce in Hams F-12 media containing 10% foetal bovine serum and 500 μg/ml geneticin 418, harvested and resuspended in stimulation buffer, containing a phosphod...

The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies

... HEK293 cell line expressing EGF receptor HEK Culture medium: MEM (Sigma M2279) containing: 10% (v/v) FBS geneticin G418, 200 µg/ml (Sigma) L-glutamine (Gibco) Non-essential amino acids (Sigma)

Assay medium: MEM, phenol red ...

The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR

...Sigma) containing 10% (v/v) FBS (Sigma), 200 µ g/ml geneticin G418(Sigma), L-glutamine (Gibco), Non-essential amino... containing 10% (v/v) FBS (Sigma), 200 µ g/ml geneticin G418 (Sigma), L-glutamine, penicillin-streptomycin, (Gibco) ...

Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000

...ged β2-adrenergic receptor

Culture medium: MEM (Sigma) 10% (v/v) FBS (Sigma) 200 µg/ml geneticin 418 (Sigma) L-glutamine (Gibco-BRL) Non-essential amino acids (Sigma)

Assay medium: MEM, phenol red free (In...

Rapid hit confirmation and early hERG liability testing using the IonWorks HT system

... Cat. #11965-092) 10% Fetal Bovine Serum (FBS, Irvine Cat. #3000), 1% Penicillin-Streptomycin (Irvine Cat. #9366) and 1% mL geneticin (G418, Gibco Cat. #10131) PatchPlate consumables (Molecular Devices Cat. #9000-0688) Compound plates (IonWorks HT assay): Cost...
Geneticin in Biological Products

GIBCO Geneticin Selective Antibiotic liquid from Invitrogen

Description: Geneticin Selective Antibiotic liquid...
Company:Invitrogen

pPIC9K from Invitrogen

Description:...namycin resistance gene that confers resistance to geneticin Reagent in Pichia. Spontaneous generation of multi...be identified by resistance to increased levels of geneticin Reagent. Pichia transformants are selected on hist...dium and screened for their level of resistance to geneticin Reagent. The ability to grow in high concentration...
Company:Invitrogen
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