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GAPDH in Medical News

Key Protein Might Shield Brain Cells

... with another protein when it attempts to latch onto a multifunctional molecule called glyceraldehyde-3-phosphate dehydrogenase (GAPDH). By binding to gapdh itself, GOSPEL prevents the cell death cascade and protects brain cells against toxic compounds, according to the study. The finding is published i...

Cells Re-Energize to Come Back From the Brink of Death

...apoptosis increases the levels of an enzyme called gapdh in order to counteract the backup program called caspase-independent cell death (CICD). gapdh appears to prevent CICD by supporting the function... The findings also suggest that the increase in gapdh provides energy to increase autophagy -- the proce...

Basis for Nerve fiber Damage in Multiple Sclerosis Discovered

...nd in spinal fluid bound to two specific enzymes – gapdh and TPI. These two enzymes are essential for ef...th. In addition to the energy-production function, gapdh is involved with a number of genetic activities, s...t studies have shown that binding of inhibitors to gapdh and TPI causes decreased ATP production in neurons...
GAPDH in Biological News

Cells re-energize to come back from the brink of death

...apoptosis increases the levels of an enzyme called gapdh in order to counteract CICD. gapdh appears to prevent CICD by supporting the function...ge. The findings also suggest that the increase in gapdh provides energy to increase autophagy—the process ...
GAPDH in Biological Technology

Paper in Cell Cycle Reports Alfacell's ONCONASE(R) Targets siRNA

... expressed housekeeping gene) in human lung adenocarcinoma A549 cells by siRNA was effectively prevented by ONCONASE. While transfection of cells with gapdh siRNA reduced expression of this protein by nearly 70 percent, the expression was restored in the cells exposed to ONCONASE for 48 or 72 hours. The da...

Measuring siRNA-mediated knockdown of IL-8 mRNA

... IL-8 expression. Based on the experimental results (data not shown), we determined that Cyclophilin B (PPIB) demonstrated more stable expression than gapdh and subsequently was used as the normalization control. Following stimulation and siRNA transfection, the mRNA levels of each gene were measured from ...

Amplification Efficiency of TaqMan Gene Expression Assays

...er dilution ranges. The initial data from the first 30 assays and gapdh allowed us to design the optimal protocol for measuring amplification effic...anscript (NHT) as an invariant endogenous control. The results obtained for gapdh are shown in Figure 5. The fold change results obtained using the Δ ...

Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

...fication of total RNA samples is a widespread problem with other total RNA extraction methods. The ABI PRISM 7700 Sequence Detection System and an RT+ gapdh based assay easily detects the presence of such inhibitors. Applied Biosystems total RNA lysis reagents and purification technology readily removes th...

Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data

... and the resultant averaged cycle threshold (C T ) was determined. Fold changes for the TaqMan assay were calculated using relative quantitation using gapdh gene expression and the delta CT method (delta C T = delta C T tumor-delta C T T normal). Expression Array System fold change was determined by calc...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

...and reagents as presented in Table I. The epsilon- and gamma- subunit of the acetylcholine receptor and slow MyHC are amplified. The housekeeping gene gapdh was amplified as a reference for the amount loaded and the quality of the cDNA. All primers (Table II) are intron spanning to produce a cDNA specific ...

Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

...YL-3', Operon) 0.15 l 100 M gapdh forward primer (5'-CATGTTCCAATATGATTCCAC-3', Operon) 0.15 l 100 M gapdh reverse primer (5'-CCTGGAAGATGGTGATG-3', Operon) 0.1 l 100 M gapdh probe (5'-HEX-CAAGGCTGAGAACGGGAAGCTTGTCCAGCCTTG- ...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

...-cDNA II reverse transcription reactions to amplify both DBH and gapdh as an internal control for sample normalization (Figure 1). No D...tion were performed with the Cells-to-cDNA II Kit (A) . DBH and gapdh mRNAs were quantified using real-time PCR. DBH values were norma...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

...-cDNA II reverse transcription reactions to amplify both DBH and gapdh as an internal control for sample normalization (Figure 1). No D...tion were performed with the Cells-to-cDNA II Kit (A) . DBH and gapdh mRNAs were quantified using real-time PCR. DBH values were norma...

Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs

... Figure 2. Effect of HCV-specific siRNAs on HCV and gapdh RNA Transcript Levels. Total RNA was isolated from S11791 cells ...sferred to membrane and hybridized with probes to HCV and GAPDH. gapdh signal was used for normalization of the HCV signal. ...
GAPDH in Biological Products
GAPDH in Biological Dictionary

Transfection

...fluencing Successful Transfection . 9.1 Concentration and purity of nucleic acids ... Twenty-four hours post transfection ,cells were lysed and gapdh and cyclophillin ... Theincubation time with the transfection complex, serum-free media and cells was ... Our online cell database — customer...
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