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GAL4 in Biological Technology

Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators

Stable reporter cell lines for signal transduction pathway readout Lisa Hexdall Li Xu Alan Greener Mary Buchanan John Bauer Chao-Feng Zheng Stratagene Stratagene now offers the PathDetect HLR-CREB, HLR-Elk1, and HLR-cJun, cell lines F ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

...c plasmid ( Figure 1 ) is the pFR-Luc reporter plasmid of the PathDetect cis-reporting system. To this backbone, we replaced the gal4 binding element with four direct repeats of the NFAT binding sequence (286 to 257) from the IL-2 gene promoter, the most studied...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

...plasmid ( Figure 1 ) is the pFR-Luc reporter plasmid of the PathDetect trans-reporting system. To this backbone, we replaced the gal4 binding element with four direct repeats of the NFAT binding sequence (286 to 257) from the IL-2 gene promoter, the most studied...

New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways

...ific trans-activator proteins fused to the yeast gal4 DNA binding domain. To facilitate the construction...n of any transcription factor fused to the yeast gal4 DNA binding domain. The pFA-CMV plasmid features a... (constructed with the pFA-CMV plasmid) expressing gal4 fusions with c-Fos (208-313) and ATF2 (1-96). ...

Improved Vectors for PathDetect Trans-Reporting Systems

...ed by a synthetic promoter that contains the yeast gal4 upstream activating sequences (UAS) (3) a positi...ion factor sequence and subsequent expression of gal4 fusion proteins in a variety of mammalian cells. T... of improved vectors. This plasmid expresses the gal4 DNA binding domain without a transcriptional act...

In Vivo Signal Transduction Pathway Reporting Systems

...ll three fusion trans-activator plasmids share the gal4 DNA binding domain 5,6 but have differ... factor in mammalian cells can bind the gal4 binding element efficiently, the use of gal4 binding elements in the reporter vectors minimizes...

Optimizing Transfection Conditions for Studying Signal Transduction Pathways

...ans-activator proteins that consist of the yeast gal4 DNA binding domain and the activation domains of v... species of the GAL4-ATF2 fusion compete for the gal4 binding sites on the reporter plasmid. Because t...ssing the MEKK protein and binds to the gal4 binding domain. However, in this scenario, too muc...

Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems

... of the yeast transcriptional activator gal4 6,7 ; the pFR-Luc reporter plasmid for expression ... by a synthetic promoter that contains the yeast gal4 binding sites; a positive control plasm...th the pFC2-dbd plasmid, which contains only the gal4 DNA binding domain, little or no luciferase is e...

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

..., we constructed a set of user-friendly gal4 BD (binding domain)-bait and NF- k B AD ...ase) located downstream of the multiple gal4 DNA-binding sites. The gal4 BD-bait, NF- k B AD-target, and a repor...
GAL4 in Biological Products

MaV203 Competent Yeast Cells Library Scale from Invitrogen

Description:...Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous gal4 and GAL80 genes for use with GAL4-based two-hybrid systems. The strain also...ions for selection of ProQuest bait and prey vectors. MaV203 contains three gal4 inducible reporter genes, providing four phenotypes, for easier identificat...
Company:Invitrogen

MaV203 Competent Yeast Cells Subcloning Scale from Invitrogen

Description:...Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous gal4 and GAL80 genes for use with GAL4-based two-hybrid systems. The strain also...ions for selection of ProQuest bait and prey vectors. MaV203 contains three gal4 inducible reporter genes, providing four phenotypes, for easier identificat...
Company:Invitrogen
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