Uses and Applications of FuGENE 6 Transfection Reagent
Protocol Instructions for Users Who are New to Transfection Below, a standard protocol for the DNA transfection of cultured cells using FuGENE 6 is presented that has been specially designed for scientist...Uses and Applications of FuGENE 6 Transfection Reagent
Specific technique considerations Co-transfections First, verify that both of your DNA constructs work in your cell line. Also, determine the ratio of DNA to FuGENE 6 Reagent (for e...Uses and Applications of FuGENE 6 Transfection Reagent
Optimization Tips Refer to the following table if you observe low transfection efficiency. Possible Cause Recommendation Nucleic acids of...Uses and Applications of FuGENE 6 Transfection Reagent
Application of FuGENE 6 Transfection Reagent in Vector-based shRNA Experiments RNA interference induced by siRNAs or shRNAs allows reliable down-regulation of the expression of specific g...Uses and Applications of FuGENE 6 Transfection Reagent
Troubleshooting Hints if You Observe Cytotoxicity (greater than 5 - 10 % Cell Death) FuGENE 6 Transfection Reagent has proven to be virtually nontoxic to most cell types. If you observe more than 5 - 10...