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Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM

Description:This quick (10 min) assay can detect H2O2 or peroxidase activity utilizing a non-fluorescent substrate, 10-acetyl-3,7-dihydroxyphenoxazine (ADHP). H2O2 oxidizes ADHP in a 1:1 stoichiometry to produce a fluorescent product resorufin (excitation at 530-571 nm, emission at 590-600 nm). This reaction is catalyzed by peroxidase in a one-step, homogeneous, no wash assay system. ADHP can be utilized to...
Company:BACHEM

20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON

Description:The ubiquitin-proteasome pathway is the major proteolytic system in the cytosol of eukaryotic cells, where it catalyzes the selective degradation of short-lived regulatory proteins and the rapid elimination of proteins with abnormal conformation (Hershko & Ciechanover 1998; Hochstrasser 1996). The critical protease in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which...
Company:CHEMICON

Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem

Description:The InnoCyte Laminin-Based 96-well Cell Invasion Assay is designed to determine the invasive capacity of cells using laminin as a significant barrier to invasion or to screen potential anti-metastatic agents in vitro in a convenient, high-throughput, 96-well format. The 8 mm pore size is appropriate for all types of adherent cells such as epithelial, endothelial, or fibroblast cells. The assay is...
Company:Calbiochem

Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON

Description:...
Company:CHEMICON

Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

Description:Molecular Probes EnzChek Pyrophosphate Detection Kitprovides a fast, convenient and inexpensive spectrophotometricmethod for measuring the inorganic pyrophosphate (PPi ) producedby a number of biochemical reactions, such as DNA andRNA polymerizations, cyclic AMP formation by the enzymeadenylate cyclase and the enzymatic activation of fatty acids toform their coenzyme A esters.1 The EnzChek...
Company:Molecular Probes (Invitrogen)

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Fluorometric at Biology Technology

Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

Evelyn McGown, Ph.D. and Anna Lam, B.S. Molecular Devices Corporation, 8/99 Modern assays for proteolytic activity typically use synthetic chromogenic or flu-orogenic peptide substrates. Enzyme selectivity is obtained by choosing a peptide sequence uniquely recognized by the catalytic site of a particular enzy...
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