Stanford researchers harness nanoparticles to track cancer-cell changes
...ent dye molecules to antibodies, with different colors tied to antibodies that target different molecules. The dye molecules respond to laser light by fluorescing
echoing light at exactly the same wavelength, or color, with which they were stimulated. The fluorescence's strength indicates the abundance of the c...
Glowing Substance Lights Up Cancer in Mice
...ble fluorescent compounds, our compound consists of a targeting agent and a fluorescing
agent that act independently. We can target the fluorescing
agent to different types of cancer cells by using any antibody or molecule ...
MBL scientist Osamu Shimomura wins Nobel Prize for discovery of green fluorescent protein
...f University of California, San Diego.
Dr. Shimomura, prior to his retirement, was an MBL senior scientist from 1982-2001. He first noticed a green fluorescing
molecule in the jellyfish Aequorea in 1961. He patiently extracted the molecule from 10,000 specimens, purified it, and identified as a protein. The ...
Sound medicine at Acoustics Meeting
...and his colleagues at Swarthmore College have shown that acoustical waves can disrupt biofilms and destroy bacteria. They grew biofilms of a strain of fluorescing
E. coli on microscope slides sandwiched between piezoelectric wafers. This allowed them to view the biofilm under a confocal microscope before, during...
Fluorescing in Biological News
Boston University biomedical engineers teach bacteria to count
... network translates and transcribes the gene for a fluorescing
protein, which is visible to researchers.
The s...successive flip-over counts another event, and the fluorescing
protein is activated after the third one.
In bo...o count to higher numbers, or additional genes for fluorescing
proteins might be added, for example, to glow red ...
Penn researchers demonstrate a new model for drug discovery with a fluorescent anesthetic
...the search for safer and more effective drugs.
The study is published online in the Proceedings of the National Academy of Sciences .
Using the fluorescing
compound 1-aminoanthracene, or 1-AMA, the team developed a high-throughput assay to test for new anesthetic compounds. The assay will allow researche...
New high-throughput screening technique makes probing puzzling proteins possible
...e to which that light is polarized, in other words how well aligned those waves of emitted fluorescent light are, is largely determined by how big the fluorescing
molecule is. Smaller molecules rotate more rapidly, causing their emitted light to be less polarized, so light from larger molecules is measurably mor...
Researchers create new class of fluorescent dyes to detect reactive oxygen species in vivo
...ry in their ability to detect intracellular or extracellular reactive oxygen species and by their emission wavelength from 560 to 830 nanometers.
at higher wavelengths allows the hydrocyanine dyes to be used for deep tissue imaging in vivo, a capability that dihydroethidium (DHE), the current "g...
...rs during ripening. In this process, colorless but fluorescing
breakdown products of chlorophyll are concentrated...d could explain the unusually long duration of the fluorescing
intermediates in bananas. fluorescing
chlorophyll catabolytes have otherwise only been f...
Fantastic photographs of fluorescent fish
... Scientists have discovered that certain fish are capable of glowing red. Research published today in BMC Ecology includes striking images of fish fluorescing
vivid red light.
Due to absorption of 'red' wavelengths of sunlight by sea-water, objects which look red under normal conditions appear grey or bla...
Automated microfluidic device reduces time to screen small organisms for genetic studies
...timing of appearance of a fluorescent protein in a population of organisms. They were able to sort the free-moving animals into two categories, those fluorescing
in a particle neuron and those that are not, at a speed of approximately 900 animals per hour. More than 90 percent of the animals were loaded into th...
New technique yields more detailed picture of chromatin structure
All this combined gives us a much better signal, a much stronger signal, with the very best structural preservation, Kireev said.
protein helped the researchers find the regions of interest in the cells. These areas were then immunogold labeled and targeted for electron microscop...
Researchers visualize complex pigment mixtures in living cells
...there is little spectral overlap between different fluorescing
pigments. The new method, hyperspectral fluorescen...d more evenly throughout the cell, suggesting that fluorescing
phycobilisomes are more prevalent along the outer ...ach in in vivo localization of complex mixtures of fluorescing
compounds at high resolution, Vermaas said.
Catching cancer's spread by watching hemoglobin
...nstruct 3-D images of blood vessels.
A major advantage of the technique is that it is "label-free," meaning it does not require the addition of fluorescing
molecules but rather relies on the inherent light-absorbing properties of hemoglobin. While the technique has been demonstrated in vitro (by excising ...
Fluorescing in Biological Technology
Rapid heating prepares energy-saving zeolite for greater role in industrial separations
...er and Tsapatsis were among the first teams to use the technique to characterize zeolite membranes by selectively labeling grain boundary defects with fluorescing
molecules. The zeolite they study is silicate-1, one of the more than 170 frameworks of zeolite, which has pores measuring .56 nm in diameter.
Genetically engineered bacteria compute the route
... the bacteria to enable them to find a Hamiltonian path in a three-node graph. Bacteria that successfully solved the problem reported their success by fluorescing
both red and green, resulting in yellow colonies.
Synthetic biology is the use of molecular biology techniques, engineering principles, and mathema...
Rice rolls out new nanocars
...monitoring an area 10-by-10 microns square showed the cars, which appear as fluorescing
dots, zigging and zagging on a standard glass slide. Link said the cars mov... images, and the dots that move are winners. The ones that don't are either fluorescing
molecules sitting by themselves or nanocars stuck in park.
The dye tet...
Molecular hula hoop
...researchers attached one end of the rod to a glass support. To observe the rotational motions of the cuff around the sleeve, the scientists attached a fluorescing
side chain to the cuff as a probe.
To observe the rotation of the ring around the rod, the researchers used a microscopic technique called defocused...
A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology
...equisite groups that provide visible color and permit molecular binding to a material. A fluorophore (fluorochrome) is a fluorescence agent capable of fluorescing
under illumination. Some fluorescence agents absorb only in the ultraviolet and therefore are colorless when viewed in visible light.
Screening and classifying hazardous waste samples
...ction, identification, and quantification for these ubiquitous chemicals, especially in field work. The same holds true for other important classes of fluorescing
hazardous chemicals, among them organochlorine pesticides, dioxins, and polynuclear aromatic molecules.
back to top ...
Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
...number and an equal distribution
of microprojectiles in the worms (Figure 4A). Confocal laser scanning
microscopy revealed green fluorescing
signals occurring at the dorsal
tegument (Figure 4B) as well as in the anterior and posterior portions
of the worms (Figure 4C)....
Versatile Reporter Vectors for Monitoring Viral Transduction
* Titer = (fraction of cells fluorescing
over background) x (supernatant dilution/ml) x (2 x 10 5 cells infected). Titers for pFB-Neo-LacZ vector = (fraction blue cells / total # cells in fi...
Fluorescing in Biological Products
MitoLight Mitochondrial Apoptosis Detection Kit from CHEMICON
Description:...rescence (l em = 585-590 nm). In apoptotic cells with altered mitochondrial membrane potential, the dye in its monomeric form stays in the cytoplasm, fluorescing
green (l em = 527-530 nm), providing a ready discrimination between apoptotic and nonapoptotic cells. The fluorescence can be observed by fluorescenc...