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Fluorescently in Medical News

DaVita Study Demonstrates Clinical Application of Sysmex Reticulocyte Hemoglobin Equivalent (RET-He) Parameter

...parameter, which quantifies the hemoglobin content of reticulocytes. Sysmex technology uses proprietary polymethine dyes that have an affinity to, and fluorescently label, nucleic acids. Detection and characterization of immature cells may well be a more valuable approach than measuring storage or transport protei...

JCI online early table of contents: April 13, 2009

...hem ideal for in vivo imaging studies. In the study, the researchers generated zebrafish expressing a mutant form of human TAU associated with FTD and fluorescently labeled the cells expressing this protein, which enabled them to image the brain cell death accompanying TAU accumulation. A compound that could inhib...

Angiogenesis inhibitor improves brain tumor survival by reducing edema

... resulted from reduction of edema, which has significant consequences within the brain, or from a direct anti-tumor effect. The researchers implanted fluorescently labeled human or rat glioblastoma cells into the brains of mice and directly observed the tumors and surrounding tissue through transparent windows th...

Snoring or soaring? Strength of fruit-fly immune system varies

...es during resting periods ("nighttime") also survive significantly longer than those infected during active periods ("daytime"). Further, by injecting fluorescently labeled dead bacteria into flies at different points in their circadian cycle, the investigators could see increased phagocyte function at night for t...

American Chemical Society's weekly PressPac -- Jan. 23, 2008

...t on ways to avoid recombination errors, which underpin cancer, aging, and other health problems. In the study, scientists observed the behavior of fluorescently tagged DNA strands placed in water that contained no proteins or other material that could interfere with the experiment. Strands with identical nucle...

Researchers find new way to block destructive rush of immune cells

...e T cell migration, with MyH9 as the mechanical link. Captured images show fluorescently tagged actin (which partners with LFA-1 to grip the surface) gathering at the front end of the cell, and fluorescently tagged MyH9 gathering at the tail end in cycles, each time the cell takes a...

Cold Spring Harbor Protocols features innovative methods for embryology research

...hich are freely accessible online ( www.cshprotocols.org ), describe how to fluorescently tag cells in very young embryos. These cell-tagging techniques, combined w...attached to a fluorescent marker, which allows the researchers to track the fluorescently labeled cells using imaging techniques. Lansfords group uses this method to...

Novel 3-D cell culture model shows selective tumour uptake of nanoparticles

...haviour of cells in culture is similar to that seen in real life. The work was mainly carried out by graduate student Weina Meng who formulated the fluorescently labelled nanoparticles and studied them in a variety of tumour and brain cell cultures. Her early studies showed faster uptake of nanoparticles into t...

New Tumor Markers for Pancreatic Cancer

...s. We analysed biopsies of PC as well as samples of healthy pancreas. The RNA (thousands of messages sent by cells) from each sample was extracted and fluorescently labelled. The RNA has the ability to join complementary DNA. The organized DNA fragments contained on a microarray can detect all possible mes...

Simple Test Detects Genetic Signs of Lung Cancer in a Patient’s Sputu

...days. Investigators then examined the sputum with both traditional cytology and with fluorescent in situ hybridization (FISH). The FISH technique uses fluorescently labeled single-strand DNA probes to bind to the complementary strand of a specific gene. The presence, or absence, of a fluorescent signal produced wh...
Fluorescently in Medical Technology

Rapid Test for Pathogens Developed By K-State Researchers Could Be Used to Detect Diseases Used By Bioterrorists

...e 45 most common antibiotics used to treat human and animal illnesses caused by these pathogens. When a sample is submitted, technicians extract and fluorescently label total DNA, and run a microarray to check whether a particular gene is present. Narayanan said the next step will be to develop a test that indic...
Fluorescently in Medical Products

CellTracks® Analyzer II

Description:... The CellTracks® Analyzer II is a semi-automated fluorescence microscope used to enumerate fluorescently labeled cells that are immunomagnetically selected and aligned. The product consists of the CellTracks® Analyzer II, a dedicated computer loaded w...
Company:Immunicon Corporation
Fluorescently in Biological News

Researchers capture bacterial infection on film

...llow the progress of infection in real-time with living organisms. The researchers used developing fruit fly embryos as a model organism, injecting fluorescently tagged bacteria into the embryos and observing their interaction with the insect's immune system using time-lapse confocal microscopy. The research...

Microarray analysis improves prenatal diagnosis

...f DNA embedded on a glass slide to form the array, which is then exposed to fluorescently labeled single-stranded DNA. Half of the labeled DNA is from the fetus bein...color. The other is reference DNA, which is labeled with another color. The fluorescently labeled DNA reference and patient binds to DNA on the array. The color of...

Colorful spy tactics track live cells supporting cancerous tumors

...In order to watch the activity of these cells, researchers injected fluorescent dyes near tumors in mouse models of breast cancer which also expressed fluorescently tagged cells A specially designed microscope allowed live imaging of tumor-associated cells for more than 12 hours, while retaining the high resolut...

Rapid test for pathogens developed by K-State researchers

... 45 most common antibiotics used to treat human and animal illnesses caused by these pathogens. When a sample is submitted, technicians extract and fluorescently label total DNA, and run a microarray to check whether a particular gene is present. Narayanan said the next step will be to develop a test that indic...

'Telepathic' genes recognize similarities in each other

...in involvement. According to the theory, this recognition mechanism is stronger the longer the genes are. The researchers observed the behaviour of fluorescently tagged DNA molecules in a pure solution. They found that DNA molecules with identical patterns of chemical bases were approximately twice as likely to...

Looking through the eyes of a mouse, scientists monitor circulating cells in its bloodstream

...l tissue in the back of the eye. There they were able to detect circulating fluorescently labeled cells as they wound their way through the mouse. "We could detec...eriments, they monitored a million circulating cells in the mouse that were fluorescently labeled with a marker that allowed them to be spotted with a microscope. Th...

Iowa State researchers develop technology for early detection of viruses

...use small contaminants to create test errors. Yeungs single molecule spectroscopy technique involves creating chemical reagents that recognize and fluorescently tag the genetic sequence of the human papillomavirus. Test samples pass through a laser beam that lights the tags. Cameras capture the images for comp...

Novel 3-D cell culture model shows selective tumor uptake of nanoparticles

... behavior of cells in culture is similar to that seen in real life. The work was mainly carried out by graduate student Weina Meng who formulated the fluorescently labeled nanoparticles and studied them in a variety of tumor and brain cell cultures. Her early studies showed faster uptake of nanoparticles into tum...

Bursts of waves drive immune system 'soldiers' toward invaders

...rophils toward a chemical signal made by invading microbes. The researchers fluorescently tagged Hem-1 to view its dynamic propulsive power under the microscope. ...h their observations and experiments. In the research, the team used the fluorescently tagged Hem-1 to determine whether the protein participated in the wave acti...

Imaging techniques permit scientists to follow a day -- or four -- in the life of a cell

.../www.cshprotocols.org/cgi/content/full/2007/1/pdb.prot4657 , details a procedure to inject proteins into individual cells. The injected protein can be fluorescently labeled, so scientists can track the glowing protein with a microscope and observe what it's doing and where it's going inside the cell. This microinj...
Fluorescently in Biological Technology

Glowing zebrafish help researchers track role of sugars in the cell

...ornia, Berkeley, researchers have made glow-in-the-dark fish whose internal light comes from the sugar coating on their cells. This novel method of fluorescently tagging the sugar chains, or carbohydrates, that coat cells is a new tool for those studying development in the zebrafish, a laboratory organism popul...

Kodak in vivo imaging system: precise coregistration of molecular imaging with anatomical X-ray imaging in animals

...Fig. 2c ). Combined multiwavelength fluorescence and X-ray imaging is shown in Figure 3 . We injected three different fluorescently tagged imaging agents subcutaneously into different regions of the mouse abdomen. Fluorescence imaging with different filter set...

A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

...lect light. For example, unlabeled cells can be detected in a population of fluorescently labeled cells, or the interactions between fluorescently labeled cells growing on an opaque and patterned substrate can be imaged. 5...

Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers

...tude of the signal is directly proportional to the extent of binding, to a first approximation 10,11 . Materials and Methods All fluorescently labeled peptides listed below in Table 1 are available from PerkinElmer Life Sciences. They are provided as off-the-shelf reagents accompanied by buff...

Quantitative Analysis of the Cell Cycle in Imaging Applications

...rated into newly synthesized DNA by cells entering into and progressing through the S phase of the cell cycle. The drop in choice for this assay was a fluorescently labeled anti-Ki-67 antibody. Ki-67 is present in all cycling cells but is not present in quiescent cells (G0). The Ki-67 staining pattern is variable ...

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

...ion: opening the frontier to molecular analysis. Trends Genet 14:272-6, 1998. 3. Bhattacherjee et al., Laser capture microdissection of fluorescently labeled embryonic cranial crest cells. Genesis 39:58-64, 2004. 4. Chalfie M et al., Green fluorescent protein as a marker for gene expression...

Microgenomic Expression Profiling

...674; Ensure sample homogeneity for accurate nucleic acid quantitation when using the NanoDrop ND-1000 spectrophotometer. ◊ Protect fluorescently tagged nucleotide conjugates from long exposures to light. 1. Laser Cut and Laser Capture Microdissection: Isolate speci...

GenomiPhi amplification of FFPE tissue DNA for array CGH

...Human genomic DNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue samples was amplified using the GenomiPhi™ DNA Amplification Kit, fluorescently labeled with Cy™3 and Cy5 dyes, and hybridized to human BAC microarrays. Commercially available human BAC arrays from Spectral Genomics were cho...

Ettan DIGE Imager

...or data transfer

Principle of operation Upon excitation by the metal halide lamp, light is emitted from a fluorescently labeled sample in proportion to the amount of labeled compound in the sample. Emitted light is collected and converted to an electronic signal in a CC...

Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox

...th IN Cell Developer Toolbox allows quantitation of neurite extensions from fluorescently labeled cells imaged by an IN Cell Analyzer 1000 or 3000.

The pro...d neurite outgrowth was measured by quantitation of neurite extensions from fluorescently labeled neuronal cells. In the indirect immunoassay used here, FITC-conjuga...

Fluorescently in Biological Products

FAM FLISP™ Lys-CMK Assay Kit from B-Bridge International

Description:...bull; Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activity in whole living cells. Select from several cell-permeable serine...
Company:B-Bridge International

SR-101 FLISP FLISP Leu-CMK Assay Kit from B-Bridge International

Description:...bull; Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activity in whole living cells. Select from several cell-permeable serine...
Company:B-Bridge International

FAM FLISP™ Leu-DAP Assay Kit from B-Bridge International

Description:...bull; Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activity in whole living cells. Select from several cell-permeable serine...
Company:B-Bridge International

FAM FLISP™ Spacer-Leu-CMK Assay Kit from B-Bridge International

Description:...bull; Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activity in whole living cells. Select from several cell-permeable serine...
Company:B-Bridge International

Veritas Microdissection Instrument, with LCM and Laser Cutting from Arcturus, now part of Molecular Devices

Description:...sitivity, variable integration time, color CCD video camera and red, blue and green filter cubes for the easy identification and precise dissection of fluorescently stained cells. Custom UV filter cubes are also available....
Company:Arcturus, now part of Molecular Devices

BioPrime Plus Array CGH Indirect Genomic Labeling Module from Invitrogen

Description:...ovide a flexible solution to your genomic labeling needs. Using the BioPrime Plus Array CGH Genomic Labeling Systems, you can expect: High yields of fluorescently labeled genomic DNA • signal-to-noise ratios • detection of gene copy number variations. These systems combine a highly concentrated exo-Kl...
Company:Invitrogen

BioPrime Array CGH Genomic Labeling System from Invitrogen

Description:...rovide a flexible solution to your genomic labeling needs. Using the BioPrime Plus Array CGH Genomic Labeling Systems, you can expect: High yields of fluorescently labeled genomic DNA • signal-to-noise ratios • detection of gene copy number variations. These systems combine a highly concentrated exo-K...
Company:Invitrogen

PolarScreen Progesterone Receptor Competitor Assay Kit, Far Red from Molecular Probes (Invitrogen)

Description:...genous assay utilizes a fusion of glutathione transferase to the ligand binding domain of human progesterone receptor [PR-LBD(GST)] and a proprietary, fluorescently tagged progesterone ligand, Fluormone PL Green, PL Red, or PL Far Red. Intrinsic background fluorescence from library compounds is substantially reduc...
Company:Molecular Probes (Invitrogen)

KODAK Image Station 4000MM Digital Imaging System from Molecular Imaging Systems, Carestream Health, Inc. (formerly Kodak Molecular Imaging Systems)

Description:...lengths (green to near-IR) improves the penetration of light into tissue, enabling whole body in-vivo molecular imaging research via the detection of fluorescently tagged biomolecules in living animals and plants. The IS4000R contains broadband UV illumination capability to image UV fluorescence dyes as well as c...
Company:Molecular Imaging Systems, Carestream Health, Inc. (formerly Kodak Molecular Imaging Systems)

Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.

Description:...fluorescence scanners. Sequencing reactions involve a termination step followed by standard slab gel electrophoresis and scanning of the gel to detect fluorescently tagged sequencing products. The kit components have been optimized for sequencing directly from single bacterial colonies or plaques of M13 or lambda...
Company:USB Corp.
Fluorescently in Biological Definition

Mitochondrion

...1 to 4 m in length. At the other end of the spectrum, mitochondria can appear as a highly branched, interconnected tubular network. Observations of fluorescently labelled mitochondria in living cells have shown them to be dynamic organelles capable of dramatic changes in shape. Finally, mitochondria can fuse w...

Mitochondrion

...1 to 4 m in length. At the other end of the spectrum, mitochondria can appear as a highly branched, interconnected tubular network. Observations of fluorescently labelled mitochondria in living cells have shown them to be dynamic organelles capable of dramatic changes in shape. Finally, mitochondria can fuse w...
Fluorescently in Biological Dictionary

Sensory neurons

...pects of the world, such as ... Abstract— Sensory neurons that innervate the heart sense ischemia and mediate angina. ... on these cells, we fluorescently labeled cardiac sensory neurons (CSNs) ... Peripheral sensory neurons detect diverse physical stimuli and transmit the ... All peripheral ...

Oligodeoxyribonucleotide

...s ... An antisense oligonucleotide phosphorothioate, previously shown to inhibit HIV-1 viral expression in chronically infected H9 cells, was fluorescently labeled to ... ... modified raf antisense oligodeoxyribonucleotide encapsulated in a novel cationic ... oligodeoxyribonucleotide is associate...

Nick translation

...on LinkedIn. ... Nick has been working as a documentation professional in the translation and ... When doing a nick translation reaction to fluorescently label DNA probes for FISH analysis, the tubes containing a mixture of dNTPs, ... ... Nick Arce 1 comments Links to this post. Cost In Translatio...

In situ hybridization

... often desirable ... Multicolor fluorescence in situ hybridization (FISH), in its simplest form, can ... In situ hybridization kits and fluorescently labeled probes are commercially ... RPA/RNAi/ In Situ Hybridization Research Core ... IN SITU HYBRIDIZATION is an imaging method to visu...
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