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Flasks in Medical News

Bioreactors might solve blood-platelet supply problems

...everal layers for gas and growth-media control. Control cells were grown in culture flasks. Other attempts to grow platelets have usually used culture flasks or similar two-dimensional systems. After a few days of growth, a solution of growth factors was added to both groups to stimulate the cells to form ...

Zinc finger proteins put personalized HIV therapy within reach

...human CD4 T cells and added DNA that expresses the zinc fingers, which modifies the CCR5 co-receptor. They grew the engineered cells in tissue culture flasks and transferred them into immune-deficient mice infected with HIV. "We followed them over time and showed that those mice that received the zinc-finge...

Pfizer's work on penicillin for World War II becomes a National Historic Chemical Landmark

...fermentation proved critical when Allied governments sent out the call for penicillin. Initially, Pfizer researchers, led by Jasper Kane, used shallow flasks and pans like those that were used for citric acid, and they made gradual progress in improving penicillin's potency and purity. The breakthrough came...

Cell growth technology promises more successful drug development

... the moment, most drugs in development are first tested on cells grown in two-dimensions (2D) in standard laboratory equipment such as Petri dishes or flasks but cells in the human body form tissues and grow in more complex, three-dimensional ways. The new study tested the toxic effect of a cancer drug c...

Northern Forests Less Effective Than Tropical Forests in Reducing Global Warming

...eculate about a "missing carbon sink" in the north. To test whether the computer models were correct, Stephens and his collaborators turned to flasks of air that had been collected by research aircraft over various points of the globe. The air samples had been collected and analyzed by seven...

Predicting the Dynamics of Clotting in Human Blood

...rong spot can block blood vessels and can be life-threatening." In the past, scientists have typically examined the blood-clotting network using flasks containing homogenous mixtures—the test fluids were the same throughout. But the contents of the circulatory system are not homogeneous, said Kastrup,...
Flasks in Medical Products

Concept Plus

Description:... The Concept Plus also allows users to transfer large bottles, flasks and other equipment, quickly and efficiently. The front-loading interlock features class leading one touch operation and easy access for loading/unloa...
Company:Biotrace, Inc.

UVS800DDA Universal Vacuum Source

Description:...g connections. TEFLON materials and ETFE-coated materials ensure chemical resistance and durability Utilizes interchangeable GCF400 Glass flasks to easily remove captured solvents and provide quick changeover to eliminate the need for defrosting Simple-to-use design ...
Company:ThermoFisher Scientific

miniTHERM CTT (120V / 60Hz)

Description:... from 0.1 to 20 rpm. Shelves with and without rollers are interchangeable. Consequently, it is possible to simultaneously incubate static cell culture flasks and rotating miniPERM bioreactors....
Company:Sartorius AG

miniTHERM CTT (230V / 50Hz)

Description:... from 0.1 to 20 rpm. Shelves with and without rollers are interchangeable. Consequently, it is possible to simultaneously incubate static cell culture flasks and rotating miniPERM bioreactors....
Company:Sartorius AG

SR48336 Reciprocating Shaker

Features:...e driven by a unique counterbalanced drive system allowing constant long-term operation without maintenance. Racks & clips are available to suit most flasks and containers. Optional alarm systems can be individually selected. ...
Company:Axyos

SR4136 Reciprocating Shaker

Features:...e driven by a unique counterbalanced drive system allowing constant long-term operation without maintenance. Racks & clips are available to suit most flasks and containers. Optional alarm systems can be individually selected. ...
Company:Axyos

UV Sterilizing PCR Workstations

Features:...ective for protection against 32P labeled compounds The workstation is designed for placement of large instruments on the base work area or tubes, flasks and pipettes on the two removable shelves The four built-in power outlets allow operation of equipment for use in PCR applications ...
Company:UVP, Inc.
Flasks in Biological News

Methane-eating microbes can use iron and manganese oxides to 'breathe'

...ree but contained iron oxide or manganese oxide. She placed methane gas that contained the non-radioactive carbon-13 isotope in the empty space in the flasks above the sediment and tested any resulting carbon dioxide produced by the samples. All the carbon dioxide had the carbon-13 isotope and so came from ...

Volcanoes may have provided sparks and chemistry for first life

...ton, Ind. From 1953 to 1954, Professor Stanley Miller, then at the University of Chicago, performed a series of experiments with a system of closed flasks containing water and a gas of simple molecules. At the time, the molecules used in the experiment (hydrogen, methane, and ammonia) were thought to be ...

Researchers create system to build transplant tissue

...embedding this mimic of a microvascular system allows you to maintain the core of the tissue during culture." Gel scaffolds, he said, "are the culture flasks of the future." The embedded microchannels allow fluid with oxygen, sugar and proteins to travel through the system. The researchers can control the ...

Scientists close in on missing carbon sink

...rbon sinks, focusing in particular on estimates produced by a recent international study into global carbon exchange known as TransCom. They turned to flasks of air collected by research aircraft over various points of the globe for the past 27 years. The air samples had been analyzed by several labs around...

War on terror meets war on cancer

...at the water inside a cell is indistinguishable from water outside a cell," Hegg says. The researchers grew E. coli bacteria at body temperature in flasks containing a nutrient-rich liquid culture medium. There were four sets of flasks, each holding a growth medium containing water with a different ratio...

Engineers improve plastic's potential for use in implants by linking it to biological material

...iece, or peptide, was linked to a smaller protein piece that human cells like to attach to, polypyrrole gained the ability to attach to cells grown in flasks in the laboratory. "It will be very useful from a biomedical standpoint to be able to link factors to polypyrrole in the future that stimulate nerve...
Flasks in Biological Technology

Fundamentals of Fermentation: Techniques For Benchtop Fermentors

... Question: What kind of MEDIA should be used, and does it differ from media used in shake flasks? Answer: The media used in shake flasks does differ from the standard media used in a fermentation vessel. Shake flask media is generally of a much simpler composition. L...

HEK293 Calcium Flux Assay

...ptomycin and 2 mM L-glutamine. Cell monolayers were grown in T150 flasks and were maintained at 5% CO 2 and 37 oC and passaged at 50-70% confluency... cells in prewarmed growth medium to a total volume of 12 mL. Place two T75 flasks per unattended run in an incubator at 10% CO 2 in air and 37 o C. The fla...

Calcium Flux Assays using the LabChip 3000

... were maintained at 37 oC and 5% CO 2 and subcultured in monolayers in T75 flasks using standard cell culture methodologies. Cells were split at 80-90% confl...ency. The day before each assay cells were seeded at 3 x 106 cells into T75 flasks and incubated overnight at 37 oC and 5% CO 2 . 2. Label, Suspend...

Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

... CHO cells (passage number > 40) were routinely grown in 75 cm 2 flasks as a monolayer in Hams-F12 medium supplemented with 10% foetal bovine serum...d solution (Molecular Probes, cat # P3000) Plasticware : 75cm 2 culture flasks (Corning cat # 430641), 96-well black walled, clear bottomed plates (Costar...

Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

...ion of GFP) using Lipofectamine as per manufacturer's instructions (Gibco/Invitrogen, Cat. No. 18324-012). Cells were grown to confluence in 162 cm 2 flasks under standard cell-culture conditions. Fluorescence microscopy revealed an expression level in excess of 75 %. The GFP-expressing cells ...

measurement of green fluorescent proteins and a red fluorescent protein in live cells in the Analyst GT multimode reader

...Multimode Reader (Molecular Devices) METHODS Cell preparation and analysis The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 g/mL of G418. Non-transfected HEK cells were included as controls. The night before the experiment, ...

Counting Cytostar-T scintillating microplates on the MicroBeta JET

...nate and domoate. BHK cells (supplied by Novo Nordisk) transfected with the inducible GluR6 ion channel were grown as monolayers in 75 cm 2 flasks at 37 C in a humidified 5% CO 2 incubator. GluR6 expression was induced in the cells with 5 mM (final concentration) isopropyl- 1-thio-β-D-galac...

Electrotransformation of E. coli With M13 DNA

... 10 g/ml tetracycline. Bacteria were grown at 37 C with moderate agitation until they reached an O.D. 600 of approximately 0.3. flasks were chilled on ice for 30 minutes, and cells were spun at 3,000 rpm in 250 ml bottles. The supernatants were decanted, and cell...

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

... Materials and Methods E. coli strain JS4 6 was grown at 37 C in LB broth; in mid-log phase (OD 600 =0.6) flasks were placed in ice water and chilled for 15 minutes. Cells were harvested, washed in ice-cold 1 mM HEPES, pH 7.0 (first wash 1x, second ...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

... Materials and Methods The Sf9 insect cells containing the TGEV proteins were harvested from 150 cm 2 stationary cell culture flasks (1.8 x 10 7 cells) and resuspended in 1 ml of phosphate buffer saline (PBS), pH 7.3, mixed with 4 parts of sample buffer (0.06 ...
Flasks in Biological Products

BD BioCoat Vented Caps for 175 cm2 Flasks from BD Biosciences - Discovery Labware

Description: BD BioCoat Vented Caps for 175 cm2 Flasks...
Company:BD Biosciences - Discovery Labware

Vented Caps for 75 cm2 Flasks from BD Biosciences - Discovery Labware

Description: Vented Caps for 75 cm2 Flasks...
Company:BD Biosciences - Discovery Labware

CERTOMAT R from Sartorius AG

Description:...ive Shaking speed: 40 - 350 rpm 25 mm amplitude Clamps for Erlenmeyer flasks 25 ml to 2000 ml, including Fernbach flasks Hinged racks for test tubes and centrifugation tubes ...
Company:Sartorius AG

CERTOMAT U from Sartorius AG

Description:...ive Shaking speed: 40 - 350 rpm 25 mm amplitude Clamps for Erlenmeyer flasks 25 ml to 2000 ml, including Fernbach flasks Hinged racks for test tubes and centrifugation tubes ...
Company:Sartorius AG

Pyrex brand shaker flask with extra-deep baffles from Sigma-Aldrich

Description:... These 50ml PYREX Delong style shaker flasks have three extra deep baffles designed to provide greater agitation of solu...en mechanical strength and thermal shock resistance. For convenience, these flasks include a marking spot. The approximate neck O.D. is 18mm. • Approx. O...
Company:Sigma-Aldrich

TriEx Sf9 Cells from Novagen

Description:...nsect Cells and BacVector Insect Cell Medium. After recovery from the frozen stock, the cells can be grown as semi-adherent cultures in tissue culture flasks or in suspension as shaker cultures. Each vial contains 2 x 10 6 cells. Cells are shipped on dry ice. Upon receipt, the vials should be removed from ...
Company:Novagen

Pyrex brand baffled trypsinizing flask, with beaded neck from Sigma-Aldrich

Description:... These 1.5L PYREX trypsinizing flasks are used for converting homogenous tissue samples into cell suspensions by digestion of connective proteins. Heavy beaded neck accepts cotton plugs. F...
Company:Sigma-Aldrich

Pyrex brand Fernback baffled culture flask, with screw cap from Sigma-Aldrich

Description:... These 2800mL PYREX Fernbach-style culture flasks have triple baffles located at the center of the flask bottom to achieve maximal oxygen transfer to culture medium. These flasks have a large marking spot. A wide range of optional caps are available for...
Company:Sigma-Aldrich

Pyrex brand Fernback baffled culture flask from Sigma-Aldrich

Description:...ese 2800mL wide mouth PYREX Fernbach-style culture flasks have triple baffles located at the center of the f...n transfer to culture medium. These triple baffled flasks have a Delong-style 38mm neck for use with plastic... closures see Nos. CLS2679638 or CLS2679738. These flasks have a large marking spot....
Company:Sigma-Aldrich

VSN-5 Variable Speed Nutating Mixer, 115V .25 amp (without table) from PRO Scientific

Description:...movable nipple rubber mat for various tube sizes or an 8" x 8" table with removable flat rubber mat for Microwell plates, blots, petri dishes and cell flasks is available for the VSN-5. The PRO VSN-5 is constructed of ABS plastic, comes equipped with 4 non-slip rubber suction cup feet to safely keep your ...
Company:PRO Scientific
Flasks in Biological Definition

Aseptic technique in microbiology

...the specimen itself, until the entire procedure is finished. Preparing to execute the specimen transfer, the person would hold both of the tubes or flasks in one hand , probably the opposite of the writing hand. The person would then open the tube or flask containing the specimen source and briefly ho...
Flasks in Biological Dictionary

Ampulla

...a "small nearly globular flask or bottle, with two handles" (OED). The word is used of these in archaeology, and of later, often handle-less flasks for holy water or holy oil in the Middle Ages, often bought as souvenirs of pilgrimages. The ampulla of Vater, also known as the hepatopancreatic...
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