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FlashPlates in Biological Technology

Counting FlashPlates on the Wallac MicroBeta

FlashPlates and ScintiPlates are popular platforms for running a variety of radiometric screening assays. The Wallac MicroBeta effectively measures both plate types. MicroBeta detectors comprise two photomultiplier tubes, one above the sample and one below. ...

In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate

...obot to all wells. After overnight incubation, the flashplates were placed onto a Packard TopCount Microplate S...riation (Table 1). Inter-assay variation using the flashplates was minimal, with only 6% error on three separate ...ors full automation. The results obtained from the flashplates are very reproducible and comparable to those obta...

Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor

...es of recombinant hTRIIs-Fc for TGF-1 using FlashPlate technology. For our experiments, the hTRIIs-Fc component was coated onto the wells of Protein A flashplates and incubated with 125 I-labeled TGF-1. The key feature of this Protein A FlashPlate binding assay, as described here, is its simplicity comprising ...

Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

... Intra-assay variation was investigated by measuring the stimulation of [ 35 S]-GTPγS binding produced by 5-HT [10 μM] in 80 of the flashplates 96 wells. Figure 2a shows the data in terms of absolute counts per minute; Figure 2b shows the same data with each row expressed as a percentage of it...
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